FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1302887
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT82850.6358955151137435No Hit
GTATAATATTGCTGACAGTGATAAACTGCCACATCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAATGTGTC55560.4264375958928134No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51630.39627381346195023No Hit
ATACTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA45300.3476894005389569No Hit
GGGTAGTATAATATTGCTGACAGTGATAAACTGCCACATCTTCAGGCTGC38850.2981839560913571No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36080.2769234783983569No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33930.26042166358249025No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33540.2574283111275191No Hit
GTAATAGACGGCTGTGTCTTCGATTTCCAGGCTGTTCATTTGCAGATATA32340.24821799588145405No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27490.21099297176194096No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27270.20930441396682906No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGTTTCGCTTTCGGTAACGCCTGG26530.20362471956508893No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26120.20047786185601665No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24640.1891184730525364No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGTTGGTACCAAGCTAAGTAATTTTTATTGTTGAAACTGTATAAAATACT24600.18881146254433423No Hit
ATTTTATACAGTTTCAACAATAAAAATTACTTAGCTTGGTACCAACAGAA24390.18719965737627284No Hit
CATTTCACTCTCACCATCAACAGCCTGCAGCCTGAAGATGTGGCAGTTTATCACTGTCAGCAATATTATACTACC24390.18719965737627284No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGACCCGGGATCCCT24300.18650888373281796No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24100.1849738311918071No Hit
GTCTATTACTGTGCCGCAGTCAAGGCATACTATGATAACGATGGTTATTTCTCAAACTGGTTCGACCCCTGGGGC23930.1836690365319479No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23150.17768233162200558No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22420.172079389847316No Hit
GTTTATCACTGTCAGCAATATTATACTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA22350.17154212145796222No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22050.16923954264644595No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20980.16102701155203789No Hit
CTCCTGTGCAGCCTCTGGTTTCGCTTTCGGTAACGCCTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGC20170.15481004876094398No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19830.15220045944122554No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19600.15043514901906305No Hit
GTGATAAACTGCCACATCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAATGTGTCCCAGACCCGCTGCCA19490.14959087012150707No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19200.14736504393704136No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18600.1427598863140088No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18570.14252962843285719No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18130.1391525128426333No Hit
GGCCTGGAGTGGGTCGGCCGTATTAAGGGCAAAACTGATGGTGAGACAAT17710.13592890250651055No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17280.13262853954333723No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16840.12925142395311334No Hit
GGACTGAACACAGAGGACTCCCCATGGAGTTTGGGCTGAGCTGGATTTTC16680.12802338192030469No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16160.12403224531367647No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC15720.1206551297234526No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15580.119580592944745No Hit
GAGTGAAATGTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGATCCCG15530.1191968298094923No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15520.11912007718244176No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG15450.11858280879308797No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15160.11635698260862223No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14970.11489868269466193No Hit
GTTGATGGTGAGAGTGAAATGTGTCCCAGACCCGCTGCCACTGAATCGGT14810.11367064066185326No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14780.11344038278070162No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14780.11344038278070162No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTATTTTATA14740.11313337227249946No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14740.11313337227249946No Hit
GCCTGAAGATGTGGCAGTTTATCACTGTCAGCAATATTATACTACCCCGC14740.11313337227249946No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14640.11236584600199404No Hit
CTCCAGGCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAGGCGTTAC14620.11221234074789295No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14570.11182857761264024No Hit
CACCATCAACAGCCTGCAGCCTGAAGATGTGGCAGTTTATCACTGTCAGCAATATTATACTACCCCGCTCACTTT14310.10983300930932614No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14300.1097562566822756No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGA14140.10852821464946692No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTATTTTATACAGTTTCAA13930.10691640948140553No Hit
TCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAATGTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGATC13890.10660939897320335No Hit
ATTCAGTGGCAGCGGGTCTGGGACACATTTCACTCTCACCATCAACAGCC13810.10599537795679902No Hit
GGGTTCCCTGGCCCCAGGGGTCGAACCAGTTTGAGAAATAACCATCGTTA13720.10530460431334415No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13420.10300202550182785No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13390.10277176762067623No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13390.10277176762067623No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG13230.10154372558786756No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13080.10039243618210941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11500.041.524531
TCTTGAG11100.041.35326470
CGACGAA350.001246516440.0605254
GCCCGAT654.979447E-737.5861951
CGGCAAA951.2006086E-437.1677170
GTGGTAT3350.036.6549151
GTATCAA25200.034.9215661
TGCCCGA751.5449878E-632.5210350
TGGTATC3700.032.2644422
GACAGGT2550.030.7653711
CTCTTGA11650.029.82791369
TGCACAG8800.029.69193370
ATCAACG30050.029.6235543
CCCAACG500.005485892329.61163167
TATCAAC30150.029.5264382
TTTCGAT1204.7316373E-429.42443770
AACGCAG30750.029.0612346
CAACGCA31150.029.0154295
TCAACGC30800.029.0129414
ACGCAGA32400.027.5812637
Produced by FastQC (version 0.11.5)