FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1540975
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTGTACATTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAACAATCGGCC83530.5420594104381966No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA46160.2995506091922322No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT44620.28955693635522967No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG38160.2476354256233878No Hit
CTCTTGTACTGGGAGCGCCTCCAACATCGGGACACAGTATAGTGTACATTGGTACCAGCAACTTCCAGGAACAGC37340.24231411930758123No Hit
GTCCTGGGCCCAGTCTGTGCTGGCGCAGCCGCCCTCAGTGTCTGGCGCCC36740.23842048053991793No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC34950.22680445821638898No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC33130.2149937539544769No Hit
ACACTATACTGTGTCCCGATGTTGGAGGCGCTCCCAGTACAAGAGATGGT31960.20740115835753337No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC31490.20435114132286375No Hit
CAGTATAGTGTACATTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAACAATCGG29400.19078829961550317No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC28310.18371485585424813No Hit
ACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAAACATAACTACTCAGGC25800.16742646700952318No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT24250.15736790019305957No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC23540.15276042765132464No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22780.14782848521228442No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22630.14685507552036858No Hit
GATTATTACTGCCAGTCGTATGACATCAGCCTGAGTAGTTATGTTTTCGGAACTGGGACCAAGGTCACCGTCCTA22540.1462710297052191No Hit
ACATCAGCCTGAGTAGTTATGTTTTCGGAACTGGGACCAAGGTCACCGTCCTACGTCAGCCCAAGGCCAACCCCA21990.14270186083486103No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21650.14049546553318518No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG21610.14023588961534092No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT21590.14010610165641882No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21380.13874332808773668No Hit
GTGCTGGCGCAGCCGCCCTCAGTGTCTGGCGCCCCAGGGCAGTGGGTCAC21160.13731566053959346No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCTTGGTC21130.13712097860121028No Hit
GTCTCTGGTGGCTCCATCAGCGCTGGTGGTTATGCCTGGACCTGGATCCGGCAGTCACCGGGGAAGGGCCTGGA20490.13296776391570272No Hit
GTACAAGAGATGGTGACCCACTGCCCTGGGGCGCCAGACACTGAGGGCGG20250.1314103084086374No Hit
GTGTACATTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCATC20150.13076136861402682No Hit
GACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAAACATAACTACTCAGG19730.12803582147666248No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG19180.12446665260630445No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGGCGCAGCCGCCCTCAGTGTCTGGCGCCCCAGGGCAGTGGGTCAC18860.12239004526355067No Hit
ACACAGTATAGTGTACATTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAACAAT18760.12174110546894013No Hit
GGCTGATGTCATACGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGG18340.11901555833157579No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT17620.11434319181037979No Hit
GCCTTGGGCTGACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAAACATAACTACTCAGGCTGATGTCATACGA17440.11317510018008078No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG17440.11317510018008078No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGGCGCAGCCGCCCTCAGTGTCT17330.11246126640600917No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC17320.11239637242654812No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17130.11116338681678808No Hit
GGATGAGGCTGATTATTACTGCCAGTCGTATGACATCAGCCTGAGTAGTTATGTTTTCGGAACTGGGACCAAGGT17110.11103359885786596No Hit
GTGATGGCCAGGGAAGCTGAGGTGCCAGACTTGGAGCCACTGAATCGGTC17000.11031976508379435No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16940.10993040120702803No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16770.10882720355619008No Hit
CCCCAGGGCAGTGGGTCACCATCTCTTGTACTGGGAGCGCCTCCAACATC16700.10837294569996268No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC16560.1074644299875079No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC16540.1073346420285858No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA16240.10538782264475413No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAAGCTGAGGTGCCAGACTTGGAGCCA15880.10305163938415614No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT15750.10220801765116241No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAA752.5681973E-548.274870
GGTATCA10700.043.983181
GTGGTAT2850.040.391471
CGATAAC350.001236201340.1287350
TCCGATA350.001261788239.96189548
CGCGTAT451.02647085E-438.17474439
GTATCAA25350.035.9277461
ATAACGA400.002324932535.2860952
TGGTATC3300.034.8643042
CTTATAC5250.034.8249171
AATTCCG400.002503819334.7568945
GCCGTCT3750.034.3564446
ATCGCGT501.9470844E-434.23770537
CGTATGC4550.034.1247441
TCTCGTA4000.033.44544638
CTCTTAT2650.033.2187351
TCTTATA2050.033.0135422
TACACAT6050.032.99995
GATCTCG3800.032.38362536
ATGCCGT4400.032.33429744
Produced by FastQC (version 0.11.5)