FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1540975
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTGTACATTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAACAATCGGCC80210.5205146092571261No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55940.3630169211051445No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA43670.2833920083064294No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT41850.2715813040445173No Hit
GTCCTGGGCCCAGTCTGTGCTGGCGCAGCCGCCCTCAGTGTCTGGCGCCC38360.2489333052126089No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC38250.2482194714385373No Hit
CTCTTGTACTGGGAGCGCCTCCAACATCGGGACACAGTATAGTGTACATTGGTACCAGCAACTTCCAGGAACAGC37990.24653222797254987No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG37250.24173007349243175No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC35430.22991936923051964No Hit
ACACTATACTGTGTCCCGATGTTGGAGGCGCTCCCAGTACAAGAGATGGT33430.21694057333830852No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC31330.20331283765148686No Hit
CAGTATAGTGTACATTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAACAATCGG28150.18267655218287124No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC26780.17378607699670665No Hit
ACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAAACATAACTACTCAGGC25090.16281899446778825No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG22310.14477846817761483No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22180.14393484644462112No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21660.1405603595126462No Hit
GTGCTGGCGCAGCCGCCCTCAGTGTCTGGCGCCCCAGGGCAGTGGGTCAC21600.1401709956358799No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCTTGGTC21590.14010610165641882No Hit
GATTATTACTGCCAGTCGTATGACATCAGCCTGAGTAGTTATGTTTTCGGAACTGGGACCAAGGTCACCGTCCTA21500.1395220558412693No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC21350.1385486461493535No Hit
GTACAAGAGATGGTGACCCACTGCCCTGGGGCGCCAGACACTGAGGGCGG21160.13731566053959346No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21100.1369262966628271No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT21060.13666672074498287No Hit
GGCTGATGTCATACGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGG20880.13549862911468388No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT20760.13471990136115122No Hit
GTCTCTGGTGGCTCCATCAGCGCTGGTGGTTATGCCTGGACCTGGATCCGGCAGTCACCGGGGAAGGGCCTGGA20460.13277308197731955No Hit
GTGTACATTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCATC20140.1306964746345658No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19280.12511559240091502No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGGCGCAGCCGCCCTCAGTGTCTGGCGCCCCAGGGCAGTGGGTCAC18980.12316877301708334No Hit
GCCTTGGGCTGACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAAACATAACTACTCAGGCTGATGTCATACGA18860.12239004526355067No Hit
ACATCAGCCTGAGTAGTTATGTTTTCGGAACTGGGACCAAGGTCACCGTCCTACGTCAGCCCAAGGCCAACCCCA18840.12226025730462857No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18740.121611317510018No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18240.11836661853696523No Hit
GACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAAACATAACTACTCAGG18220.11823683057804311No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT18020.11693895098882201No Hit
GTGATGGCCAGGGAAGCTGAGGTGCCAGACTTGGAGCCACTGAATCGGTC17750.11518681354337351No Hit
GTCTGTGCTGGCGCAGCCGCCCTCAGTGTCTGGCGCCCCAGGGCAGTGGGTCACCATCTCTTGTACTGGGAGCGC17450.11323999415954185No Hit
ACACAGTATAGTGTACATTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAACAAT17410.11298041824169763No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17290.11220169048816496No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17110.11103359885786596No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16800.10902188549457324No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGGCGCAGCCGCCCTCAGTGTCT16730.10856762763834585No Hit
GGATGAGGCTGATTATTACTGCCAGTCGTATGACATCAGCCTGAGTAGTTATGTTTTCGGAACTGGGACCAAGGT16450.10675059621343629No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAAGCTGAGGTGCCAGACTTGGAGCCA16080.10434951897337726No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC15770.10233780561008453No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15440.10019630428786969No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)