FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920725
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGGTTACCCGTGGACGTTCGGCCAAGGGACCACGGTGGAAATCAAACGAACTGTGGCTGCACCAT64570.7012951749979636No Hit
CCATTATACTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC53740.5836704770697005No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34920.3792663390263108No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26920.2923782888484618No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23820.2587091694045453No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22320.24241765999619863No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22190.24100572918080862No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22140.24046267886719705No Hit
GATCTATGCAGCATCCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA20150.2188492763854571No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18550.2014716663498873No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17780.19310869152026935No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC17380.1887642890113769No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15970.17345027016753103No Hit
GTAGGAGACAGAGTCACCATGACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAACAGGAA15960.17334166010480873No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATGACTT15270.16584756577696924No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15150.16454424502430148No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14960.1624806538325776No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14410.15650710038285048No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14230.15455211925384887No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14110.15324879850118114No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13980.15183686768579108No Hit
GCATAGATCAGGCGCTTAGGGGCTTTCCCTGGTTCCTGTTGATACCAGCC13970.15172825762306877No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13270.14412555323250698No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13160.14293084254256158No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13020.1414103016644492No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAGTATAATGGTTAC12730.1382606098455022No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12710.13804338972005756No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACAT12580.13663145890466752No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12420.13489369790111053No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11790.12805126394960495No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11580.12577045263243639No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11250.12218632056260012No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCGTGGTCC11070.12023133943359852No Hit
ACTTATTACTGTCTACAGTATAATGGTTACCCGTGGACGTTCGGCCAAGGGACCACGGTGGAAATCAAACGAA11060.12012272937087622No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCGTGGTCCCTTGGCCGAACGTC11000.11947106899454234No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10570.11480083629748297No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA10540.11447500610931602No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG10420.1131716853566483No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10140.1101306036004236No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9950.10806701240869968No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9750.10589481115425345No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9750.10589481115425345No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9740.10578620109153113No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9660.10491732058975264No Hit
GGTATCAACAGGAACCAGGGAAAGCCCCTAAGCGCCTGATCTATGCAGCATCCAGTTTACAAAGTGGGGTCCCAT9550.10372260989980721No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACATCCAGATGACCCAGTCTCCATCCTCC9410.10220206902169487No Hit
GTAATACATGGCGGTGTCCGAGGCCTGCAGGCTGCTCCACTGCAGGTAGG9400.10209345895897254No Hit
ATACTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGA9270.1006815281435825No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATGACTTGCCGGGCAAGTCAGG9230.10024708789269327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2750.042.0969921
ATTCTAA652.539416E-531.429881
ATCAACG24850.029.3219073
TCAACGC24850.029.1817194
CAACGCA24950.028.9283035
AACGCAG25450.028.3599666
TCTGAGT2553.3887773E-928.11999170
ATTATAC16950.027.5204623
TATACTG16950.027.517475
GGATATA1050.00812903827.31656370
TTATACT17150.027.1965664
TACCCGT21300.027.16790811
TAATGGT21500.027.077924
TATAATG21700.026.9867122
ACGCAGA26950.026.907827
GGTTACC21700.026.8283548
CGCAGAG27150.026.7096028
ATAATGG21900.026.5862353
GTATAAT22300.026.5674111
CGTTCGG21800.026.41878121
Produced by FastQC (version 0.11.5)