FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345503
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66410.49357006264571684No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64550.4797462361659543No Hit
CTATGAAGCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTAAATT47690.35443993807520313No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45230.336156812731001No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42500.31586700289780106No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42340.31467785653395053No Hit
GCTTCATAGTTATCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAAAGAACTGTGGCTGCACCAT38190.28383437272157697No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTT38180.2837600510738363No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG36410.27060511942373966No Hit
GAATTTACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTACTGTCAACAGCTTCATAGTTAT35030.2603487320355287No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT34710.25797043930782765No Hit
GTGCAATCACTTTGCAAGGCGGGGTCCCATCGAGGTTCAGCGGCAGTGGA33290.24741676532865406No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG33130.2462276189648035No Hit
GTGATTGCACCATGGATCAGGAGTTTAGGGACTTTTCCTGGTTTTTGTTGATACCAGGCTATGTAATTGCTAATG32110.23864681089525627No Hit
GTAGGAGACAGAGTCACCATCAGTTGCCGGGCCAGTCAGGGCATTAGCAATTACATAGCCTGGTATCAACAAAAA31930.2373090212359244No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31360.23307268731470684No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30450.22630941737030685No Hit
ACTTACTACTGTCAACAGCTTCATAGTTATCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAAAGAA28860.21449227537954207No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG28820.21419498878857945No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28260.21003297651510253No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27590.2050534261164784No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT27170.2019319169113707No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26520.19710100980822784No Hit
GTAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTAAATTCTGTCTCAGATCCACTGCCG26500.19695236651274653No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25990.1931619624779729No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT25450.18914859349997734No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25100.18654733582905428No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25040.18610140594261032No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24560.1825339668510587No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCGTTC23640.17569637525891804No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23580.17525044537247408No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23250.17279783099703233No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22220.16514270127974445No Hit
GAGTAAATTCTGTCTCAGATCCACTGCCGCTGAACCTCGATGGGACCCCG22150.16462244974555984No Hit
ATGTAATTGCTAATGCCCTGACTGGCCCGGCAACTGATGGTGACTCTGTC21840.1623184786655994No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20690.1537714891754236No Hit
CTCCTGATCCATGGTGCAATCACTTTGCAAGGCGGGGTCCCATCGAGGTT20630.15332555928897965No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20460.15206209127738846No Hit
GATCAGGAGTTTAGGGACTTTTCCTGGTTTTTGTTGATACCAGGCTATGT20370.15139319644772253No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT20250.15050133667483462No Hit
GGGCATTAGCAATTACATAGCCTGGTATCAACAAAAACCAGGAAAAGTCCCTAAACTCCTGATCCATGGTGCAAT20050.14901490372002144No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19720.14656228934457968No Hit
GCCTGGTATCAACAAAAACCAGGAAAAGTCCCTAAACTCCTGATCCATGGTGCAATCACTTTGCAAGGCGGGGTC19070.14173138224143686No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18720.13913012457051377No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18690.1389071596272918No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18600.13823826479762588No Hit
GTTTAGGGACTTTTCCTGGTTTTTGTTGATACCAGGCTATGTAATTGCTA18510.13756936996795993No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18440.13704911843377535No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC18060.1342248958196303No Hit
CAATTACATAGCCTGGTATCAACAAAAACCAGGAAAAGTCCCTAAACTCC17900.13303574945577973No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17550.13043449178485667No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16950.12597519292041712No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16810.12493468985204789No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG16770.12463740326108526No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16740.1244144383178633No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16620.12352257854497538No Hit
GTGCAGCCACAGTTCTTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG16560.12307664865853142No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16430.12211046723790285No Hit
GTCTCCATCGTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCAGTT16300.12114428581727428No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16250.12077267757857099No Hit
GTCTCCTACAGATGCAGACAGGAACGATGGAGACTGGGTCAACTGGATGTCACATCTGGCACCTGGGAGCCAGAG16180.12025242604438637No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCTTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGATAA16000.1189146363850545No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15890.11809709825990726No Hit
CCATCGTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCAGTTGCCG15780.11727956013476001No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15740.11698227354379738No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15600.11594177047542814No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15590.11586744882768749No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15580.11579312717994682No Hit
GTCCCTAAACTCCTGATCCATGGTGCAATCACTTTGCAAGGCGGGGTCCCATCGAGGTTCAGCGGCAGTGGAT15230.11319186950902375No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15190.11289458291806112No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15120.11237433138387651No Hit
GAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT14920.11088789842906334No Hit
ATACCAGGCTATGTAATTGCTAATGCCCTGACTGGCCCGGCAACTGATGGTGACTCTGTCTCCTACAGATGCAGA14360.10672588615558641No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGCGGATAACTATGAAGCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGG14340.10657724286010511No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14340.10657724286010511No Hit
GTACTAGCCTCTGGAACGGTCTTTGACTTCTGGGGCCAGGGAACGCTGGT14260.10598266967817983No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14230.10575970473495787No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14030.10427327178014467No Hit
GACCAAGGTGGAGATCAAAAGAACTGTGGCTGCACCATCTGTCTTCATCT13840.10286116047307214No Hit
GTCCCATCGAGGTTCAGCGGCAGTGGATCTGAGACAGAATTTACTCTCAC13810.10263819552985018No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC13740.10211794399566557No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13700.10182065740470293No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13650.10144904916599964No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13600.10107744092729633No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGC200.0079258750.788184
GCCGGTG951.7689472E-646.33652570
TACGTAT656.917659E-445.14840770
GTGGTAT2850.040.3959431
GTACGTA500.00197049136.49456869
GACAGGT2850.033.2672461
GGTACGT500.003856974731.82276568
ATCAACG37000.030.8366573
AACGCAG37600.030.4346246
TCAACGC37600.030.3468384
CAACGCA37950.030.3323615
TGGTACG500.00505054930.11672867
GGTCCAA1000.00573034129.34646470
CGCGGAT500.005849681329.22522465
ACGCAGA39850.028.9711117
CGCAGAG40700.028.3660648
AGAGTAC41600.027.75134511
CAGAGTA42250.027.2442710
GACCGAG500.00847984527.0850167
TTCCGCC500.00848138227.08401111
Produced by FastQC (version 0.11.5)