FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1403659
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT88460.6302100438924269No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54020.38485130647828286No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49620.3535046617447685No Hit
GTATAATATTGCTGACAGTGATAAACTGCCACATCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAATGTGTC49290.3511536633897549No Hit
ATACTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA45730.32579137810536607No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38030.270934749821716No Hit
GGGTAGTATAATATTGCTGACAGTGATAAACTGCCACATCTTCAGGCTGC37640.2681562972203363No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36340.25889478854907066No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGTTGGTACCAAGCTAAGTAATTTTTATTGTTGAAACTGTATAAAATACT34990.2492770680058333No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34460.24550122216293271No Hit
ATTTTATACAGTTTCAACAATAAAAATTACTTAGCTTGGTACCAACAGAA33770.2405854983297225No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGACCCGGGATCCCT33200.23652468298924453No Hit
GTAATAGACGGCTGTGTCTTCGATTTCCAGGCTGTTCATTTGCAGATATA32310.23018411166814734No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30510.2173604842771642No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29770.21208854857198223No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29320.20888264172423646No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29030.20681661286680025No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28870.20567673487649063No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28380.20218585853116747No Hit
CATTTCACTCTCACCATCAACAGCCTGCAGCCTGAAGATGTGGCAGTTTATCACTGTCAGCAATATTATACTACC28140.20047604154570306No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27420.1953465905893098No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26910.1917132294951979No Hit
GTTTATCACTGTCAGCAATATTATACTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA25070.17860463260663736No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24080.17155163754159664No Hit
GTATAAAATACTCTGACTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA23500.16741957982672429No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23460.1671346103291469No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGTTTCGCTTTCGGTAACGCCTGG23390.16663591370838643No Hit
GTCTATTACTGTGCCGCAGTCAAGGCATACTATGATAACGATGGTTATTTCTCAAACTGGTTCGACCCCTGGGGC22270.15865676777621915No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21190.1509625913416293No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21150.15067762184405187No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGA20590.1466880488779682No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20450.1456906556364473No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTATTTTATACAGTTTCAA20290.1445507776461377No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20170.1436958691534055No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19980.14234226403991282No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19820.14120238604960322No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19240.13707032833473087No Hit
GCCTGAAGATGTGGCAGTTTATCACTGTCAGCAATATTATACTACCCCGC19120.13621541984199867No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTATTTTATA18880.13450560285653423No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18270.13015981801847884No Hit
ATTCAGTGGCAGCGGGTCTGGGACACATTTCACTCTCACCATCAACAGCC18040.12852124340740878No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC18020.12837875865862008No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17700.12609900267800087No Hit
CTCCTGTGCAGCCTCTGGTTTCGCTTTCGGTAACGCCTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGC17640.12567154843163475No Hit
GTACCAACAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGACCCGGGATCCCTGA17640.12567154843163475No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17550.1250303670620856No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17170.12232315683510027No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG17130.12203818733752286No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17030.12132576359357936No Hit
CACCATCAACAGCCTGCAGCCTGAAGATGTGGCAGTTTATCACTGTCAGCAATATTATACTACCCCGCTCACTTT16900.12039961272645279No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16820.11982967373129799No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16740.11925973473614318No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16680.11883228048977708No Hit
GGACTGAACACAGAGGACTCCCCATGGAGTTTGGGCTGAGCTGGATTTTC16240.11569761601642566No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16120.11484270752369344No Hit
CTCCAGGCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAGGCGTTAC16000.11398779903096122No Hit
GTGATAAACTGCCACATCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAATGTGTCCCAGACCCGCTGCCA15840.11284792104065161No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15760.1122779820454968No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTATTTTATACAGTTTCAACAATAAAAATTA15580.1109956193063985No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15420.10985574131608887No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15410.10978449894169452No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15390.10964201419290583No Hit
GGATCTCTGGTGCCTACGGGAACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG15330.10921455994653971No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15280.10885834807456797No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15240.10857337857699056No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15170.10807468195623011No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT15130.10778971245865271No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15010.10693480396592049No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14950.1065073497195544No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14850.10579492597561088No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14770.10522498698045607No Hit
GGCCTGGAGTGGGTCGGCCGTATTAAGGGCAAAACTGATGGTGAGACAAT14580.10387138186696342No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGT14530.10351516999499166No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14360.1023040496302877No Hit
GCTTGGTACCAACAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGACCCGGGATC14250.10152038351194984No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14060.10016677839845718No Hit
GTCCTGGTTTCTGTTGGTACCAAGCTAAGTAATTTTTATTGTTGAAACTG14050.10009553602406283No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG200.00726334951.9221444
TACGTAT554.1217293E-450.1491370
GTGGTAT2000.044.3129121
GGTATCA8900.043.6618161
TCGTATG2250.042.88681840
TGGTATC2200.041.8204572
ATGCCGT2500.041.53771244
AATCGCG503.978832E-641.1919136
GACAGGT2750.040.9042241
GCTTGTA857.6397555E-1140.102181
CTTATAC3600.039.7679941
CGTATGC2550.039.2337841
ATCGCGT451.0268361E-438.1721637
TCTTGAG12850.038.0996870
CGAGACG3900.037.58963823
ACGAGAC3900.037.58428222
CGCTCAT3750.037.32681328
TCTCCGA3850.037.1737411
ATCTCCG4000.036.63162210
AATCTCG2650.036.26960836
Produced by FastQC (version 0.11.5)