FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1403659
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT88700.6319198608778912No Hit
GTATAATATTGCTGACAGTGATAAACTGCCACATCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAATGTGTC61020.4347209685543284No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54540.38855590994678907No Hit
ATACTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA44580.3175985050500157No Hit
GGGTAGTATAATATTGCTGACAGTGATAAACTGCCACATCTTCAGGCTGC43480.30976184386663713No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39630.28233352972481207No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38210.27221711256081427No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36940.2631693310127317No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGTTGGTACCAAGCTAAGTAATTTTTATTGTTGAAACTGTATAAAATACT34620.24664110015324234No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34390.24500252554217225No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32430.23103902016087954No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30190.21508072829654495No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29030.20681661286680025No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGACCCGGGATCCCT28940.2061754314972511No Hit
GTAATAGACGGCTGTGTCTTCGATTTCCAGGCTGTTCATTTGCAGATATA28110.20026231442252002No Hit
ATTTTATACAGTTTCAACAATAAAAATTACTTAGCTTGGTACCAACAGAA27520.19605901433325332No Hit
CATTTCACTCTCACCATCAACAGCCTGCAGCCTGAAGATGTGGCAGTTTATCACTGTCAGCAATATTATACTACC27310.19456292447097195No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26980.19221192611595836No Hit
GTTTATCACTGTCAGCAATATTATACTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA26590.18943347351457868No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26160.1863700514156216No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25990.18515893105091763No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25030.17831966310905997No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24650.17561245288207464No Hit
GTGATAAACTGCCACATCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAATGTGTCCCAGACCCGCTGCCA23460.1671346103291469No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23010.16392870348140112No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGTTTCGCTTTCGGTAACGCCTGG22730.1619339169983593No Hit
GTCTATTACTGTGCCGCAGTCAAGGCATACTATGATAACGATGGTTATTTCTCAAACTGGTTCGACCCCTGGGGC22640.16129273562881014No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21970.15651949654438863No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21830.1555221033028677No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21770.1550946490565016No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20690.14740047262191172No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20450.1456906556364473No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20310.1446932623949264No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20230.1441233233997716No Hit
GAGTGAAATGTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGATCCCG19260.13721281308351957No Hit
GCCTGAAGATGTGGCAGTTTATCACTGTCAGCAATATTATACTACCCCGC19180.13664287408836476No Hit
GTATAAAATACTCTGACTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA18790.13386442148698507No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18780.13379317911259073No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG18650.13286702824546417No Hit
CTCCTGTGCAGCCTCTGGTTTCGCTTTCGGTAACGCCTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGC18190.12958987902332403No Hit
TCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAATGTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGATC18030.12845000103301443No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17940.12780881966346527No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17820.12695391117073304No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17690.12602776030360652No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17680.12595651792921214No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTATTTTATACAGTTTCAA17300.12324930770222682No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17270.12303558057904376No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC16840.1199721584800867No Hit
GTTGATGGTGAGAGTGAAATGTGTCCCAGACCCGCTGCCACTGAATCGGT16840.1199721584800867No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16820.11982967373129799No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGA16780.11954470423372059No Hit
CACCATCAACAGCCTGCAGCCTGAAGATGTGGCAGTTTATCACTGTCAGCAATATTATACTACCCCGCTCACTTT16690.11890352286417143No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16640.11854731099219967No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16300.11612507026279174No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16220.11555513126763695No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16000.11398779903096122No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTATTTTATA15700.1118505277991307No Hit
GGCCTGGAGTGGGTCGGCCGTATTAAGGGCAAAACTGATGGTGAGACAAT15670.11163680067594764No Hit
ATTCAGTGGCAGCGGGTCTGGGACACATTTCACTCTCACCATCAACAGCC15660.11156555830155329No Hit
GGACTGAACACAGAGGACTCCCCATGGAGTTTGGGCTGAGCTGGATTTTC15590.11106686168079286No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15530.11063940743442674No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15270.10878710570017362No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15200.10828840907941316No Hit
CTCCAGGCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAGGCGTTAC14900.10615113784758265No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14740.10501125985727303No Hit
GGGTTCCCTGGCCCCAGGGGTCGAACCAGTTTGAGAAATAACCATCGTTA14670.10451256323651258No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14650.10437007848772388No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14650.10437007848772388No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14510.10337268524620295No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14390.10251777675347076No Hit
GTCCTGGTTTCTGTTGGTACCAAGCTAAGTAATTTTTATTGTTGAAACTG14110.10052299027042892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3200.044.653931
GGTATCA9700.044.5443231
TGGTATC3350.040.6117552
TCTTGAG12350.036.43784370
GTATCAA22650.034.397561
GACAGGT1950.033.149681
AATGGTA3900.032.9675770
TATCAAC25400.030.396862
GCTTGTA450.00494948230.2418161
ACAGGTA2250.030.2418161
GCTTATA450.00494948230.2418161
GAGTTTA3150.030.2418161
AGGTACG450.00495638730.2331982
ATCAACG26050.030.0343743
TCAACGC26450.029.708784
CAACGCA26750.029.3755955
AACGCAG26900.029.338256
CTCTTGA12800.029.19224269
ACAGGTT1452.7284841E-1128.1561741
ACGCAGA28500.027.8105497
Produced by FastQC (version 0.11.5)