FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004689231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228985
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT48870.39764521129224517No Hit
GATTATTGCTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTG40950.3332017884677193No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGCAGGACGGTCAGCTTGGTCCCTC38770.31546357359935234No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37490.3050484749610451No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC37480.30496710700293334No Hit
GCAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG37250.3030956439663625No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37220.3028515400920272No Hit
GCCTTGGGCTGACGCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCATTCAGGCTGTCATCCCATG35740.29080908229148444No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT34570.2812890311924067No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30880.2512642546491617No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC28910.23523476690114198No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28780.2341769834456889No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28630.2329564640740123No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT28280.2301085855401002No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGCGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC27700.22538924396961724No Hit
GTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT26090.2122890027136214No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25950.21114985130005653No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25900.2107430115094977No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24950.20301305548887905No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24330.19796824208594896No Hit
ATACCACACCATTCAGGCTGTCATCCCATGCTGCACAGCAATAATCAGCC24310.19780550616972542No Hit
GCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGCGTCAGCCCAAGG24060.19577130721693103No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT24060.19577130721693103No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23990.19520173151014863No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23910.19455078784525442No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23360.1900755501491068No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23270.1893432385261008No Hit
CCCCTGAGGGCCGCTGATTATTATTAGAGATGAGGAGTTTGGGGGCCGCTCCTGGGAGCTGCTGGTACCAGTTTA23260.18926187056798904No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22200.18063686700814086No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC22160.18031139517569378No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT21890.17811446030667583No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG21820.1775448845998934No Hit
GTCTGAGGATGAGGCTGATTATTGCTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGAC21550.17534794973087547No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC20680.16826893737515106No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG20370.16574653067368603No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT20200.16436327538578585No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC20190.16428190742767407No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC20070.16330549193033275No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19850.16151539685187372No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT19300.15704015915572608No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA19190.15614511161649655No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19180.15606374365838477No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAC18170.14784557988909547No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18010.14654369255930708No Hit
GAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAGCGGCCCCCAAA17620.14337034219294784No Hit
CTCCTGGGAGCTGCTGGTACCAGTTTACAGTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGA17570.14296350240238898No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGCAGGACGGTCAGCTTGGTC17140.13946468020358263No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17040.13865100062246488No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16990.138244160831906No Hit
CCATTCAGGCTGTCATCCCATGCTGCACAGCAATAATCAGCCTCATCCTC16380.13328071538708772No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG16260.1323042998897464No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16210.13189746009918754No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16200.13181609214107576No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC16070.1307583086856227No Hit
GTCCTGCGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16000.13018873297884026No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15970.12994462910450494No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTGCTGTGCAG15710.12782906219359877No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15630.1271781185287046No Hit
ATCCCATGCTGCACAGCAATAATCAGCCTCATCCTCAGACTGGAGCCCAC15600.12693401465436926No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA15460.12579486324080438No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15230.12392340020423358No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT15210.12376066428801002No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC15120.12302835266500405No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTGCGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC15060.1225401449163334No Hit
GTTTGGGGGCCGCTCCTGGGAGCTGCTGGTACCAGTTTACAGTATTACTT14850.1208314177959861No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14630.11904132271752707No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14540.11830901109452108No Hit
GGACCAAGCTGACCGTCCTGCGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG14530.11822764313640932No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14300.11635618009983849No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14190.11546113256060897No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG14110.11481018889571476No Hit
GCTTGGTCCCTCCGCCGAATACCACACCATTCAGGCTGTCATCCCATGCTGCACAGCAATAATCAGCCTCATCCT14050.1143219811470441No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAGCGGCCCC14010.11399650931459701No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13990.11383377339837346No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13910.11318282973347925No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC13560.11033495119956711No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13420.10919579978600226No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13410.1091144318278905No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13250.10781254449810208No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13040.10610381737775482No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT12930.10520876983852528No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC12730.10358141067628979No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG12710.10341867476006622No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC12660.10301183496950737No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC12640.1028490990532838No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC12580.10236089130461316No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG12520.1018726835559425No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)