FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228985
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49930.4062702148520934No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC45300.3685968502463415No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43750.35598481673901633No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG40120.32644824794444194No Hit
GATTATTGCTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTG37490.3050484749610451No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36090.2936569608253966No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG34490.2806380875275125No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT33230.27038572480542883No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT32250.2624116649104749No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGCAGGACGGTCAGCTTGGTCCCTC31680.25777369129810374No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC31040.2525661419789501No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGCGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC30820.25077604690049105No Hit
GCCTTGGGCTGACGCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCATTCAGGCTGTCATCCCATG30770.25036920710993216No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28700.23352603978079473No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG27070.22026306260857537No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26970.21944938302745762No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC26620.21660150449354548No Hit
GTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT26400.21481140941508642No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25990.21147532313250364No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24610.2002465449130787No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24000.1952830994682604No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23930.19471352376147796No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23840.193981212138472No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG23670.19259795685057182No Hit
CCCCTGAGGGCCGCTGATTATTATTAGAGATGAGGAGTTTGGGGGCCGCTCCTGGGAGCTGCTGGTACCAGTTTA23360.1900755501491068No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23270.1893432385261008No Hit
GTCTGAGGATGAGGCTGATTATTGCTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGAC22620.18405432124883542No Hit
GCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGCGTCAGCCCAAGG22510.1831592737096059No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA22200.18063686700814086No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21830.17762625255800518No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT21800.17738214868366986No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC21690.17648710114444033No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG21500.1749411099403166No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT21440.17445290219164597No Hit
GAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAGCGGCCCCCAAA20890.1699776644954983No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20810.1693267208306041No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT20300.16517695496690357No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19820.16127129297753837No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19240.1565519514070554No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18830.15321586512447263No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18720.1523208175852431No Hit
CTCCTGGGAGCTGCTGGTACCAGTTTACAGTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGA18620.15150713800412535No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18580.15118166617167825No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18440.1500425147581134No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAGCGGCCCC18260.14857789151210143No Hit
ATACCACACCATTCAGGCTGTCATCCCATGCTGCACAGCAATAATCAGCC18100.14727600418231304No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17720.14418402177406558No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG17370.14133614324015345No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA17280.14060383161714748No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT16980.13816279287379424No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16560.13474533863309968No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTGCTGTGCAG16080.13083967664373447No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC16000.13018873297884026No Hit
GTTTGGGGGCCGCTCCTGGGAGCTGCTGGTACCAGTTTACAGTATTACTT15760.12823590198415766No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15580.1267712787381457No Hit
GCAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG15530.12636443894758684No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15410.12538802345024552No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC15410.12538802345024552No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG15320.12465571182723954No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15300.124492975911016No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTGCGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC15260.12416750407856891No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGCAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAC14910.12131962554465676No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGCAGGACGGTCAGCTTGGTC14790.12034321004731546No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC14680.11944816250808593No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14530.11822764313640932No Hit
GTCCTGCGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14080.11456608502137942No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC13970.1136710374821499No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13850.11269462198480862No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13650.1110672628225731No Hit
GGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTGCGTCAGCCCAAGGCTGCCCC13590.11057905507390245No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13360.10870759203733163No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACT13210.10748707266565499No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC13090.10651065716831369No Hit
GGACCAAGCTGACCGTCCTGCGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG12790.10406961842496043No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12750.10374414659251334No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC12680.10317457088573091No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG12640.1028490990532838No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA150.002238874869.8897650
TGATAGT400.005261458556.32716870
ACTATCG200.00781546650.9684648
CGCAATT306.816709E-445.2868612
GGTATCA13300.044.4681781
TGGTATC3600.040.5975652
ACGCAAT350.001454566638.82205211
GTATCAA31300.036.9220921
TATCAAC35250.032.783392
ATCAACG36600.031.848873
CGGAGTC553.0932552E-431.6409147
TCAACGC37000.031.592554
CAACGCA37300.031.3371775
GTAATAG553.5577052E-430.8950083
AACGCAG38200.030.6878176
GACGCAA450.0049819830.20107510
CGCAGAG39250.029.7802988
ACGCAGA39300.029.7424097
AGAGTAC40100.029.31016511
CAGAGTA40350.029.13449310
Produced by FastQC (version 0.11.5)