FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397321
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46110.32998859961311683No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36900.26407675831108246No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA34030.2435374548868871No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31220.22342754456563668No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG29290.209615399754244No Hit
GTAGTACACGGCCGTGTCCTCATATCTGAGTCTGCTCAACTCCAGGAAGGCTGTACCTATGGAATTGTTCCTGGT28700.20539303424195302No Hit
GTCTGGGGCTGAGGTGAGGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA28670.20517833769048058No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28610.2047489445875357No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27630.1977355239061032No Hit
CTCCTGTAGGACCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTTCCAGCACAAACCTGGCCAGGCTCCCAGGCT26870.1922965446021351No Hit
CAGTAGTACACGGCCGTGTCCTCATATCTGAGTCTGCTCAACTCCAGGAA26190.1874300894354268No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24380.17447673082992382No Hit
GGGTATGCACAGAAGTTTCAGGGCAGAGTCACTATGACCAGGAACAATTC24240.1734748135897192No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23790.1702543653176328No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23010.16467225497934976No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGTAGGACCAGTCAGAGTGTTAGCAG22700.16245372394746804No Hit
GTGGTAACACAGGGTATGCACAGAAGTTTCAGGGCAGAGTCACTATGACC22620.16188119981020824No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22390.16023519291558633No Hit
TAGTAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCGGTGGGTCTGGGACAGAATTCACTCTCACCATCAGCAG21840.1562990894719252No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21810.15608439292045279No Hit
GTGTTACCACTGTCAGGGTTCATCCATCCCATCCACTCAAGTCCTTGTCC21690.15522560671456306No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21290.1523629860282641No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21200.1517188963738468No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20920.14971506189343753No Hit
GTCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT20810.14892784120470529No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20810.14892784120470529No Hit
GTACTACTGCGCGCGAGGGGCATGGCGACCCCCGCTGAACCGCTACGCTA20300.14527799982967407No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20160.14427608258946942No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19810.1417712894889578No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCGATTTATTACTGTCTGCAGAATGATAAGTGG19750.14134189638601297No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC19700.14098406880022557No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19630.14048311018012324No Hit
CATCTATGGTGCATATAGTAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCGGTGGGTCTGGGACAGAATTCA19570.1400537170771784No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCCCAGGGCCGAAAGTGAATATCGGGGGCCACTTATCATT19140.136976399839407No Hit
GTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGTAGGACCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTT19080.13654700673646214No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCCCAGGGCCGAAAGTGAATATCGGGGG18980.13583135156488738No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18750.13418534467026544No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18680.13368438605016314No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18410.1317521170869113No Hit
GTGTACTACTGCGCGCGAGGGGCATGGCGACCCCCGCTGAACCGCTACGC18280.13082176536386414No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18240.13053550329523425No Hit
ACTATATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGCT18120.12967671708934453No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT18100.12953358605502957No Hit
GTCCCAGACCCACCGCCACTGAACCTGGCTGGGACACCAGTGGCCCTACT17660.12638470330010068No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17570.1257406136456834No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17380.12438086881969139No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17210.12316425502801433No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17090.12230546882212462No Hit
GCATATAGTAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCGGTGGGTC16880.12080259296181764No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16850.12058789641034523No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGCTGGAACCAGGC16720.11965754468729806No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16330.11686648951815652No Hit
GTGCATACCCTGTGTTACCACTGTCAGGGTTCATCCATCCCATCCACTCAAGTCCTTGTCCGGTGGCCAGTCGCA16250.11629396538089673No Hit
CAGTAATAAATCGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGACCCACCGCCA16180.11579300676079442No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16010.11457639296911734No Hit
GTCAGAGTGTTAGCAGCAACTTAGCCTGGTTCCAGCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG15950.1141469998661725No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15870.11357447572891267No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15820.11321664814312532No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15700.1123578619372356No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT15510.11099811711124359No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT15140.10835019297641701No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14950.106990448150425No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14920.10677575159895257No Hit
ATTTATTACTGTCTGCAGAATGATAAGTGGCCCCCGATATTCACTTTCGGCCCTGGGACCAAAGTGGAGATCAAA14850.10627479297885024No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14770.10570226884159045No Hit
GTAATAAATCGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGACCCACCGCCA14670.1049866136700157No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTGG14620.10462878608422832No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14520.10391313091265358No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14290.10226712401803166No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14200.10162303436361438No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC14200.10162303436361438No Hit
GTTCAGTGGCGGTGGGTCTGGGACAGAATTCACTCTCACCATCAGCAGCC14090.10083581367488215No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)