FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703313
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC81700.4796534753154587No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT72520.42575850709763857No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC58320.3423915627955637No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC51750.30381967377692765No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG49820.29248881444573016No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG48040.282038591850118No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA44710.26248845632012435No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG43540.25561948978255905No Hit
CCCCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG42100.24716537712094017No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42080.24704795888952882No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT38560.22638235016112715No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37620.22086369328479263No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35920.21088314361482594No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32760.192331063051829No Hit
CTACAAACGCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACG32230.1892194799194276No Hit
GAGGTAGACAGCCGTGTCCTCGGCTCTGAGACTGTTCATTTGGAGATGGA31990.18781046114249114No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31260.18352469569597601No Hit
CATTAGTGCTGATGGATTTAGCACAGACTATGCGGACTCCGTGAAGGGCCGATTCACCGTCTCCAGAGACACCGC31180.18305502277033053No Hit
GGTCTAGTCAGAGCCTCCTGCAGACTAATGGATACAACTATTTAAATTGGTACCTACAGAGGCCAGGGCGGTCTC30640.17988473052222345No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30100.1767144382741164No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG29630.1739551098359491No Hit
GGCCAGGGCGGTCTCCACAACTCCTGATCTTTTTGGGGTCCAATCGGCCC29000.17025643554649086No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28710.16855387119102597No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT28510.16737968887691224No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26400.15499206546301236No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26270.15422884695883846No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26250.15411142872742709No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG26070.15305466464472472No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT26030.15281982818190196No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC26030.15281982818190196No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25310.14859277185109254No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24960.14653795280139353No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTCAA23950.1406083321151192No Hit
GCGTTTGTAGAGGTTGCATGCAGTAATAAATCCCAACATCCTCAGCCTCAACTCTGCTGATTTTGAGTGTAAAAT23940.1405496229994135No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCT23710.1391993133381827No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT23570.1383773857183031No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC23550.13825996748689173No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT23420.1374967489827178No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC23330.13696836694136663No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG23290.13673353047854386No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC22740.1335045291147311No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA22620.13280001972626287No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGAA22620.13280001972626287No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC22590.13262389237914582No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG22540.1323303468006174No Hit
GAGTTGAGGCTGAGGATGTTGGGATTTATTACTGCATGCAACCTCTACAAACGCCGCTCACTTTCGGCGGAGGGA22160.1300994004038013No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22020.12927747278392168No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA21760.12775103577557384No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGATGAGTCAGTCTCCACTCTCCCTGTCCGTCACCC21670.12722265373422265No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG21420.1257549258415805No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT21380.12552008937875775No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGATGAGTCAGTCTCCACTCTCC21120.12399365237040991No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20840.12234979713065068No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20820.12223237889923931No Hit
GTCAGGGACCCCGGAGGGCCGATTGGACCCCAAAAAGATCAGGAGTTGTGGAGACCGCCCTGGCCTCTGTAGGTA20760.12188012420500519No Hit
GATTTTACACTCAAAATCAGCAGAGTTGAGGCTGAGGATGTTGGGATTTATTACTGCATGCAACCTCTACAAACG20750.1218214150892995No Hit
CTCCAGGGGTGACGGACAGGGAGAGTGGAGACTGACTCATCACAATGTCC20550.12064723277518577No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT20300.11917950488254361No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG19970.11724210406425595No Hit
CTGTAGGTACCAATTTAAATAGTTGTATCCATTAGTCTGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGC19820.11636146732867067No Hit
GTAATAAATCCCAACATCCTCAGCCTCAACTCTGCTGATTTTGAGTGTAAAATCTGTGCCTGATCCACTGCCA19760.11600921263443655No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19720.1157743761716138No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19250.11301504773344653No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG19110.11219312011356691No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18890.11090151956804181No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC18510.10867057317122573No Hit
GTACCTACAGAGGCCAGGGCGGTCTCCACAACTCCTGATCTTTTTGGGGTCCAATCGGCCCTCCGGGGTCCCTGA18490.10855315493981435No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18450.10831831847699161No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG18440.10825960936128591No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18410.10808348201416887No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTCAAAATCAGCAGAG18330.10761380908852337No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT18210.10690929970005514No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18140.10649833589011531No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18060.10602866296446983No Hit
CTAATGGATACAACTATTTAAATTGGTACCTACAGAGGCCAGGGCGGTCTCCACAACTCCTGATCTTTTTGGGGT17960.10544157180741297No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17940.10532415357600158No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17810.10456093507182768No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17800.10450222595612198No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG17720.10403255303047648No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG17650.10362158922053669No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17450.10244740690642297No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA17410.10221257044360021No Hit
GACTAGACCTGCAGGAGATGGAGGCCGACTCTCCAGGGGTGACGGACAGG17230.10115580636089784No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)