FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703313
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT72920.42810687172586603No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC64870.3808460335827884No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC58610.3440941271510286No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC54590.3204930626373427No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA41680.24469959426130136No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG41260.2422338114016625No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39750.23336873493010388No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG39620.23260551642592994No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38950.22867200567364895No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT38740.22743911424382954No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36470.21411214497863867No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35370.20765414225101317No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34640.20336837680449807No Hit
CCCCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG32400.19021753488642426No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31610.18557951474567505No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA30880.18129374929915992No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT30330.17806474793534716No Hit
GGTCTAGTCAGAGCCTCCTGCAGACTAATGGATACAACTATTTAAATTGGTACCTACAGAGGCCAGGGCGGTCTC30100.1767144382741164No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29560.17354414602600932No Hit
GAGGTAGACAGCCGTGTCCTCGGCTCTGAGACTGTTCATTTGGAGATGGA28870.16949321704231693No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28850.16937579881090556No Hit
CTACAAACGCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACG28550.16761452533973498No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT28370.16655776125703262No Hit
CATTAGTGCTGATGGATTTAGCACAGACTATGCGGACTCCGTGAAGGGCCGATTCACCGTCTCCAGAGACACCGC28240.16579454275285868No Hit
GGCCAGGGCGGTCTCCACAACTCCTGATCTTTTTGGGGTCCAATCGGCCC27310.16033459499222985No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27050.158808157983882No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG26070.15305466464472472No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25980.15252628260337353No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25450.14941469947097216No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25360.148886317429621No Hit
GCGTTTGTAGAGGTTGCATGCAGTAATAAATCCCAACATCCTCAGCCTCAACTCTGCTGATTTTGAGTGTAAAAT25350.14882760831391528No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA25070.14718375307415607No Hit
GAGTTGAGGCTGAGGATGTTGGGATTTATTACTGCATGCAACCTCTACAAACGCCGCTCACTTTCGGCGGAGGGA23210.13626385755289838No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT23150.13591160285866427No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT22820.1339742020403766No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22820.1339742020403766No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC22670.13309356530479133No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTCAA22160.1300994004038013No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22160.1300994004038013No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22070.12957101836245014No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGAA21780.1278684540069852No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21770.12780974489127953No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21520.12634201699863737No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG21490.12616588965152029No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC21160.12422848883323265No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG21140.12411107060182128No Hit
GTAATAAATCCCAACATCCTCAGCCTCAACTCTGCTGATTTTGAGTGTAAAATCTGTGCCTGATCCACTGCCA21010.12334785209764734No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC20870.12252592447776774No Hit
GATTTTACACTCAAAATCAGCAGAGTTGAGGCTGAGGATGTTGGGATTTATTACTGCATGCAACCTCTACAAACG20650.12123432393224264No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC20560.12070594189089147No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGATGAGTCAGTCTCCACTCTCC20440.12000143250242322No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGATGAGTCAGTCTCCACTCTCCCTGTCCGTCACCC20220.11870983195689812No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCT20120.11812274079984125No Hit
CTCCAGGGGTGACGGACAGGGAGAGTGGAGACTGACTCATCACAATGTCC19850.11653759467578771No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19850.11653759467578771No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19520.11460019385750005No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC19350.11360213889050338No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG19280.11319117508056357No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19270.1131324659648579No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19100.11213441099786121No Hit
CTGTAGGTACCAATTTAAATAGTTGTATCCATTAGTCTGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGC19000.11154731984080435No Hit
GTCAGGGACCCCGGAGGGCCGATTGGACCCCAAAAAGATCAGGAGTTGTGGAGACCGCCCTGGCCTCTGTAGGTA18690.10972733725392808No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG18590.10914024609687122No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT18340.10767251820422905No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTCAAAATCAGCAGAG18240.10708542704717218No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18040.10591124473305845No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17870.1049131897660618No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA17840.10473706241894473No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17760.10426738949329924No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17550.10303449806347983No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17470.10256482513783434No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17370.10197773398077745No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17350.10186031574936608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)