FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1981638
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT95930.4840944713413853No Hit
CCCTAACTGTGTCTCTGGGCGAGAGGACCACCATCAACTGCAAGTCCAGT91630.46239525079757254No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC83820.4229834106935777No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC65310.3295758357480024No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG63360.31973549154790126No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC59610.30081175270155297No Hit
CTCCCTAACTGTGTCTCTGGGCGAGAGGACCACCATCAACTGCAAGTCCAGTCAGAGTCTTTTGTTCAACTCCAA55930.2822412569803365No Hit
GTTTATTACTGTCAGCAATATTATCTTCCTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA52410.26447817411656416No Hit
ATATTATCTTCCTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT52180.2633175181339882No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG51930.26205593554423157No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT50700.25584894920262935No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC50220.25342671063029676No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT48400.24424238937686907No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG46290.2335946323193237No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT45420.2292043249069709No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA45260.22839691204952672No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG45060.2273876459777215No Hit
GTCCCTGACCGATTCACTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC42590.2149232099909267No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC41250.20816112730983158No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC40960.206697691505714No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG40660.20518379239800608No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT40240.2030643336472151No Hit
ACTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCCAAGTAGTTCTTATTGTTGGAGTTGAACAAAAGACT40240.2030643336472151No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA38960.19660503078766153No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG38870.19615086105534918No Hit
GTCTCCAGACTCCCTAACTGTGTCTCTGGGCGAGAGGACCACCATCAACTGCAAGTCCAGTCAGAGTCTTTTGTT38130.19241657658966976No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATCTTCCT37780.19065036096401058No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC37550.18948970498143455No Hit
CTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT36490.18414059480086675No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGTAACTTAGGAGGCTGTCCTGGTTTTTGCTGGTA35870.18101186997827048No Hit
AACCAGGACAGCCTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAA34530.17424978729717536No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG34400.17359376435050194No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT32820.16562056238324052No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32660.16481314952579632No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA32460.1638038834539911No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG32420.16360203023963005No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG32420.16360203023963005No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31750.16022098889908248No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT30040.15159176398514765No Hit
GTAAATGAGTAACTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCCAAGTAGTTCTTATTGTTGGAGTTGAA29830.15053203460975215No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA29620.14947230523435662No Hit
GTCCTGGTTTTTGCTGGTACCAAGCCAAGTAGTTCTTATTGTTGGAGTTG29530.14901813550204426No Hit
CAGTTAGGGAGTCTGGAGACTGGGTCATCACGATGTCCCCGTAGGCACCA29050.14659589692971167No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA28410.1433662454999349No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATCTTCCTCCTCTCACTTT28370.14316439228557384No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT27710.13983381424861654No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC27230.13741157567628395No Hit
AGATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC26860.13554443344344425No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTAACTGTGTCTCTGGGCGAGAGGA26270.1325670985316188No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC25920.13080088290595962No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATCTTCCTCCTC25650.12943837370902253No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC25310.12772262138695362No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT25200.12716752504746073No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG25130.12681428192232888No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT25080.12656196540437759No Hit
ACCATCAACTGCAAGTCCAGTCAGAGTCTTTTGTTCAACTCCAACAATAA24940.1258554791541139No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT24880.12555269933257235No Hit
CTACTTGGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAATC24690.12459389656435736No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA24630.12429111674281579No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA24530.12378648370691317No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA24520.12373602040332292No Hit
TTATTACTGTCAGCAATATTATCTTCCTCCTCTCACTTTCGGCGGAGGGA24150.12186887817048321No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTAACTGTGTCTCTGGGCGAGAGGACCACCATCAACTG23960.12091007540226822No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA23920.12070822218790717No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT23640.11929524968737984No Hit
CTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCCAAGTAGTTCTTAT23590.11904293316942853No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG23520.11868969004429669No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG23490.11853830013352591No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC23110.11662069459709595No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC22950.11581328173965175No Hit
CTGTGTCTCTGGGCGAGAGGACCACCATCAACTGCAAGTCCAGTCAGAGT22840.11525818540015886No Hit
CAATAAGAACTACTTGGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAAGTTACTCATTTACTGGGCATCTAC22780.11495540557861729No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT22690.11450123584630494No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT22330.11268455691705549No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA22280.11243224039910417No Hit
GCCTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCACTGGCAGCGGGT22260.11233131379192365No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC22170.1118771440596113No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC22000.11101926789857683No Hit
CTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAC21950.11076695138062553No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT21700.10950536879086897No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21660.10930351557650793No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC21590.10895027245137609No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG21560.1087988825406053No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC21180.10688127700417534No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21160.1067803503969948No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG21090.10642710727186298No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG20890.10541784120005773No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC20840.10516552468210641No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT20800.10496367146774538No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCACTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC20740.1046608916462038No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA20640.10415625861030119No Hit
CTTTTGTTCAACTCCAACAATAAGAACTACTTGGCTTGGTACCAGCAAAA20640.10415625861030119No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT20550.1037020888779888No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC20310.10249096959182252No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20280.10233957968105174No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGAGGAGGAAGATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGC19870.10027058423385099No Hit
GAACTACTTGGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAAGTTAC19870.10027058423385099No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT200.007233895451.97633445
GTGGTAT3500.045.841611
TGGTATC3500.045.8173182
GGTATCA13600.044.93081
CTTATAC5900.039.923361
TCGTATG5600.039.4071240
GTATGCC5900.039.23970442
CGTATGC5750.039.01559441
TATACAC6050.038.348943
GCCGTCT5450.038.1937746
ATCTCGT4600.038.1077237
GTATCAA33400.037.6124271
ACGAGAC6250.037.13114522
GACAGGT3600.036.982151
TCTCCGA6300.036.82715611
CGAGACA6400.036.79784823
ATGCCGT5900.036.3745844
ATCTCCG6550.035.42064710
ATACACA7050.035.329184
CCCACGA6600.035.15674619
Produced by FastQC (version 0.11.5)