FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1981638
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT97680.49292554946968115No Hit
CCCTAACTGTGTCTCTGGGCGAGAGGACCACCATCAACTGCAAGTCCAGT74050.3736807630858916No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC67910.34269629468147056No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC65550.33078695503416866No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC64630.3261443311038646No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG58090.29314133055583314No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57330.2893061194829732No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT54480.2749240779597485No Hit
GTTTATTACTGTCAGCAATATTATCTTCCTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA53680.2708870136725275No Hit
ATATTATCTTCCTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT52870.2667994860817162No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA52120.26301473831244654No Hit
CTCCCTAACTGTGTCTCTGGGCGAGAGGACCACCATCAACTGCAAGTCCAGTCAGAGTCTTTTGTTCAACTCCAA50420.254435976702102No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT48290.24368729303737616No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC47420.23929698562502336No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT47080.23758123330295441No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG43560.21981815043918213No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG43320.21860703115301586No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA42820.21608386597350274No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG40330.20351850337952745No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC40200.20286248043285404No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC40030.2020046042718196No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC39900.20134858132514616No Hit
GTCCCTGACCGATTCACTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC38830.1959490078409881No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT38470.19413232891173868No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38000.19176055364299635No Hit
GTCCCAGACCCGCTGCCAGTGAATCGGTCAGGGACCCCGGATTCCCGGGT37920.19135684721427426No Hit
ACTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCCAAGTAGTTCTTATTGTTGGAGTTGAACAAAAGACT37700.1902466545352885No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATCTTCCT37240.1879253425701364No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT36960.18651237006960908No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCAGTGAATCGGTCAGGGACCCCG36850.1859572737301162No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA35850.18091094337108998No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG34960.17641970935155665No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGTAACTTAGGAGGCTGTCCTGGTTTTTGCTGGTA33360.1683455807771147No Hit
AGATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC32330.1631478605073177No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT32290.16294600729295663No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG32170.1623404476498735No Hit
CTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT31910.16102840175652666No Hit
GTCTCCAGACTCCCTAACTGTGTCTCTGGGCGAGAGGACCACCATCAACTGCAAGTCCAGTCAGAGTCTTTTGTT31750.16022098889908248No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT30550.154165392468251No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG30460.15371122273593865No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA30080.15179361719950868No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29860.1506834245205229No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCAGTGAATCGGTCAGGGACC29490.1488162822876832No Hit
GTCCTGGTTTTTGCTGGTACCAAGCCAAGTAGTTCTTATTGTTGGAGTTG29280.1477565529122877No Hit
GTAAATGAGTAACTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCCAAGTAGTTCTTATTGTTGGAGTTGAA28310.14286161246403228No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA28250.1425588326424907No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT27880.14069169040965102No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT27370.13811806192654763No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATCTTCCTCCTC27130.13690694264038133No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT26950.13599860317575663No Hit
CAGTTAGGGAGTCTGGAGACTGGGTCATCACGATGTCCCCGTAGGCACCA26840.13544350683626374No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA26660.13453516737163904No Hit
AACCAGGACAGCCTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAA26510.13377821781778507No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATCTTCCTCCTCTCACTTT26000.1312045893346817No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25790.1301448599592862No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA25660.1294888370126128No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA25650.12943837370902253No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT25150.12691520852950944No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC24740.12484621308230869No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTAACTGTGTCTCTGGGCGAGAGGA24610.12419019013563527No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC24400.12313046076023976No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC24120.12171748825971242No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC23640.11929524968737984No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC23120.11667115790068619No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC23010.11611606156119332No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG22990.11601513495401279No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG22930.11571235513247122No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT22840.11525818540015886No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC22760.11485447897143675No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA22740.11475355236425623No Hit
TTATTACTGTCAGCAATATTATCTTCCTCCTCTCACTTTCGGCGGAGGGA21820.11011092843395212No Hit
ACCATCAACTGCAAGTCCAGTCAGAGTCTTTTGTTCAACTCCAACAATAA21620.10910166236214687No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT21620.10910166236214687No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGAGGAGGAAGATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGC21560.1087988825406053No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA21340.10768868986161953No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC21090.10642710727186298No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20910.10551876780723825No Hit
CTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCCAAGTAGTTCTTAT20820.1050645980749259No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG20820.1050645980749259No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC20700.10445903843184276No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT20620.10405533200312064No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCAGTGAATCGGT20620.10405533200312064No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA20550.1037020888779888No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGAGGAGGAAGATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGC20480.103348845752857No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTAACTGTGTCTCTGGGCGAGAGGACCACCATCAACTG20460.10324791914567645No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT19840.10011919432308021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3650.052.2377551
TGGTATC3850.048.6372872
GGTATCA14500.043.6751371
GTATCAA36100.037.6317981
GTTACTA400.002660334.33038736
GTACGGT400.002765520234.0598615
GACAGGT3600.032.1562731
TATCAAC42650.031.6113852
ATCAACG43400.030.9858763
TCAACGC43850.030.511074
CAACGCA44000.030.3289185
AACGCAG44050.030.2944936
ACGCAGA47100.028.4050247
CGCAGAG47950.027.972498
AGAGTAC48200.027.82318911
GCAGAGT49200.027.3310019
CGTAATC500.00814745327.30858427
GGCACTA500.00819485127.27613425
CAGAGTA49300.027.27418310
GCTTCGA500.00825059227.2382551
Produced by FastQC (version 0.11.5)