FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276837
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTATTACTGTGCGAGAGAGATCTTCCCGATATCACTGGCTGGCAACTT48260.37796523753619293No Hit
GTAATAAGAAGCGCTGTCCTCAGGTCTCAGGCTGTTCATTTGCAGAAAGA32920.2578246087793508No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31630.24772151809510531No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28120.22023171321006516No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG25460.19939898358208602No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23050.1805242172650072No Hit
CTCCAGTCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATTGCATAACTGA22850.17895784661628697No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22840.17887952808385094No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21720.17010785245101764No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21000.16446891811562478No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20060.15710697606663968No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19440.15225122705560695No Hit
GTTCAGTGGCAGTGGATCTGGGACAACCTTCACTCTCACCATCAGCAGCC18920.14817866336893434No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18450.14449769234444176No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18370.1438711440849537No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT18290.1432445958254656No Hit
GAGTGAAGGTTGTCCCAGATCCACTGCCACTGAACCGAGCCGGGACCCCT18090.14167822517674536No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18090.14167822517674536No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18040.14128663251456527No Hit
CTCCTGTGCAGTCTCTGGATTCACATTTATCAGTTATGCAATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGA18010.14105167691725726No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17860.1398768989307171No Hit
GATCTATGCTGCATCGACTTTGCAATCAGGGGTCCCGGCTCGGTTCAGTGGCAGTGGATCTGGGACAACCTTCA17750.13901539507392094No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17000.13314150514122006No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16850.13196672715467989No Hit
ACTTATTACTGTGAAAATTATGACAGAAGTCCCGGATTCACATTCGGCCC16800.13157513449249983No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16760.13126186036275578No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16720.13094858623301173No Hit
GAACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCATCACTGAACACAGA16700.13079194916813972No Hit
GTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAGGTTGTCCCAGATCCACTGCCA16360.12812911906531532No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16300.12765920787069923No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16070.12585788162467096No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16040.1256229260273629No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15680.12280345885966651No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCTCCATCACTT15560.12186363647043437No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15530.12162868087312632No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15400.12061053995145816No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14950.11708620599183764No Hit
CCTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTGAAAATTATGACAGAAGTC14940.11700788745940163No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14780.11575479094042544No Hit
GCCTGAGACCTGAGGACAGCGCTTCTTATTACTGTGCGAGAGAGATCTTC14590.1142667388241412No Hit
GTCCAGAGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT14520.11371850909708914No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14170.11097736046182871No Hit
GCATAGATCAGGAGCTTAGGAGCCTTCCCTGGTTTCTGCTGATACCAGGC14150.1108207233969567No Hit
CTCTGGACCCTGGGCCGAATGTGAATCCGGGACTTCTGTCATAATTTTCA14040.10995921954016057No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG13950.10925435274823647No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13920.10901939715092843No Hit
GGACTGGAGTGGGTGGCCTCTATATCATATGATGGAAGAATTACATATTA13810.1081578932941323No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG13550.106121611450796No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13470.1054950631913079No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG13280.10400701107502368No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13200.10338046281553558No Hit
CCTGAGGACAGCGCTTCTTATTACTGTGCGAGAGAGATCTTCCCGATATC13100.10259727749117545No Hit
CCTTGGGGCTGGTCGGGGATGCTGCGGAGACGGTGACCAGGGTTCCCTGG12980.10165745510194331No Hit
GGGTTCCCTGGCCCCAGAAGTCAAAGTTGCCAGCCAGTGATATCGGGAAGATCTCTCTCGCACAGTAATAAGAAG12850.10063931418027516No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATGCCAGATGTGACAT12810.10032604005053111No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT352.27701E-549.27104639
GCGTATG506.8819645E-848.3568340
TCCGATA306.843044E-445.2515648
ATCGCGT503.951078E-641.23343337
AATCGCG503.983494E-641.1844536
CGTATGC3800.040.05183441
GGCGAAT4650.039.67992432
AATGGTA3800.039.3528970
GCCGTCT3050.038.8573646
CCGATAA350.001461049238.7870569
GCGAATC4000.038.4793733
GTCGTAT451.003376E-438.32192639
CTTATAC5200.037.870991
TCGTATG3650.037.852740
CGAATCG557.775661E-637.36198834
ACTAAGG5100.036.71267327
CTTGAAA3600.036.52935457
TAAGGCG5350.036.31968329
GACTAAG5200.035.97980526
GTATCAA30150.035.9240571
Produced by FastQC (version 0.11.5)