FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276837
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46350.3630063978409147No Hit
TCTTATTACTGTGCGAGAGAGATCTTCCCGATATCACTGGCTGGCAACTT45780.35854224149206204No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30960.24247417642189253No Hit
GTAATAAGAAGCGCTGTCCTCAGGTCTCAGGCTGTTCATTTGCAGAAAGA29070.2276719737914863No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC24260.19000075968976463No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24020.18812111491130035No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22550.1766082906432066No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22490.17613837944859054No Hit
CTCCAGTCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATTGCATAACTGA21560.16885475593204144No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20020.15679370193689562No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19990.1565587463395876No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19690.15420919036650724No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19380.15178131586099086No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19200.15037158227714265No Hit
GTTCAGTGGCAGTGGATCTGGGACAACCTTCACTCTCACCATCAGCAGCC18760.14692556684995814No Hit
CTCCTGTGCAGTCTCTGGATTCACATTTATCAGTTATGCAATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGA18380.1439494626173897No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17900.1401901730604611No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17890.1401118545280251No Hit
GAGTGAAGGTTGTCCCAGATCCACTGCCACTGAACCGAGCCGGGACCCCT17840.13972026186584505No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17050.1335330978034001No Hit
GATCTATGCTGCATCGACTTTGCAATCAGGGGTCCCGGCTCGGTTCAGTGGCAGTGGATCTGGGACAACCTTCA17030.1333764607385281No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16360.12812911906531532No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16140.12640611135172306No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16110.126171155754415No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCTCCATCACTT15740.12327337005428257No Hit
GTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAGGTTGTCCCAGATCCACTGCCA15200.11904416930273794No Hit
CCTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTGAAAATTATGACAGAAGTC15150.1186525766405579No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15070.11802602838106979No Hit
GAACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCATCACTGAACACAGA15050.11786939131619777No Hit
ACTTATTACTGTGAAAATTATGACAGAAGTCCCGGATTCACATTCGGCCC14940.11700788745940163No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14820.1160680650701695No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14540.11387514616196116No Hit
CTCTGGACCCTGGGCCGAATGTGAATCCGGGACTTCTGTCATAATTTTCA14520.11371850909708914No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG14320.1121521384483689No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG14230.1114472716564448No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14020.10980258247528854No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13970.1094109898131085No Hit
GGGTTCCCTGGCCCCAGAAGTCAAAGTTGCCAGCCAGTGATATCGGGAAGATCTCTCTCGCACAGTAATAAGAAG13920.10901939715092843No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13760.10776630063195224No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13740.10760966356708021No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13590.10643488558054004No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG13450.10533842612643587No Hit
GTCCAGAGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT13360.10463355933451177No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13350.10455524080207575No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13300.1041636481398957No Hit
GCATAGATCAGGAGCTTAGGAGCCTTCCCTGGTTTCTGCTGATACCAGGC13290.1040853296074597No Hit
GCCTGAGACCTGAGGACAGCGCTTCTTATTACTGTGCGAGAGAGATCTTC13180.10322382575066356No Hit
CCTTGGGGCTGGTCGGGGATGCTGCGGAGACGGTGACCAGGGTTCCCTGG12930.10126586243976327No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12890.10095258831001921No Hit
GGACTGGAGTGGGTGGCCTCTATATCATATGATGGAAGAATTACATATTA12890.10095258831001921No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12860.10071763271271117No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12860.10071763271271117No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAT200.0075202251.46746436
TTGTCGC350.001377632839.25375437
TTTGTCG350.001384631639.21330636
CCTAACG451.10668065E-437.6894072
CTAACGC451.10668065E-437.6894073
GTATCAA28050.036.8846661
GACAGGT2800.033.92181
GTGGTAT3950.033.4924131
TGCACAG12750.031.44629970
TATCAAC32800.031.3350682
GTTCTAT652.6060026E-531.3124311
ATCAACG32850.031.0808563
TCAACGC33050.030.8939844
AACGCAG33700.030.497036
CAACGCA34100.030.0398255
CGCAGAG35350.029.1695048
AGAGTAC36150.028.98858511
ACGCAGA35700.028.8835267
GCAGAGT36550.028.4902239
CAGAGTA37000.028.32595610
Produced by FastQC (version 0.11.5)