FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079319
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATACTAGGTCACGGGGGACGTTCGGCCAAGGGA37600.34836781340826944No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32090.2973171045816853No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29880.27684123044252906No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26820.248490020096005No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25820.23922491867557227No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22110.204851392405767No Hit
GTATACTAGGTCACGGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT19650.18205924291150255No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19370.17946501451378138No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18890.17501776583197368No Hit
CAGTAATACACTGCAAAATCTGCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18850.17464716177515638No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17710.16408494615586308No Hit
ATACTAGGTCACGGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA17360.16084216065871165No Hit
GTGTATTACTGTCAGCAGTATACTAGGTCACGGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA17200.1593597444314424No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16310.1511138041672573No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15960.14787101867010588No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15830.1466665554854496No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15800.14638860244283664No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15600.14453558215875012No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15120.1400883334769424No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14320.13267625234059624No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13570.12572742627527173No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13300.1232258488917549No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGCAGATT13100.12137282860766835No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12800.11859329818153855No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12770.11831534513892555No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG12640.11711088195426932No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12210.11312688834348326No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAG11870.10997675386053613No Hit
ACCTAGTATACTGCTGACAGTAATACACTGCAAAATCTGCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT11720.10858698864747124No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11650.10793843154804095No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11410.1057148072071371No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA11390.10552950517872844No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11310.10478829706509384No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10940.10136020953953372No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10930.1012675585253294No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10840.10043369939749046No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGT451.3199335E-463.1442170
GTTAACG200.007628427451.2812832
GCGGTTG752.8751787E-547.3581670
GTATCAA30200.038.68571
GGTATCA14850.037.5073471
ATCAACG37800.030.807783
TATCAAC38000.030.5591162
TCAACGC38350.030.2746054
AACGCAG38500.030.0670816
CAACGCA38900.029.757915
TAACGGC1850.029.6617236
TTAACGG1850.029.6381335
GACTGAG4950.028.70191470
ACGCAGA40900.028.3027557
CGCAGAG41450.027.845318
CGGTACC2000.027.81078145
CAGTTCG9800.027.5450570
CCGTGCG650.00827480227.22005869
AGAGTAC42350.027.17088111
TCTCGCG7150.026.82524970
Produced by FastQC (version 0.11.5)