FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004689247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618857
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61250.37835336907460015No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53930.3331362807215214No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAATCAAAGCCTCGTA46350.28631312092420763No Hit
GTGTGTACCTTGCATGCAGTAATAAATCCCAACATCCTCAGCCTCCACCC44500.2748853048786891No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41470.25616839535548847No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40710.2514737249800322No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36690.22664138957301355No Hit
GGTACACACTGGCCTCTGTCGATCACCTTCGGCCAAGGGACACGACTGGA33690.2081097959856862No Hit
GTACACACTGGCCTCTGTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT32470.20057361459350642No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32460.200511842614882No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31450.1942728727738151No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31270.19316097715857547No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30540.1886516227189925No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30230.18673669138163532No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29880.18457467212978046No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29220.18049772154056842No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28500.17605013907960987No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28010.17302331212701308No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27790.17166432859727573No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC27420.16937876538817204No Hit
TTTATAGGGTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACA26460.16344865544022727No Hit
GTGATGGAAACACCCTCTTGAATTGGTTTCAACAGAGGCCAGGCCAATCTCCAAGGCGCCTCATTTATAGGGTTT25900.1599894246372595No Hit
GTCCAAGGGTGACGGGCAGGGAGAGGGGAGACTGAGTCAGCACAACATCC25500.1575185454922825No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCCTATAAATGAGGCGCCTTGGAGATTGGCCTGGCCTCTGTTGAAA25260.15603601800529632No Hit
CTCGTAGACAGTGATGGAAACACCCTCTTGAATTGGTTTCAACAGAGGCC24810.15325627896719723No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT24780.15307096303132395No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGCTGACTCAGT24480.15121780367259122No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA24470.1511560316939668No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGCTGACTCAGTCTCCCCTCTCC24470.1511560316939668No Hit
CCCTATAAATGAGGCGCCTTGGAGATTGGCCTGGCCTCTGTTGAAACCAATTCAAGAGGGTGTTTCCATCA23540.1454112376818953No Hit
CATTTATAGGGTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCG23540.1454112376818953No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG23250.143619850301787No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGACAGAGG22870.14127251511405886No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22590.13954289971257497No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA22530.13917226784082845No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22410.13843100409733533No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGCTGA22270.1375661963965934No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22020.13602189693098277No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21740.1342922815294989No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC21560.13318038591425926No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21540.1330568419570104No Hit
GGCCTCTGTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGA21390.13213026227764404No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21090.13027710291891131No Hit
ACAGAGGCCAGTGTGTACCTTGCATGCAGTAATAAATCCCAACATCCTCA20870.12891811938917397No Hit
AGGTACACACTGGCCTCTGTCGATCACCTTCGGCCAAGGGACACGACTGG20760.1282386276243053No Hit
GTAATAAATCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG20470.12644724024419698No Hit
GGTCTAATCAAAGCCTCGTAGACAGTGATGGAAACACCCTCTTGAATTGGTTTCAACAGAGGCCAGGCCAATCTC20470.12644724024419698No Hit
CCCCTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAG20330.12558243254345505No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19840.12255560559085825No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19530.12064067425350108No Hit
GGCCAGTGTGTACCTTGCATGCAGTAATAAATCCCAACATCCTCAGCCTC19010.11742853136503101No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18940.11699612751466003No Hit
GGCCAGGCCAATCTCCAAGGCGCCTCATTTATAGGGTTTCTAACCGGGAC18470.11409284451931209No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18400.11366044066894111No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18350.11335158077581899No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18230.11261031703232591No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG18180.11230145713920377No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18060.11156019339571066No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17980.11106601756671527No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17920.11069538569496873No Hit
GTCTACGAGGCTTTGATTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGGGGAGA17810.11001589393010007No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA17750.10964526205835352No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17610.10878045435761156No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17440.10773033072099636No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17150.10593894334088803No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG17100.10563008344776594No Hit
AGTGTGTACCTTGCATGCAGTAATAAATCCCAACATCCTCAGCCTCCACC16860.10414755596077974No Hit
CTCCAAGGCGCCTCATTTATAGGGTTTCTAACCGGGACTCTGGGGTCCCA16580.10241794055929586No Hit
GTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTC16500.10192376473030045No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16490.10186199275167604No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16380.10118250098680737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)