FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618857
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63690.39342573185895974No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61840.3819979158134412No Hit
GTGTGTACCTTGCATGCAGTAATAAATCCCAACATCCTCAGCCTCCACCC49470.3055859782550281No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40910.2527091645525207No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40730.25159726893728107No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38240.23621604625979936No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37880.23399225502932006No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAATCAAAGCCTCGTA35080.2166961010144812No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32680.20187082614461935No Hit
GTACACACTGGCCTCTGTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT31250.1930374332013266No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30760.1900106062487298No Hit
GGTACACACTGGCCTCTGTCGATCACCTTCGGCCAAGGGACACGACTGGA30440.18803390293274821No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29330.1811772133054371No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27970.17277622421251537No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC27650.17079952089653377No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27620.17061420496066051No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26430.163263339504354No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26360.16283093565398304No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25600.15813626527852676No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25440.15714791362053596No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCCTATAAATGAGGCGCCTTGGAGATTGGCCTGGCCTCTGTTGAAA25370.156715509770165No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24450.15103248773671796No Hit
GTCCAAGGGTGACGGGCAGGGAGAGGGGAGACTGAGTCAGCACAACATCC24380.15060008388634696No Hit
TTTATAGGGTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACA23960.14800566078412114No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23620.1459054135108907No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23560.14553478163914418No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23190.14324921843004046No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGCTGACTCAGT22900.14145783104993215No Hit
CTCGTAGACAGTGATGGAAACACCCTCTTGAATTGGTTTCAACAGAGGCC22780.14071656730643903No Hit
GTGATGGAAACACCCTCTTGAATTGGTTTCAACAGAGGCCAGGCCAATCTCCAAGGCGCCTCATTTATAGGGTTT22740.14046947939194135No Hit
AGGTACACACTGGCCTCTGTCGATCACCTTCGGCCAAGGGACACGACTGG22580.13948112773395058No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGCTGACTCAGTCTCCCCTCTCC22490.13892517992633074No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGACAGAGG22030.1360836689096072No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21760.13441582548674774No Hit
GTAATAAATCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG21720.13416873757225004No Hit
CCCTATAAATGAGGCGCCTTGGAGATTGGCCTGGCCTCTGTTGAAACCAATTCAAGAGGGTGTTTCCATCA21380.13206849029901963No Hit
CATTTATAGGGTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCG21320.13169785842727308No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC21280.13145077051277537No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA21220.13108013864102883No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20920.1292269792822961No Hit
GGCCTCTGTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGA20750.12817685564568088No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG20190.1247176248427131No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19730.12187611382598958No Hit
CCCCTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAG19580.12094953414662321No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19560.12082599018937437No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGCTGA19480.12033181436037896No Hit
ACAGAGGCCAGTGTGTACCTTGCATGCAGTAATAAATCCCAACATCCTCA19150.11829333906577294No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19100.11798447917265084No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19030.11755207532227986No Hit
GGTCTAATCAAAGCCTCGTAGACAGTGATGGAAACACCCTCTTGAATTGGTTTCAACAGAGGCCAGGCCAATCTC18980.11724321542915773No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18890.1166872676215379No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG18710.11557537200629828No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18690.11545182804904944No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA18600.1148958802414296No Hit
GGGTGGAGGCTGAGGATGTTGGGATTTATTACTGCATGCAAGGTACACAC18530.11446347639105864No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18430.11384575660481437No Hit
GGCCAGTGTGTACCTTGCATGCAGTAATAAATCCCAACATCCTCAGCCTC17820.1100776659087245No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17630.10890399831486042No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG17610.10878045435761156No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17520.10822450654999174No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA17480.10797741863549405No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17380.10735969884924981No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17250.1065566631271323No Hit
GTCTACGAGGCTTTGATTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGGGGAGA16920.10451818783252628No Hit
GCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCC16850.1040857839821553No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16630.10272680045241797No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16540.10217085264479814No Hit
AGTGTGTACCTTGCATGCAGTAATAAATCCCAACATCCTCAGCCTCCACC16390.10124427296543177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3500.041.4332851
TGGTATC3700.038.278622
GTATCAA30100.035.069281
GACAGGT3100.033.724771
GATTCAC2950.032.0099531
CGATAGA553.434855E-431.08147237
GCGATAG553.458897E-431.04451836
ATCAACG35550.029.5014213
TCAACGC35750.029.2465674
TATCAAC36150.029.1041582
CAACGCA36300.028.7105225
AATGGTA4550.028.57087170
AACGCAG36600.028.3830386
CGGGATA606.190915E-428.1065794
ATTCACT3450.027.3682922
ACGCAGA38300.027.2993417
CGCAGAG38200.027.281678
CGCAAGT500.00858438327.01738421
AGAGTAC38700.027.00883311
GGTATCA21000.026.4980341
Produced by FastQC (version 0.11.5)