FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891269
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60340.31904504330161393No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC54950.2905456600832563No Hit
GTAATAGACAGCCGTGTCCTCGACTCTCAGACTGTTCATTTGTAGATATA53780.2843593375664699No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGGCGAACACCCAGGTGTTAGTAGTTGTATATGAGCTG48860.25834505826511195No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG42800.22630308010124417No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG41770.22085700130441516No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG41690.220434004893011No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37990.20087042086556697No Hit
GATTATTACTGCAGCTCATATACAACTACTAACACCTGGGTGTTCGCCGGAGGGACCAAGCTGACCGTCCTGGGT36210.19145875071182364No Hit
GTCTATTACTGTGCCAGGGAGACCTTCCAGCACTGGGGCCAGGGCACCCT34270.18120108773527194No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT32740.17311128136716672No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC32410.1713664211701244No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC31760.1679295753274653No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA28860.15259595541406326No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28810.15233158265693564No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA27700.14646250744870243No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT27340.14455902359738357No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG27180.14371303077457517No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAACTACTAACACCTGGGTGTTCG26900.14223254333466048No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAACTATGTCTCCTGGTGCCAACAGCA26480.14001181217478847No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAACTATGTCTCCTGGTGCCAACAGCACCCAGGCAAAGC26000.1374738337063633No Hit
CTCCTGTGCAGTCTCTGGAGTCACCTTCAGTAGCTACTGGATGCACTGGGTCCGCCAAGCTCCAGGGAACGGG25970.13731521005208672No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24890.13160475849813008No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT24400.1290139054782794No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24240.128167912655471No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23590.12473106681281192No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG23450.12399082309285459No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22670.11986660808166369No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22530.11912636436170634No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21810.1153193966590686No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG21520.1137860346677284No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21500.11368028556487733No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC21340.11283429274206895No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20760.10976756875938855No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20110.10633072291672946No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC20060.10606635015960184No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG19700.10416286630828295No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19440.10278812797121933No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC19280.10194213514841094No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19130.10114901687702806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAC352.4934014E-548.5218731
GGTATCA15200.041.5628131
GGATGGG16900.041.3459570
GTGGTAT4200.039.6261941
GTATCAA38750.036.1127931
TGGTATC5000.033.2868842
TATCAAC43450.031.8164482
ATCAACG43850.031.6003343
TCAACGC44100.031.34754
CAACGCA45300.030.5171015
AACGCAG45600.030.3155296
ATACCGC450.004939044430.2554127
ACGTACA450.004989829430.1921842
ACGCAGA47450.029.2051547
CGCAGAG47850.029.1029828
GACAGGT3800.028.6023651
AGAGTAC49050.028.31349811
GGTACGG605.941528E-428.3051764
CAGAGTA50200.027.7383910
GCAGAGT50250.027.6453959
Produced by FastQC (version 0.11.5)