FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004689255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1899588
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58260.3066980840055844No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51420.2706902759966898No Hit
GTGTTAGCAGTTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC48430.2549500207413397No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA42170.22199550639401808No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39760.2093085448002409No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39100.20583410718534753No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCT38690.20367574442458047No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37820.1990958039322211No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31620.16645714754988977No Hit
GTCTGGGACAGACTTCACTCTCTCCATCAGCAGCCTAGATCCTGAAGATT31340.16498314371326836No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT31230.1644040707774528No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30570.16092963316255948No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29880.15729726656517096No Hit
GTTTATTACTGTCAGCATCGTGGCAACTGGCCCACGTGGGCGTTCGGCCA29640.15603383470520976No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29340.15445454488025823No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29310.15429661589776308No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29170.1535596139794524No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29140.15340168499695725No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA27320.14382066005891805No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26710.14060943741484996No Hit
GTAATAAACTGCAAAATCTTCAGGATCTAGGCTGCTGATGGAGAGAGTGAAGTCTGTCCCAGACCCACTGCCA26620.1401356504673645No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26500.13950393453738388No Hit
GTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCTGCAGGGCCAGTCACAGTGTTAGCAGTTACTTAGCCTGGTA26410.13903014758989843No Hit
CTCCATCAGCAGCCTAGATCCTGAAGATTTTGCAGTTTATTACTGTCAGC26090.13734557177661683No Hit
GTCACAGTGTTAGCAGTTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATA25830.1359768539283255No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25750.1355557099750051No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC25310.1332394182317429No Hit
GACTTCACTCTCTCCATCAGCAGCCTAGATCCTGAAGATTTTGCAGTTTATTACTGTCAGCATCGTGGCAACTGG25180.13255505930759723No Hit
CTCCTGCAGGGCCAGTCACAGTGTTAGCAGTTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT24430.12860683474521845No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGCCCACGTGGGCCAGTTGCCACGATG23960.12613261401946105No Hit
GCCTAGATCCTGAAGATTTTGCAGTTTATTACTGTCAGCATCGTGGCAAC22610.11902580980717925No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22490.11839409387719864No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT22130.11649894608725682No Hit
GTAATAGACGGCTGTGTCCTCGGTTTTCAGGCTGTCCATTTGCAGATACA22020.11591987315144126No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21880.11518287123313056No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCACAGTGTTAGCAGTTACTTAGCCTGGTACCAACAGAAACCTGGCCAGG21790.1147090842856451No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21210.11165579062407216No Hit
CTGCTAACACTGTGACTGGCCCTGCAGGAGAGGGTGGCTCTGTCCCCTGGAGACAAAGACAGGGTGGCTGGAGA21020.1106555737349362No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20810.10955007085747014No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20780.10939214187497501No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20640.10865513995666429No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20620.10854985396833419No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGTTTCATTTTCAGTACCGCCTGG20360.10718113612004287No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20290.10681263516088753No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19920.10486484437678065No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19180.10096926280856691No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19030.10017961789609116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)