FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1899588
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59610.3138048882178662No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55460.2919580456393702No Hit
GTGTTAGCAGTTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC52740.2776391512264765No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43790.23052367144875624No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA42120.22173229142319284No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39670.20883475785275543No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCT39410.20746604000446414No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38220.2012015236988231No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35550.18714584425675462No Hit
GTCTGGGACAGACTTCACTCTCTCCATCAGCAGCCTAGATCCTGAAGATT31610.1664045045557247No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30920.16277213795833623No Hit
GTTTATTACTGTCAGCATCGTGGCAACTGGCCCACGTGGGCGTTCGGCCA29270.1540860439211029No Hit
GTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCTGCAGGGCCAGTCACAGTGTTAGCAGTTACTTAGCCTGGTA28990.15261204008448148No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28570.15040103432954935No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28550.15029574834121925No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT28490.14997989037622894No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28060.14771624162713176No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27960.14718981168548126No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA27540.14497880593054915No Hit
GACTTCACTCTCTCCATCAGCAGCCTAGATCCTGAAGATTTTGCAGTTTATTACTGTCAGCATCGTGGCAACTGG27220.14329423011726752No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26920.14171494029231602No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26830.14124115334483056No Hit
GTAATAAACTGCAAAATCTTCAGGATCTAGGCTGCTGATGGAGAGAGTGAAGTCTGTCCCAGACCCACTGCCA26430.13913543357822855No Hit
CTCCTGCAGGGCCAGTCACAGTGTTAGCAGTTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT26280.13834578866575278No Hit
GTCACAGTGTTAGCAGTTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATA25730.135450423986675No Hit
CTCCATCAGCAGCCTAGATCCTGAAGATTTTGCAGTTTATTACTGTCAGC25580.13466077907419924No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24610.12955440864018936No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24230.1275539748619174No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23830.1254482550953154No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23790.1252376831186552No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22910.12060509963213076No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC22610.11902580980717925No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCACAGTGTTAGCAGTTACTTAGCCTGGTACCAACAGAAACCTGGCCAGG22270.11723594800556753No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGCCCACGTGGGCCAGTTGCCACGATG22210.11692009004057721No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT22200.11686744704641217No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22130.11649894608725682No Hit
CTGCTAACACTGTGACTGGCCCTGCAGGAGAGGGTGGCTCTGTCCCCTGGAGACAAAGACAGGGTGGCTGGAGA21820.11486701326814024No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21720.11434058332648975No Hit
GCCTAGATCCTGAAGATTTTGCAGTTTATTACTGTCAGCATCGTGGCAAC21390.11260336451904308No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGTTTCATTTTCAGTACCGCCTGG20670.10881306893915944No Hit
CTGCTACTCTGGCTCCCAGATATCACCGGAGAAATTGTTTTGACACAGTC20570.10828663899750894No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20110.10586506126591662No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19710.10375934149931458No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19330.10175890772104265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAA901.2779692E-648.578970
CCGTTTA602.1129763E-645.16190369
GGTATCA17200.044.5795361
GTGGTAT4250.042.1235581
TACGTAT959.9854355E-538.35176570
TGGTATC4900.037.2211722
GTATCAA36850.031.3492181
GACAGGT3400.030.7978861
CTAATGG1203.9389433E-430.36181470
AATGGTA8250.029.14734370
TATCAAC42200.027.0518282
ATCAACG42400.026.848813
ACCGTTT758.6028094E-426.5678668
TCAACGC42900.026.4585424
CAACGCA43550.026.1391415
AACGCAG43950.026.0549586
CGCAGAG45450.025.1950598
GTACGTA900.001201282125.08994569
ACGCAGA45800.025.002527
AGAGTAC46750.024.48928611
Produced by FastQC (version 0.11.5)