FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359333
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAAACATCTCCTGCACCGGCAGCAGTGGCAACATTGCCAG46570.3425944930344515No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAAACATCTCCTGCACCGGCAGCAGTGGCAACATTGCCAGCAACTATGTG40560.29838163275665347No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37520.2760177233981666No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCTTGATCGGTACTATCATAAGACTGACAGTAGTAGT36930.2716773594108287No Hit
ATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAG36640.2695439601628151No Hit
TCTCTGGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT36420.2679255193539773No Hit
GTACTATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAG34500.2538009450223014No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC34100.25085832536986885No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32840.2415890734647066No Hit
GTCTTATGATAGTACCGATCAAGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGC32660.24026489462111197No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCTTGATCGGTACTATCATAAGACTGA32110.2362187925990173No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31470.23151060115512534No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC31350.23062781525939557No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA28720.21128009104465204No Hit
TGATAGTACCGATCAAGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC28190.20738112000517903No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAAA27040.19892108850443563No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26960.19833256457394913No Hit
GACTACTACTGTCAGTCTTATGATAGTACCGATCAAGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGT26530.1951692484475842No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26100.19200593232121932No Hit
GTAATATATGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCCTCAGGGAGA26030.1914909738820436No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGAGG24870.18295737688998942No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24550.18060328116804344No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACG24490.18016188822017856No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23860.1755272622675974No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGA23850.1754536967762866No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23580.17346742851089467No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23130.17015698140190816No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22940.16875923706700272No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22380.16463956955359724No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21820.16051990204019176No Hit
CTACTACTGTCAGTCTTATGATAGTACCGATCAAGGGGTGTTCGGCGGAG21790.16029920556625935No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21760.16007850909232688No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20900.15375187683959707No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20900.15375187683959707No Hit
CCCCTTGATCGGTACTATCATAAGACTGACAGTAGTAGTCAGCCTCGTCC20840.15331048389173219No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20750.1526483944699349No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT20480.15066212620454297No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTT20360.1497793403088132No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20180.14845516146521862No Hit
ATCAAGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCC20040.14742524458686723No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19940.1466895896737591No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC19130.14073078487758334No Hit
GAGTTGGAGGAGCTGTCGATGGAGCCAGAGAAACGATCGGGGACCCCAGA18930.1392594750513671No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18430.1355812004858265No Hit
GCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCT18360.13506624204665083No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18280.13447771811616432No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC18160.1335949322204346No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGA17830.1311672710071778No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17670.12999022314620481No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC17430.12822465135474528No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC16700.12285437048905604No Hit
CCCCAGAGGGTCTTTGGTCATTCTCATAGATCACAGTGGTGGGGGCACTGCCCGGGCGCTGCTGGTACCACTGCA16200.11917609592351543No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15870.11674843471025864No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTC15810.11630704176239376No Hit
GAGTGGGGCTGAGTCAGCATAAAATTGGCCCAAGAACCTGTGCAGTGAGCGAGGAGGGTGAGAAGTAGTGGAGCC15660.11520355939273158No Hit
GTCTCTGGTGGCTCCATCAGTACTTACTACTGGAGCTGGATCCGGCAGCC15150.11145171933588016No Hit
GGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAAACATCTC15090.1110103263880153No Hit
GCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCG14760.1085826651747585No Hit
GTCTTCAGTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCAGAGAAACGATCGGGGACC14420.1060814384701909No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14290.10512508708315034No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14160.10416873569610978No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14140.10402160471348816No Hit
CCTCCAACTCTGCCTCCCTCACCATCTCTGGACTGAAGACTGAGGACGAG14000.10299168783513679No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC14000.10299168783513679No Hit
CTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACT13960.10269742586989355No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG13960.10269742586989355No Hit
GGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCC13860.10196177095678541No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13730.10100541956974488No Hit
CTCCTGCACCGGCAGCAGTGGCAACATTGCCAGCAACTATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCC13660.10049046113056918No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)