FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359333
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75430.5549045009574548No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAAACATCTCCTGCACCGGCAGCAGTGGCAACATTGCCAG42410.3119912486491537No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG40950.3012506869177751No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAAACATCTCCTGCACCGGCAGCAGTGGCAACATTGCCAGCAACTATGTG39610.29139291108212634No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCTTGATCGGTACTATCATAAGACTGACAGTAGTAGT37250.27403145513277466No Hit
ATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAG36560.26895543623232865No Hit
TCTCTGGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT35000.257479219587842No Hit
GTACTATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAG34910.2568171301660447No Hit
GTCTTATGATAGTACCGATCAAGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGC34770.2557872132876933No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC32570.23960280519931465No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32060.23585096514246326No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCTTGATCGGTACTATCATAAGACTGA30880.22717023716778742No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA29170.2145905381536386No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29000.21333992480135477No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC28360.20863173335746282No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA28040.20627763763551685No Hit
GTAATATATGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCCTCAGGGAGA26700.19641986179986803No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT25990.19119671191680038No Hit
GACTACTACTGTCAGTCTTATGATAGTACCGATCAAGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGT25750.18943114012534087No Hit
TGATAGTACCGATCAAGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC25490.18751843735125978No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAAA25080.18450225220751648No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG24600.1809711086245975No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGAGG24440.17979406076362453No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23840.1753801312849758No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23580.17346742851089467No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACG23370.17192255319336763No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGA23040.16949489198011083No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22370.16456600406228644No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21980.16169694990116476No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC21490.158092240826935No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21360.15713588943989443No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG20730.15250126348731327No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20230.14882298892177265No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20150.14823446499128617No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19980.14698385163900235No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19830.14588036926934017No Hit
CCCCTTGATCGGTACTATCATAAGACTGACAGTAGTAGTCAGCCTCGTCC19440.14301131510821852No Hit
GAGTTGGAGGAGCTGTCGATGGAGCCAGAGAAACGATCGGGGACCCCAGA19430.1429377496169077No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG19240.14154000528200228No Hit
CTACTACTGTCAGTCTTATGATAGTACCGATCAAGGGGTGTTCGGCGGAG19110.14058365389496172No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18730.13778816522515086No Hit
ATCAAGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCC18690.13749390325990762No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18370.13513980753796165No Hit
CCCCAGAGGGTCTTTGGTCATTCTCATAGATCACAGTGGTGGGGGCACTGCCCGGGCGCTGCTGGTACCACTGCA17780.13079944355062373No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17590.1294016992157183No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC17520.1288867407765426No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC17120.12594412112411013No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16850.12395785285871821No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16510.1214566261541506No Hit
GAGTGGGGCTGAGTCAGCATAAAATTGGCCCAAGAACCTGTGCAGTGAGCGAGGAGGGTGAGAAGTAGTGGAGCC15620.11490929742748834No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTC15600.1147621664448667No Hit
GTCTTTGGTCATTCTCATAGATCACAGTGGTGGGGGCACTGCCCGGGCGCTGCTGGTACCACTGCACATAGTTG15560.11446790447962346No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15550.11439433898831265No Hit
GTCTTCAGTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCAGAGAAACGATCGGGGACC15520.11417364251438021No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15310.11262876719685318No Hit
GTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTATGAGAATGACCAAAGACCCTCTGGGGTCCC15260.1122609397402991No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14990.11027467147490717No Hit
GGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAAACATCTC14900.10961258205310986No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14430.10615500396150171No Hit
GTCTCTGGTGGCTCCATCAGTACTTACTACTGGAGCTGGATCCGGCAGCC14340.1054929145397044No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG14230.10468369413528546No Hit
GCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCT14130.10394803922217735No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC13820.10166750899154217No Hit
GTGGTGGGGGCACTGCCCGGGCGCTGCTGGTACCACTGCACATAGTTGCTGGCAATGTTGCCACTGCTGCCGGTG13650.10041689563925837No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGA13600.10004906818270431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12550.042.4319271
GTATCAA31050.037.7743531
TAACAGG850.002280311735.4224270
TGATAGC400.002909804533.70755813
GCTTATA601.5754656E-533.703841
TATCAAC35350.032.705142
AATGGTA3850.031.28213770
ACGGGTC1307.2759576E-1231.12612716
ATCAACG37300.030.9049913
CAACGCA37500.030.650285
ACAGGCC3300.030.6398541
AACGCAG37550.030.6094676
TCAACGC37600.030.4779974
ACGCAGA39200.029.3210587
AGAGTAC39400.029.25238611
CGCAGAG39200.029.2350738
CAGAGTA40100.028.57892610
GAGTTTA4500.028.4610211
GAGCGAT705.9348106E-428.309568
GTGGTAT3850.027.1381571
Produced by FastQC (version 0.11.5)