FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257940
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG102980.8186399987280792No Hit
GAGTTTACTCTCACCATCAGCAGCCTACAGTCTGAAGATTTTGCGGTTTATTACTGTCAGCAGGTTCATAACTGG54790.4355533650253589No Hit
GTTATGAACCTGCTGACAGTAATAAACCGCAAAATCTTCAGACTGTAGGCTGCTGATGGTGAGAGTAAACT51600.41019444488608364No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48780.38777684150277436No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA45320.3602715550821184No Hit
GTTCATAACTGGCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT43320.34437254559040975No Hit
GAGTAAACTCTGTCCCAGACCCACTGCCACTGAATCTGGCTGGGATACCA40680.32338585306135426No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35610.28308186399987284No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35200.27982256705407255No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33140.2634465872776126No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGATTCAGTGGCAGTGGGTCTGGGACAGAGTTTA33020.26249264670811007No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31990.2543046568198801No Hit
GTCTGGGACAGAGTTTACTCTCACCATCAGCAGCCTACAGTCTGAAGATT27580.2192473408906625No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCGACTTAGGCTGGTA26890.21376218261602303No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGCC26810.2131262222363547No Hit
CTGGTATCCCAGCCAGATTCAGTGGCAGTGGGTCTGGGACAGAGTTTACT26790.2129672321414376No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC25910.2059716679650858No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25800.20509722244304182No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCGACTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT25660.2039842917786222No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25460.20239439082945132No Hit
GTTTATTACTGTCAGCAGGTTCATAACTGGCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA24310.19325246037171884No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23940.19031114361575271No Hit
GTCCCAGACCCACTGCCACTGAATCTGGCTGGGATACCAGTGGCCCTGGT23400.18601841105299138No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23030.1830770942970253No Hit
GTAATAAACCGCAAAATCTTCAGACTGTAGGCTGCTGATGGTGAGAGTAAACTCTGTCCCAGACCCACTGCCA22650.18005628249360064No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22530.17910234192409813No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22250.1768764805952589No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22220.17663799545288328No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT21620.17186829260537068No Hit
GTGTTAGCAGCGACTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCA21430.17035788670365837No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21260.16900647089686313No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG20970.16670111452056535No Hit
GTCAGAGTGTTAGCAGCGACTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG19960.15867211472725248No Hit
GGAGAAATAGTGATGACGCAGTCGCCAGCCACCCTGTCTGTGTCTCCAGG19790.15732069892045725No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19050.15143806540852506No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18970.1508021050288567No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18770.14921220407968583No Hit
CTGCTACTCTGGCTCCCAGATATCACTGGAGAAATAGTGATGACGCAGTC18710.14873523379493458No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18640.14817876846272476No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18580.14770179817797352No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18540.14738381798813932No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGC18180.14452199627963178No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15940.12671510564891808No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15910.12647662050654243No Hit
CAGTAATAAACCGCAAAATCTTCAGACTGTAGGCTGCTGATGGTGAGAGTAAACTCTGTCCCAGACCCACTGCCA15740.12512520469974722No Hit
GTCTGAAGATTTTGCGGTTTATTACTGTCAGCAGGTTCATAACTGGCCGC15680.12464823441499595No Hit
GAGTGTTAGCAGCGACTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATC15560.12369429384549342No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15540.12353530375057635No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15450.12281984832344946No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCACTGGAGAAATAGTGATGACGCAGTCGCCAGCCACC15360.12210439289632255No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15320.12178641270648839No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15060.11971954147256625No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14760.11733469004880998No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGATT14640.11638074947930743No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14620.11622175938439036No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCGACTTAGG14520.11542680890980492No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14140.11240599710638027No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14100.1120880169165461No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14090.11200852186908755No Hit
GGTTTCTGCTGGTACCAGCCTAAGTCGCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC14030.1115315515843363No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13990.11121357139450211No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGCCAGTT13980.11113407634704357No Hit
TTATTACTGTCAGCAGGTTCATAACTGGCCGCTCACTTTCGGCGGAGGGA13920.11065710606229232No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13730.10914670016057999No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13700.10890821501820436No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCA13620.10827225463853603No Hit
TCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGATTCAGTGGCAGTGGGTCTGGGACAGAGTTTACT13450.10692083883174078No Hit
CCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGATTCA13420.10668235368936514No Hit
CACCAGGGCCACTGGTATCCCAGCCAGATTCAGTGGCAGTGGGTCTGGGACAGAGTTTACTCTCACCATCAGCAG13330.10596689826223828No Hit
GCCTACAGTCTGAAGATTTTGCGGTTTATTACTGTCAGCAGGTTCATAACTGGCCGCTCACTTTCGGCGGAGGGA13280.10556942302494554No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCACTGGAGAAATAGTGATGACGCAGTCGC13220.10509245274019428No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGCCTAAGTCGCTGCTA13090.10405901712323322No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13040.10366154188594051No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12880.10238962112660381No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12830.1019921458893111No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12830.1019921458893111No Hit
GGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCGACTTAGGCTGGTACCAGCAGAAAC12790.10167416569947692No Hit
GACTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCA12680.10079972017743295No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)