FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1808530
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC93900.5192062061453224No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTGGGTGCCTCCTCCGAACACAACATGATCACCATTACTATCCCACAC82290.4550104228295909No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG70000.38705467976754604No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCGTAAGT46790.25871840666176393No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTGGGTGCCTC45690.2526361188368454No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGATACCAGTGCACACTTTTATCT44020.24340210004810536No Hit
CCCCAGGACAGACGGCCAGGTTTACCTGTGGGGGAAACAACATTGGAGAT43420.24008448850724068No Hit
GGTTTACCTGTGGGGGAAACAACATTGGAGATAAAAGTGTGCACTGGTATCAGCAGAAGCCAGGCCAGGCCC42350.23416808125936533No Hit
GCCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGTTTAC40380.223275256700193No Hit
CTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG38740.21420711848849616No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCT36670.20276135867251305No Hit
CTTTTATCTCCAATGTTGTTTCCCCCACAGGTAAACCTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGG35540.1965131902705512No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG35280.1950755586028432No Hit
CCCCTGTGGTGGTCGTCTATGATGACACCGGCCGGCCCTCAGGGATCCCT34950.19325087225536763No Hit
TTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGGTTCGAC34160.18888268372656247No Hit
CCATTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGGTTC33420.18479096282616267No Hit
GTTTGGTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAG32720.1809204160284872No Hit
GTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG32690.180754535451444No Hit
CTATTACTGTCAGGTGTGGGATAGTAATGGTGATCATGTTGTGTTCGGAG31990.17688398865376853No Hit
GGCTCAGGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGA30990.17135463608566073No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTGACCTCCTATGTGCTGACT30980.17129934255997967No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTGA30350.16781585044207173No Hit
GACCATCACCAGCGTCGAACCCGGGGATGAGGCCGACTATTACTGTCAGG30100.16643351230004477No Hit
CCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG29970.16571469646619078No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT29560.16344766191326657No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC27810.15377129491907793No Hit
GGGCTGGATGACTTGGGATGGGGGCAGAGACCCCTCCCCTGGGAACCTGC27440.15172543446887804No Hit
ACACTGGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC27230.1505642704295754No Hit
GGCCAGGCCCCTGTGGTGGTCGTCTATGATGACACCGGCCGGCCCTCAGG27120.14995604164708354No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC26920.148850171133462No Hit
GGATAGTAATGGTGATCATGTTGTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTCAGCCCAAGGCTGCCCC26620.14719136536302965No Hit
GTAATACACGCCCGTGTCCGCAGCGGTAACAGACGTCAGCCTCAGGGAGAACTGATTCTTGGACGTGTCCACTGA26550.14680431068326208No Hit
GGGTGGCCGTGTTGCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGCCGGTGTCATCATAGA26400.14597490779804592No Hit
CCTCTCTCACTGCACAGCCTCTGTGACCTCCTATGTGCTGACTCAGCCAC26190.14481374375874329No Hit
GGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA25770.142491415680138No Hit
GGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGACGGGG25750.14238082862877585No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC25430.14061143580698135No Hit
GCTGATACCAGTGCACACTTTTATCTCCAATGTTGTTTCCCCCACAGGTAAACCTGGCCGTCTGTCCTGGGGCCA25370.1402796746528949No Hit
GGTGATGGTCAGGGTGGCCGTGTTGCCAGAGTTGGAGCCAGAGAATCGCT25160.13911851061359226No Hit
CGGCCACCCTGACCATCACCAGCGTCGAACCCGGGGATGAGGCCGACTAT25150.13906321708791117No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGGTTCGACGCTGG25020.13834440125405717No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTGACCTCCTA24700.13657500843226267No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG24370.1347503220847871No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCATACTT24360.134695028559106No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCGTAAGTGACTTCTACC23860.13193035227505212No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTGACCTCCTATGTGCTGACTCAGC23470.12977390477349007No Hit
CTGCACAGCCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGT23370.1292209695166793No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT23360.1291656759909982No Hit
GGTATCAGCAGAAGCCAGGCCAGGCCCCTGTGGTGGTCGTCTATGATGACACCGGCCGGCCCTCAGGGATCC23350.12911038246531714No Hit
CCCCTCCCCTGGGAACCTGCAGCTCCAGGCCCCTGTGGGTGGGGTGAGGG23310.12888920836259282No Hit
GTAATAGTCGGCCTCATCCCCGGGTTCGACGCTGGTGATGGTCAGGGTGG22770.12590335797581462No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTACGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG22740.1257374773987714No Hit
GTGTCATCATAGACGACCACCACAGGGGCCTGGCCTGGCTTCTGCTGATA22660.12529512919332275No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGG22340.12352573637152825No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGTTTACCTGTGGGGGAAACAACATTGGAGATAAAAGTGTG22330.12347044284584717No Hit
CTCACTGCACAGCCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG22260.12308338816607964No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACGGCTC22240.12297280111471748No Hit
CAGTAATAGTCGGCCTCATCCCCGGGTTCGACGCTGGTGATGGTCAGGGT21820.1206504730361122No Hit
GGATATTTATTGAGAAGGGCTGGATGACTTGGGATGGGGGCAGAGACCCCTCCCCTGGGAACCTGCAGCTCCAGG21760.12031871188202574No Hit
GCCATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21690.11993165720225818No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGCAACACGG21230.1173881550209286No Hit
GGCCTGGCCTGGCTTCTGCTGATACCAGTGCACACTTTTATCTCCAATGTTGTTTCCCCCACAGGTAAACCTGGC21150.11694580681547997No Hit
CCACCAAACCCTCCAAACAAAGCAACAACAAGTATGCGGCCAGCAGCTAC20670.11429171758278822No Hit
GACTATTACTGTCAGGTGTGGGATAGTAATGGTGATCATGTTGTGTTCGG20610.11395995642870176No Hit
CAGTAATACACGCCCGTGTCCGCAGCGGTAACAGACGTCAGCCTCAGGGAGAACTGATTCTTGGACGTGTCCA20530.11351760822325314No Hit
GGGATCCCTGAGGGCCGGCCGGTGTCATCATAGACGACCACCACAGGGGC20370.11263291181235588No Hit
GACCGAGGACGGTCAGCTGGGTGCCTCCTCCGAACACAACATGATCACCA20350.11252232476099373No Hit
CAGGTAAACCTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGGCTG20320.1123564441839505No Hit
AGATAAAAGTGTGCACTGGTATCAGCAGAAGCCAGGCCAGGCCCCTGTGG20100.11113998661896679No Hit
GTCCTGGGGCCACTGACACCGAGGGTGGCTGAGTCAGCACATAGGAGGTC19980.11047646431079385No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTGGGTGCCTCCTCCGAACACAACATGATCAC19720.10903883264308581No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG19460.1076012009753778No Hit
GGGTAGAAGTCACTTACGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19410.10732473334697241No Hit
TAGTAATGGTGATCATGTTGTGTTCGGAGGAGGCACCCAGCTGACCGTCC19370.10710355924424808No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTG19320.1068270916158427No Hit
GTAATGGTGATCATGTTGTGTTCGGAGGAGGCACCCAGCTGACCGTCCTC19300.10671650456448055No Hit
GGTATCAGCAGAAGCCAGGCCAGGCCCCTGTGGTGGTCGTCTATGATGAC19300.10671650456448055No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTACGAGACACACCAGTGTGGCC19160.10594239520494546No Hit
GTGTCAGTGGCCCCAGGACAGACGGCCAGGTTTACCTGTGGGGGAAACAA19140.1058318081535833No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19130.1057765146279022No Hit
AGCCAGGCCAGGCCCCTGTGGTGGTCGTCTATGATGACACCGGCCGGCCC19000.10505769879404822No Hit
CTCCAGGCCCCTGTGGGTGGGGTGAGGGTCGGGGGCCTAAGAGCATTCTG18440.10196126135590784No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCA18300.10118715199637274No Hit
CAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGCAACACGGCCACC18130.10024716205979442No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)