FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319604
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCACCAATAGGAACTACTTAGCTTGGTACCAGCAGAA87380.6621683474739392No Hit
CTATTATGCTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT80770.6120775626627382No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52370.39686148268723037No Hit
GCATAATAGTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC44980.3408598337076881No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43520.32979590846951057No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAAGGAGTCTGGAGA40850.3095625657394188No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35150.26636778912461617No Hit
ATGCTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA34850.2640943798291002No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33430.25333357583032484No Hit
GAGTAGCATAATAGTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT31830.24120872625423992No Hit
CTCCTTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAC30890.23408537712829No Hit
GTTTATTACTGTCAACACTATTATGCTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA29290.22196052755220505No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28000.21218486758148658No Hit
TCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCTGTATAAAACACT26340.19960533614629844No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26340.19960533614629844No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26270.1990748739773447No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26210.19862019211824156No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26160.19824129056898887No Hit
ACCCGGGACTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC25160.1906632595839358No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG24950.18907187307707465No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24860.18838985028841987No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24840.1882382896687188No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24490.18558597882395023No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22720.17217286398040624No Hit
AACCAGGACAGCCTCCTAAGATGCTCATTTACTGGGCATCTACCCGGGAC22510.17058147747354507No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC22220.1683838484878797No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22140.16777760600907546No Hit
CTCCTAAGATGCTCATTTACTGGGCATCTACCCGGGACTCCGGGGTCCCT21970.16648934074161642No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACACTATTATGCTACT20920.15853240820731068No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA20600.1561074382920937No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC20500.15534963519358838No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19890.150727036292706No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19590.14845362699719006No Hit
GTAATACACGGCTGTGTCCTCGGTTTTCAGGCTATTCATTTGCAGATACA19570.14830206637748902No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18360.13913264888557475No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC17540.13291866347783124No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17480.13246398161872805No Hit
GTCTCCAGACTCCTTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA17430.1320850800694754No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16810.12738670085874249No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCGCTTTCAGTAACGCCTGG16780.12715935992919086No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGATGCTCATTTACTGGGCATCTACCCGGGACTC16730.12678045837993823No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16410.12435548846472122No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC16320.12367346567606645No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT16150.12238520040860743No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCTTGGCTGTGTCTCTGGGCGAGAGGG16070.12177895792980317No Hit
GTCAGGGACCCCGGAGTCCCGGGTAGATGCCCAGTAAATGAGCATCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA15650.11859618491608089No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC15550.11783838181757557No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15330.1161712150008639No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15260.11564075283191017No Hit
CCTTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC15160.11488294973340486No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCTG15010.1137462450856469No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT14620.11079081300147621No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCACCAATAGGAACTA14570.11041191145222354No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14550.11026035083252247No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14460.10957832804386769No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14450.10950254773401719No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14380.10897208556506345No Hit
CTCCTGTGCAGCCTCTGGATTCGCTTTCAGTAACGCCTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG14370.10889630525521292No Hit
GTCCCGGGTAGATGCCCAGTAAATGAGCATCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTT14260.1080627218468571No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14110.10692601719909911No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14060.10654711564984647No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14050.10647133533999593No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGATGCTCATTTACTGGGCATCTACCCGGGA13960.10578931255134116No Hit
GTAAATGAGCATCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA13870.10510728976268638No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13800.10457682759373267No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGATGCTCATT13550.1026823198474694No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC13550.1026823198474694No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13500.10230341829821674No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13430.10177295612926301No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13280.10063625148150507No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATT352.2971799E-549.19777740
TTGTGCG506.023765E-849.1820352
GCGTGTA305.537115E-447.23837756
CGCGTAT1100.046.90239
ATGCCGT3650.046.51861244
TCGCGGA451.917304E-645.8034538
ATCGCGT1650.045.7578237
TGCGTGA1250.045.2432555
TGCATCG2950.045.21757534
CGAGACT8650.045.15668523
ACGAGAC8650.045.14812522
CTTATAC8900.045.014371
CACGAGA8600.045.0106221
GCGTATG1000.044.76997440
GCATCGC2150.044.58094835
CGTATGC3100.044.4982941
TATACAC9150.043.7678833
CATCGCG2200.043.6327836
GTATGCC3800.043.608542
CCGTCTT3200.043.5165547
Produced by FastQC (version 0.11.5)