FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477337
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATTGTCAGCAATATGGCTACTCACCGATCACTTTCGGCGGAGGGA53520.3622734690866065No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52240.35360923066301053No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44290.2997961873289574No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA37390.2530905270767604No Hit
CAATAATATACTGCAAAATCGTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGGCA34880.23610049704299022No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34140.23109148420434877No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33890.2293992501372402No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33470.22655629690449774No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28900.19562225815775275No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCACCCCAGACAGGTTCAGTGCCAGTGGGTCTGGGACAGACTTCA28780.19480998580554065No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28300.19156089639669216No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGACGATT28180.19074862404448004No Hit
GTATATTATTGTCAGCAATATGGCTACTCACCGATCACTTTCGGCGGAGGGACCAAGGTGGATTTCAAACGAA28000.18953021551616187No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGGCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGAGA27730.18770260272368458No Hit
GTCCCAGACCCACTGGCACTGAACCTGTCTGGGGTGCCAGTGGCCCTGCT27060.18316741542383355No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGAGACAACTACCTAGCCTGGTACC26230.17754919832103305No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25800.17463855572560627No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAGGTAGTTGTCTCTAACACTCTGA25750.17430010891218456No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25720.17409704082413152No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25500.17260787484507598No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24650.16685427901690675No Hit
GTGTTAGAGACAACTACCTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT24480.1657035598512729No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24330.16468821941100778No Hit
GTCTTTGTCTCCAGGGGAAAGAGGCACCCTCTCCTGCAGGGCCAGTCAGA24290.1644174619602704No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24240.1640790151468487No Hit
ATAATATACTGCAAAATCGTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGGCA23810.16116837255142194No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAAATCCACCTTGGTCC23800.16110068318873758No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22990.15561784481130575No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22540.1525718234905103No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGGCACCCTCTCCTGC22420.15175955113829817No Hit
GTGCAGCCACAGTTCGTTTGAAATCCACCTTGGTCCCTCCGCCGAAAGTG20130.1362586870835835No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGACGATTTTGCAGTATATTATTGTCAGCAATATGGCTACTCA20020.13551410409405573No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA20010.1354464147313714No Hit
GAGTGAAGTCTGTCCCAGACCCACTGGCACTGAACCTGTCTGGGGTGCCA19880.13456645301647494No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19640.13294190831205066No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18610.1259699039555633No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18570.12569914650482591No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18270.1236684656242956No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17880.1210285804796062No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17810.1205547549408158No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACAC17750.12014861876470974No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17610.11920096768712894No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17490.11838869533491682No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17090.11568112082754307No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16690.11297354632016933No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCACCGGAGAAGTTGTGTTGACGCAGTCTC16450.11134900161574508No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15580.1054600270622072No Hit
CTGCTACTCTGGCTCCCAGACACCACCGGAGAAGTTGTGTTGACGCAGTC15300.10356472490704559No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15110.10227862701604307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)