FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477337
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55030.3724945628519424No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54580.3694485415311469No Hit
ATATTATTGTCAGCAATATGGCTACTCACCGATCACTTTCGGCGGAGGGA49070.33215170269207367No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37590.2544443143304473No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA37540.2541058675170256No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36360.24611852272027304No Hit
CAATAATATACTGCAAAATCGTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGGCA35240.23853731409962656No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32760.22175035215390937No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32070.21707978612868967No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGACGATT29130.1971791134994927No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28710.19433616026675024No Hit
GTGTTAGAGACAACTACCTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT26730.18093366645525022No Hit
GTATATTATTGTCAGCAATATGGCTACTCACCGATCACTTTCGGCGGAGGGACCAAGGTGGATTTCAAACGAA26430.17890298557471992No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGGCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGAGA26020.17612772170466184No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25850.174977002539028No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCACCCCAGACAGGTTCAGTGCCAGTGGGTCTGGGACAGACTTCA25750.17430010891218456No Hit
GTCCCAGACCCACTGGCACTGAACCTGTCTGGGGTGCCAGTGGCCCTGCT25120.17003567906307093No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24930.1687495811720684No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24740.16746348328106586No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGAGACAACTACCTAGCCTGGTACC24460.16556818112590424No Hit
ATAATATACTGCAAAATCGTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGGCA24420.1652974236751669No Hit
GTCTTTGTCTCCAGGGGAAAGAGGCACCCTCTCCTGCAGGGCCAGTCAGA23890.16170988745289666No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23440.1586638661321012No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAGGTAGTTGTCTCTAACACTCTGA23060.15609167035009616No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22860.15473788309640926No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21770.14735974256381582No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGGCACCCTCTCCTGC21760.1472920532011315No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21570.14600595531012897No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21240.14377220634154564No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA20920.14160614673564664No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAAATCCACCTTGGTCC20880.14133538928490927No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20830.14099694247148756No Hit
GTGCAGCCACAGTTCGTTTGAAATCCACCTTGGTCCCTCCGCCGAAAGTG20760.14052311693269715No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20600.13944008712974765No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20510.13883088286558856No Hit
GAGTGAAGTCTGTCCCAGACCCACTGGCACTGAACCTGTCTGGGGTGCCA19890.13463414237915924No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGACGATTTTGCAGTATATTATTGTCAGCAATATGGCTACTCA19600.1326711508613133No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19490.13192656787178553No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17890.12109626984229055No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17750.12014861876470974No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17270.11689952935586125No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17180.11629032509170216No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACAC17100.11574881019022741No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16990.11500422720069964No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16330.11053672926353297No Hit
CTGCTACTCTGGCTCCCAGACACCACCGGAGAAGTTGTGTTGACGCAGTC16190.10958907818595215No Hit
GGGGAAAGAGGCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGAGACAACTACCTAGCCTGGTACCAGCAGAAA16080.10884449519642438No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15960.10803222284421225No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCACCGGAGAAGTTGTGTTGACGCAGTCTC15940.10789684411884357No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15620.10573078451294457No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15600.10559540578757588No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15310.10363241426972992No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15190.10282014191751782No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)