FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048539
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGCCAGCAATATACTACCTCAGGGAGAAATTTTGGCCAGGGGA35640.33990152011513164No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34540.3294107324572572No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29280.27924569329323945No Hit
GTGTATTACTGCCAGCAATATACTACCTCAGGGAGAAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA26600.25368631972678174No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25380.2420510825062301No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22770.21715930451800075No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21500.2050472133130003No Hit
CAGTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT18760.17891561496520397No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18490.17634060344918023No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17900.17071372643268395No Hit
GTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGA17420.16613592818197512No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17350.16546833260374674No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17310.16508684941618765No Hit
CAACTACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCATCCCGCAGGGC17110.1631794334783923No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17060.16270257949394348No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16790.16012756797791974No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT16640.15869700602457326No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16370.15612199450854952No Hit
GTCCCAGACCCACTGCCAGTAAACCTGTCTGCGATGCCAGTGGCCCTGCG15930.15192567944539975No Hit
GTCTCCAGGCTCCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCGTCTCCTGCAGGGCCAGTCAGATTGTTACTCA15810.15078122988272255No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15810.15078122988272255No Hit
GCAATATACTACCTCAGGGAGAAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT15380.1466802856164625No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15300.14591731924134438No Hit
GGTTTACTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGA15230.145249723663116No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15220.14515435286622624No Hit
GTCTGGGGCTGAGGTGAAGAAGCCCGGGTCCTCGGTGAAGATCTCCTGCAAGGCTTCTGAAGTCAGCTTCAACAG14710.1402904422248481No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCATCCCGCA14530.13857376788083228No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14460.1379061723026039No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCAGTAAACCTGTCTGCGATGCCA14110.13456819441146206No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCGTCTCCTGCAGGGCCAGTCAGA14080.13428208202079273No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTCTGCAGTGTA13370.12751075544161924No Hit
TCTCTGGTGCATCCCGCAGGGCCACTGGCATCGCAGACAGGTTTACTGGCAGTGGGTCTGGGACAGACTTCACT13120.12512648551937505No Hit
ATATACTACCTCAGGGAGAAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT13080.12474500233181599No Hit
CTCCAGGCTCCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCGTCTCCTGC13070.12464963153492623No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12990.12388666515980808No Hit
CAATAATAGACGGCCGTGTCGTCAGATCTCAGGCTACTCAATTCCATGTA12840.12245610320646155No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT12800.12207462001890249No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12780.12188387842512295No Hit
GGTTTGTGCTGGTACCAGGCTAAGTAGTTGTGAGTAACAATCTGACTGGCCCTGCAGGAGACGGTGGCTCTTTCC12780.12188387842512295No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12700.1211209120500048No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12640.1205486872686662No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12430.11854590053398109No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC12310.11740145097130389No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTTTTCTTTGTGGTGGCAGCAG11990.11434958547083132No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11940.11387273148638248No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11800.11253754032992573No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11700.11158383236102806No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11600.11063012439213038No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC11590.11053475359524062No Hit
GCCCAATGTATGGTCCCGCAAAGTACGCACAGAAGTTCCAGGACAGAGTC11110.10595695534453178No Hit
CCTCATCTCTGGTGCATCCCGCAGGGCCACTGGCATCGCAGACAGGTTTACTGGCAGTGGGTCTGGGACAGACTT10930.10424028100051595No Hit
CAGTAAACCTGTCTGCGATGCCAGTGGCCCTGCGGGATGCACCAGAGATG10910.10404953940673642No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT10890.10385879781295687No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10660.10166526948449223No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC10640.1014745278907127No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10530.10042544912492524No Hit
GTATATTGCTGGCAGTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCT10490.10004396593736618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)