FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048539
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60310.5751812760421882No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34420.32826628289458No Hit
GTATTACTGCCAGCAATATACTACCTCAGGGAGAAATTTTGGCCAGGGGA31690.3022300553436734No Hit
GTGTATTACTGCCAGCAATATACTACCTCAGGGAGAAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA27810.26522618615044363No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25750.2455798019911515No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24580.23442141875504868No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21580.2058101796881184No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20490.19541476282713377No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20320.1937934592800077No Hit
CAGTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT17950.17119058041713278No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16830.1605090511654788No Hit
GTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGA16360.15602662371165973No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16160.1541192077738644No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16110.15364235378941554No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15730.1500182635076044No Hit
GTCCCAGACCCACTGCCAGTAAACCTGTCTGCGATGCCAGTGGCCCTGCG15500.14782473517913974No Hit
GTCTGGGGCTGAGGTGAAGAAGCCCGGGTCCTCGGTGAAGATCTCCTGCAAGGCTTCTGAAGTCAGCTTCAACAG15390.14677565641335227No Hit
GTCTCCAGGCTCCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCGTCTCCTGCAGGGCCAGTCAGATTGTTACTCA15340.14629880242890345No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14940.14248397055331277No Hit
CAACTACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCATCCCGCAGGGC14820.14133952099063554No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14790.14105340859996623No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCAGTAAACCTGTCTGCGATGCCA14520.13847839708394252No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14520.13847839708394252No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT14490.13819228469327322No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCGTCTCCTGCAGGGCCAGTCAGA14210.13552190238035972No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13950.13304226166122576No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13660.1302765085514225No Hit
GGTTTGTGCTGGTACCAGGCTAAGTAGTTGTGAGTAACAATCTGACTGGCCCTGCAGGAGACGGTGGCTCTTTCC13130.12522185631626484No Hit
ATATACTACCTCAGGGAGAAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT13120.12512648551937505No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTCTGCAGTGTA12930.12331444037846948No Hit
GCAATATACTACCTCAGGGAGAAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT12880.12283758639402063No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCATCCCGCA12830.12236073240957179No Hit
TCTCTGGTGCATCCCGCAGGGCCACTGGCATCGCAGACAGGTTTACTGGCAGTGGGTCTGGGACAGACTTCACT12540.11959497929976853No Hit
GGTTTACTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGA12310.11740145097130389No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12220.11654311379929597No Hit
CTCCAGGCTCCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCGTCTCCTGC12180.1161616306117369No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12150.1158755182210676No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12040.11482643945528016No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11950.11396810228327225No Hit
GGTTTACTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTCTGCAGTGT11770.11225142793925642No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11630.11091623678279969No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11620.11082086598590993No Hit
CAATAATAGACGGCCGTGTCGTCAGATCTCAGGCTACTCAATTCCATGTA11440.1091041916418941No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11440.1091041916418941No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC11260.1073875172978783No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT11140.10624306773520106No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC10850.10347731462539782No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAATTT10830.10328657303161827No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10790.1029050898440592No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10680.10185601107827176No Hit
CCTCATCTCTGGTGCATCCCGCAGGGCCACTGGCATCGCAGACAGGTTTACTGGCAGTGGGTCTGGGACAGACTT10640.1014745278907127No Hit
CAGTAAACCTGTCTGCGATGCCAGTGGCCCTGCGGGATGCACCAGAGATG10630.10137915709382292No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)