FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636568
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT76380.4667083799756564No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC65770.40187758773237653No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGATTCCA55640.3399797625274355No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAAGGCCAGTCAGAGTGTCAGCAA50570.30900029818498226No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49590.30301215714837393No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49230.3008124318696198No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT48690.29751284395148875No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45800.2798539382414907No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG42280.2583455132936731No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGAATGCCAGTGGCCCTGTT40910.24997433653841453No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39590.24190867718298292No Hit
CTCCCAGACTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATTCCA39400.24074771106364048No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAAC37360.22828260115070076No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37170.22712163503135832No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35150.21477873207834935No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34190.2089127980016718No Hit
CTCCTGCAAGGCCAGTCAGAGTGTCAGCAACTACTTAGCCTGGTTCCAACAGAAACCTGGCCAGGCTCCCAGACT33660.20567431356350607No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAAGGCCAGTCAGAGTGTCAGCAACTACTTAGCCTGGTT33550.20500217528388676No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32160.1965087915686974No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGCCTACTTTCGGCGGAGGGAC32090.19608106720893967No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31980.1954089289293204No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT31860.19467568716973568No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31700.19369803149028944No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31010.18948189137267746No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGG29510.18031636937786882No Hit
GTCTGGGAGCCTGGCCAGGTTTCTGTTGGAACCAGGCTAAGTAGTTGCTG29440.17988864501811108No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29440.17988864501811108No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTTGTCC29250.17872767889876864No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGAATGCCA29130.17799443713918395No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC28530.1743282283412605No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATTCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT27460.16779015598496366No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA26870.1641850506670056No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26800.16375732630724785No Hit
CCTTTGTCCCTCCGCCGAAAGTAGGCCAGTTGCTACGCTGCTGACAGTAA26760.1635129123873863No Hit
GTAGTAGACGGCCGCGTCCTCAGATGTCAGGCTGCTCAGCTCCATGTAGG26280.16057994534904751No Hit
GCGTAGCAACTGGCCTACTTTCGGCGGAGGGACAAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT26170.1599078070694282No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25380.15508063215216233No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24900.15214766511382358No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24750.15123111291434269No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24130.14744269715648844No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23780.14530407535769976No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23680.14469304055804585No Hit
GTCTACTACTGTGCCAGTAGCGGGGGGATTTTTGGGGCAGTTTCGTGGAAGAATCCCAGAAGCATTACCTTTGA23660.1445708335981151No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23640.1444486266381843No Hit
GTGTTGACACAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGC23500.14359317791866882No Hit
GTCAGAGTGTCAGCAACTACTTAGCCTGGTTCCAACAGAAACCTGGCCAGGCTCCCAGACTCCTCATCTATGATG23200.1417600735197071No Hit
GGACAAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC21920.13393882808413704No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21680.13247234456496765No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21660.13235013760503686No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTTGTCCCTCCGCCGAAAGTA20860.1274618592078056No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20760.1268508244081517No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20560.1256287548088439No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20500.12526213392905153No Hit
CAACAGGGCCACTGGCATTCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG20330.12422337476963989No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19820.12110709729140492No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC19540.119396199852374No Hit
GAGCCACCCTCTCCTGCAAGGCCAGTCAGAGTGTCAGCAACTACTTAGCCTGGTTCCAACAGAAACCTGGCCAGG19410.11860185461282391No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGATTC19170.11713537109365453No Hit
TTATTACTGTCAGCAGCGTAGCAACTGGCCTACTTTCGGCGGAGGGACAAAGGTGGAGATCAAACGAACTGTGG18780.11475233537500429No Hit
CTACTTAGCCTGGTTCCAACAGAAACCTGGCCAGGCTCCCAGACTCCTCATCTATGATGCATCCAACAGGGCCA18770.11469123189503888No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18590.11359136925566185No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18320.11194157529659629No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18300.11181936833666552No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18020.11010847089763455No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18020.11010847089763455No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17930.10955853957794605No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17770.10858088389849979No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT17710.10821426301870743No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17540.1071755038592958No Hit
CCTCATCTATGATGCATCCAACAGGGCCACTGGCATTCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT17520.10705329689936502No Hit
GCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGAATGCCAGTGG17470.10674777949953805No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17320.10583122730005719No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17170.10491467510057634No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGATTCCACCGGAGAAATTGTGTTGACACAGTCTC16860.10302046722164922No Hit
ACCCTCTCCTGCAAGGCCAGTCAGAGTGTCAGCAACTACTTAGCCTGGTT16860.10302046722164922No Hit
GAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTTCTCTGGCTC16840.10289826026171843No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16720.10216501850213372No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16720.10216501850213372No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16430.10039301758313739No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16390.10014860366327583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12500.046.46671
GTGGTAT2900.038.879341
GTATCAA32900.038.6322481
CGTATGC3500.038.4735741
TCGTATG3450.038.00336540
CGCGGTT9300.036.6921170
TGGTATC3300.034.15732
CTTATAC4850.033.814461
ACGAGAC4500.033.41662222
CACGAGA4700.032.71978821
ATCTCGT3850.032.17323737
AATCTCG3850.032.14652636
GGCAATC3950.032.131933
TATCAAC39650.032.046692
GTATGCC4550.031.90904442
ATCAACG39900.031.84593
ATGCCGT4250.031.78227244
TCTCCGA4850.031.69330611
TATACAC5400.031.6271253
GTATAAC553.4484276E-431.0606271
Produced by FastQC (version 0.11.5)