FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636568
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT78350.47874576552883835No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54720.3343582423706195No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGATTCCA53270.3254982377756378No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC50440.3082059529454321No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAAGGCCAGTCAGAGTGTCAGCAA49080.299895879670139No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT48990.2993459483504505No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48110.29396884211349605No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46930.2867586314775799No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44480.2717882788860591No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41400.2529684070567187No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGAATGCCAGTGGCCCTGTT39640.24221419458280985No Hit
CTCCCAGACTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATTCCA35720.2182616304363766No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAAC35680.21801721651651504No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35410.21636742255744948No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT34130.20854617712187945No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAAGGCCAGTCAGAGTGTCAGCAACTACTTAGCCTGGTT33340.20371900220461356No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32920.20115265604606714No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGCCTACTTTCGGCGGAGGGAC31260.19100947837181223No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT31080.1899096157324352No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30910.18887085657302355No Hit
CTCCTGCAAGGCCAGTCAGAGTGTCAGCAACTACTTAGCCTGGTTCCAACAGAAACCTGGCCAGGCTCCCAGACT30850.18850423569323121No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30430.18593788953468476No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30370.18557126865489243No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGG30250.18483802689530773No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30210.1845936129754462No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTTGTCC29470.18007195545800725No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29260.17878878237873402No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28710.17542809098063752No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28380.17341167614177963No Hit
GTCTGGGAGCCTGGCCAGGTTTCTGTTGGAACCAGGCTAAGTAGTTGCTG28170.17212850306250643No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGAATGCCA28170.17212850306250643No Hit
CCTTTGTCCCTCCGCCGAAAGTAGGCCAGTTGCTACGCTGCTGACAGTAA27760.1696232603839254No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC27430.16760684554506747No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27380.16730132814524054No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA27080.1654682237462788No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26730.1633296019474901No Hit
GTAGTAGACGGCCGCGTCCTCAGATGTCAGGCTGCTCAGCTCCATGTAGG26460.16167980798842455No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATTCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT25450.15550835651192008No Hit
GCGTAGCAACTGGCCTACTTTCGGCGGAGGGACAAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT25430.1553861495519893No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24550.1500090433150349No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24040.14689276583679994No Hit
GTGTTGACACAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGC23970.1464650414770422No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23810.14548738579759593No Hit
GTCTACTACTGTGCCAGTAGCGGGGGGATTTTTGGGGCAGTTTCGTGGAAGAATCCCAGAAGCATTACCTTTGA23420.1431043500789457No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23020.14066021088033007No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21580.13186130976531374No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA21370.13057813668604054No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21290.13008930884631742No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21070.12874503228707881No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20960.1280728940074595No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20830.12727854876790945No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTTGTCCCTCCGCCGAAAGTA20700.12648420352835935No Hit
GGACAAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC20600.12587316872870544No Hit
GTCAGAGTGTCAGCAACTACTTAGCCTGGTTCCAACAGAAACCTGGCCAGGCTCCCAGACTCCTCATCTATGATG20240.12367344344995136No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20190.12336792605012442No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19520.11927399289244321No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18880.11536337017465818No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGATTC18630.11383578317552341No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC18540.11328585185583488No Hit
GCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGAATGCCAGTGG18460.11279702401611176No Hit
CAACAGGGCCACTGGCATTCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG18430.11261371357621561No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18090.11053619525739229No Hit
TTATTACTGTCAGCAGCGTAGCAACTGGCCTACTTTCGGCGGAGGGACAAAGGTGGAGATCAAACGAACTGTGG18060.11035288481749614No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC17880.10925302217811908No Hit
GAGCCACCCTCTCCTGCAAGGCCAGTCAGAGTGTCAGCAACTACTTAGCCTGGTTCCAACAGAAACCTGGCCAGG17820.10888640129832675No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17730.10833646997863823No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17650.10784764213891508No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17650.10784764213891508No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17420.1064422620997111No Hit
GGGGAAAGAGCCACCCTCTCCTGCAAGGCCAGTCAGAGTGTCAGCAACTACTTAGCCTGGTTCCAACAGAAAC17280.10558681338019563No Hit
CTACTTAGCCTGGTTCCAACAGAAACCTGGCCAGGCTCCCAGACTCCTCATCTATGATGCATCCAACAGGGCCA17030.10405922638106084No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16890.10320377766154537No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16780.10253163938192607No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG16500.10082074194289513No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16470.10063743150299895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTT9650.047.7313470
GGTATCA13650.046.2226031
GTGGTAT3200.045.828311
TGGTATC3600.041.6823542
GTATCAA35450.038.674541
AATGGTA4550.036.3803970
ATCAACG41450.032.5003433
TATCAAC42000.032.236162
AACGCAG42400.032.0154156
CAACGCA42300.032.010475
TCAACGC42150.031.9635264
GAGTTTA4900.031.3207241
ACGCAGA44150.030.8236527
CGCAGAG44550.030.6234538
AGAGTAC44550.030.53942511
CGCGTCC7300.030.36634613
CCGCGTC7450.030.20440512
CAGAGTA45750.029.96748510
GCGTCCT7400.029.96331814
GCCGCGT7750.029.47873311
Produced by FastQC (version 0.11.5)