FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867626
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37330.43025451058405345No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35360.4075488747455701No Hit
CTTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTATTGGAGTTGAATAAAACACT35280.4066268184678652No Hit
CTCCTAAAGTGATCATTTACTGGGCATCTTCCCGGGAATTTGGGGTCCCT33980.3916434039551604No Hit
ATATTATATTACCCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT30790.3548764098816771No Hit
ATATTACCCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA28720.3310182036960626No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25380.2925223541018826No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24570.28318653429012036No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24000.2766168833114729No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22820.26301655321532547No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21170.24399914248766172No Hit
GGGTAATATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT20880.24065668848098146No Hit
ATATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC20670.23823629075200606No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20380.23489383674532577No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20240.23328023825934216No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20080.23143612570393235No Hit
GTTTATTACTGTCAGCAATATTATATTACCCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA20040.2309750975650799No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18860.21737476746893244No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18470.21287974311512103No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17970.20711689137946537No Hit
AACCAGGACAGCCTCCTAAAGTGATCATTTACTGGGCATCTTCCCGGGAA16880.19455387459573595No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAGCCAGAGTGTTTTATT15850.18268240002028524No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15740.181414572638441No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15480.17841788973590003No Hit
GTTTTATTCAACTCCAATAATAAGAACTACTTAGCTTGGTACCAGCAGAA15410.17761109049290824No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14410.16608538702159686No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA14190.16354973225790836No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATATTACC14010.1614751056330723No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCA13700.1579021375569658No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13700.1579021375569658No Hit
GTGTTGCAGACCCAGGTCCTCATTTCTCTGCTGCTCTGGATCTCTGGTGC13500.1555969968627035No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13470.15525122575856418No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13320.15352237023786747No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13290.1531765991337281No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12990.14971888809233472No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG12950.14925785995348226No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12830.1478747755369249No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12670.14603066298151507No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC12160.14015255421114628No Hit
GTCTCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAGCCAGAGTGTTTTATTCAACTCCAATAATAAGAACTA12030.1386542127598758No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11880.1369253572391791No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAAGTGATCA11730.13519650171848238No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11670.1345049595102037No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11570.13335238916307257No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAGC11350.13081673439938407No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11280.13000993515639228No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11070.12758953742741688No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11060.12747428039270378No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11020.12701325225385132No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTATTGGAGTTG10860.1251691396984415No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10780.12424708342073658No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCCTCATTTCTCTGCT10760.12401656935131036No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10750.12390131231659723No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10750.12390131231659723No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10600.12217245679590054No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10550.12159617162233498No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10520.12125040051819563No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT10390.11975205906692514No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAAGTGATCATT10290.11859948871979402No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10190.11744691837266288No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10110.11652486209495798No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10010.11537229174782683No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGTGGGGTAAT9980.11502652064368749No Hit
GCTCTGGATCTCTGGTGCCTGCGGGGACATCGTGATGACCCAGTCTCCAG9920.11433497843540881No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATATTACCCCACTCACTTT9910.11421972140069568No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9840.1134129221577039No Hit
GGATCTCTGGTGCCTGCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG9740.11226035181057276No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATATTACCCCAC9590.11053149628987606No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9520.10972469704688426No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC9440.10880264076917935No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9420.10857212669975314No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9390.10822635559561379No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9330.1075348133873351No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA9070.10453813048479413No Hit
TTATTACTGTCAGCAATATTATATTACCCCACTCACTTTCGGCGGAGGGA9060.10442287345008103No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9050.10430761641536791No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT9010.10384658827651545No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC8920.10280927496409743No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8910.10269401792938433No Hit
TTTCTCTGCTGCTCTGGATCTCTGGTGCCTGCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGT8890.10246350385995809No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8870.10223298979053187No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8850.10200247572110564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8800.043.43261
GTATCAA21600.037.1274031
TCGTATG2000.034.5075540
CGAGACG2250.033.3730423
TATCAAC23800.032.9690322
ATCAACG23800.032.9690323
CCTACGT450.003334796532.78107567
CGTGCAA651.9898578E-532.570857
TGGTATC2650.032.1846962
TCAACGC24400.032.1583184
CAACGCA24350.032.0842445
CGTATGC2050.032.0068641
TATACAC2350.031.9381773
CTTATAC2250.031.8505731
AACGCAG24750.031.703556
GTGGTAT2700.031.597791
ACGGGTC1307.2759576E-1231.49148816
ATCTCCG2500.031.38832310
TTATACA2400.031.2727972
TAACGGC553.3421224E-431.22488636
Produced by FastQC (version 0.11.5)