FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372425
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC65140.47463431517204946No Hit
ATATATGGGTCGTGGCATCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC59200.4313532615625626No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41960.3057361968777893No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG39360.28679162795781193No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGATGCCACGACCCATATATAGAACA34890.2542215421607738No Hit
CCCATATATAGAACACAATAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTC34520.2515255842760078No Hit
GTAGATGAGCGTGCGTGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGG33260.2423447547224803No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC33030.24066888901032843No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32510.23687997522633292No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGATGCCACGACCCATATATAGAACACAATAATAAT31140.22689764468003717No Hit
GTAATACACAGCCGTGTCCTCATCTCTCAGGCTGTTCATTTGCACATACA30630.22318159462265696No Hit
CCCCAGAAGAGCGAGTGTTTGTGCTGTAGATGAGCGTGCGTGGAGCCTGG30440.2217971838169663No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30190.21997559065158387No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA28950.21094048855128697No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28390.20686011986083028No Hit
GTAGAGACTGAGCCAGAGCTCAAGTCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGGCTC28330.20642293750113852No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT27470.20015665701222288No Hit
GGATGATGCTTCTCCTCGGACTCCTTCCTTATGGATCAGGAGTGGGTTCTCAGCCTGTGGTGACCCAGGAGCCAT25760.18769695976100698No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG25440.18536532050931745No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG25070.18266936262455144No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC24480.17837040275424887No Hit
CTCATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCGTTGGGAACAAAG24310.1771317194017888No Hit
CTATATATGGGTCGTGGCATCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCC23910.17421717033717687No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23610.17203125853871795No Hit
GATCAGGAGTGGGTTCTCAGCCTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACA22730.16561925059657176No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC22650.16503634078364937No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT22350.16285042898519045No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG22240.16204892799242218No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21850.15920724265442557No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21810.1589157877479644No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21350.15556405632366066No Hit
GCCTAAGAACACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGAT20820.15170227881304987No Hit
GTGTATTACTGTGCGAGAGATGATGGCTGGAGGTCCCGCTCAACCAACGA20750.15119223272674281No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20530.14958923074120625No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20180.14703900030967085No Hit
GTTCTATATATGGGTCGTGGCATCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG20120.14660181794997906No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19660.14325008652567536No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19230.14011694628121757No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTCCTTATGGATCAGGAGTGG19050.13880539920214222No Hit
GGAGTGGGTTCTCAGCCTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC18740.13654662367706796No Hit
GGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTGACCAGTAGAGACTGAG18710.13632803249722208No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC18290.13326775597937957No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT18120.13202907262691949No Hit
CTACTGGTCACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACA17570.12802156766307812No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGACTTGAGCTCTGG17550.12787584020984752No Hit
GTGATGGTGAGGGCAGCTTTGTTCCCAACGATGGAGCCAGAGAAGCGATC17460.12722006667030986No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17190.1252527460516968No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17110.12466983623877442No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCGTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGG16880.12299397052662259No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16670.12146383226770133No Hit
CCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACACTCGCTCT16670.12146383226770133No Hit
GACCAGTAGAGACTGAGCCAGAGCTCAAGTCACAAGTGAGTGTGACTGTC16380.11935078419585769No Hit
GCTTTGTTCCCAACGATGGAGCCAGAGAAGCGATCAGGGACCCCAGAAGA16210.11811210084339764No Hit
GCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTCCCAACGATGGAGCC16190.11796637339016704No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGATGCCACGAC16140.11760205475709055No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC16010.11665482631109168No Hit
CCCCTGGAGGGACAGTCACACTCACTTGTGACTTGAGCTCTGGCTCAGTC15820.11527041550540103No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTCCTTATGGATCAGGAGTGGGTT15660.11410459587955625No Hit
GTCTCTACTGGTCACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGC15560.11337595861340329No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15400.11221013898755852No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15160.11046140954879137No Hit
GGGCAGCTTTGTTCCCAACGATGGAGCCAGAGAAGCGATCAGGGACCCCA14890.10849408893017834No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14730.10732826930433356No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14620.1065267683115653No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT14470.10543381241233582No Hit
ATAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG14390.10485090259941346No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC14260.10390367415341459No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14040.10230067216787803No Hit
CCTTCCTTATGGATCAGGAGTGGGTTCTCAGCCTGTGGTGACCCAGGAGC13820.10069767018234148No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)