FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372425
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGGGTCGTGGCATCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC60820.44315718527424086No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC60460.44053409111609015No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39090.28482430733919883No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37390.27243747381459826No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG36430.26544255605952966No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34920.2544401333406197No Hit
CCCATATATAGAACACAATAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTC34610.25218135781554546No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGATGCCACGACCCATATATAGAACA34100.24846530775816528No Hit
GTAGATGAGCGTGCGTGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGG32050.23352824380202925No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGATGCCACGACCCATATATAGAACACAATAATAAT31390.2287192378454196No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC31190.22726196331311366No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29970.21837258866604733No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28150.20511139042206314No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28050.20438275315591017No Hit
CCCCAGAAGAGCGAGTGTTTGTGCTGTAGATGAGCGTGCGTGGAGCCTGG27750.20219684135745122No Hit
GGATGATGCTTCTCCTCGGACTCCTTCCTTATGGATCAGGAGTGGGTTCTCAGCCTGTGGTGACCCAGGAGCCAT27740.20212397763083595No Hit
GTAGAGACTGAGCCAGAGCTCAAGTCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGGCTC27160.19789788148714865No Hit
GTAATACACAGCCGTGTCCTCATCTCTCAGGCTGTTCATTTGCACATACA26840.19556624223545913No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26640.19410896770315317No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG26230.19112155491192598No Hit
CTCATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCGTTGGGAACAAAG26080.1900285990126965No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA26000.18944568919977411No Hit
CTATATATGGGTCGTGGCATCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCC24300.1770588556751735No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG23950.17450862524363808No Hit
GATCAGGAGTGGGTTCTCAGCCTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACA21780.15869719656811848No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21770.15862433284150318No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT21660.1578228318487349No Hit
GCCTAAGAACACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGAT21560.15709419458258192No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21020.15315955334535583No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC20860.15199373371951108No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20820.15170227881304987No Hit
GTTCTATATATGGGTCGTGGCATCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG20680.15068218664043573No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG20500.14937063956136037No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20340.1482048199355156No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT20070.14623749931690255No Hit
GTGTATTACTGTGCGAGAGATGATGGCTGGAGGTCCCGCTCAACCAACGA19940.14529027087090368No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19860.14470736105798132No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTCCTTATGGATCAGGAGTGG19820.1444159061515201No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19820.1444159061515201No Hit
GGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTGACCAGTAGAGACTGAG19600.14281290416598358No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGACTTGAGCTCTGG18790.13691094231014445No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCGTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGG18770.13676521485691387No Hit
GGAGTGGGTTCTCAGCCTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC18720.13640089622383736No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC18650.13589085013753027No Hit
CTACTGGTCACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACA18610.1355993952310691No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18570.1353079403246079No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC18140.1321748000801501No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17440.12707433921707925No Hit
GTGATGGTGAGGGCAGCTTTGTTCCCAACGATGGAGCCAGAGAAGCGATC17300.12605424704446508No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16860.12284824307339198No Hit
GCTTTGTTCCCAACGATGGAGCCAGAGAAGCGATCAGGGACCCCAGAAGA16380.11935078419585769No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15970.11636337140463048No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15920.11599905277155399No Hit
CCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACACTCGCTCT15830.11534327923201633No Hit
GTCTCTACTGGTCACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGC15810.11519755177878573No Hit
CCCCTGGAGGGACAGTCACACTCACTTGTGACTTGAGCTCTGGCTCAGTC15770.11490609687232455No Hit
GACCAGTAGAGACTGAGCCAGAGCTCAAGTCACAAGTGAGTGTGACTGTC15570.11344882234001859No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC15430.11242873016740441No Hit
GCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTCCCAACGATGGAGCC15110.11009709091571489No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTCCTTATGGATCAGGAGTGGGTT14870.10834836147694774No Hit
GGGCAGCTTTGTTCCCAACGATGGAGCCAGAGAAGCGATCAGGGACCCCA14680.10696395067125708No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGATGCCACGAC14630.1065996320381806No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT14490.10557953986556642No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC14340.10448658396633695No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14130.10295644570741572No Hit
CATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCGTTGGGAACAAAGCTGC14060.10244639962110863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12000.043.572281
GTGGTAT3200.043.501551
TCTGGAG11850.037.94887570
TGGTATC3750.037.119972
GTATCAA25450.033.218681
CAGGGAA10600.032.6873370
AGTTTAT3600.031.1219232
AATACGT450.00499748530.18213710
ATACGTA450.00500283330.17553711
GACAGGT2050.029.8118881
GAGTTTA3900.029.5995521
TCAACGC28450.028.8857144
ATCAACG28550.028.7824383
TATCAAC29050.028.7504042
AACGCAG29200.028.4905996
GTATAAA605.9548067E-428.293691
CAACGCA29250.028.2097195
TTATTCA4150.027.819495
TGATTGA1104.2815373E-827.7782482
TATTCAG4200.027.4883046
Produced by FastQC (version 0.11.5)