FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423905
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATAGTGTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT24830.5857444474587467No Hit
CTCCTAAACTGCTCATTAACTGGGCATCTACCCGGGAATCCGGGGTCCCT21850.5154456776872177No Hit
GTATTACTGTCAGCAATACTATAGTGTTCCGTACACTTTTGGCCAGGGGA20660.487373350161003No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17390.4102334249419091No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT16720.39442799683891433No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15130.35691959283329994No Hit
CTATAGTATTGCTGACAGTAATACACTGCCACATCTTCAGCCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC14990.35361696606550996No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCCAAGTAGTTCTTT14550.3432372819381701No Hit
GTGTATTACTGTCAGCAATACTATAGTGTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA13670.32247791368349044No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11880.280251471438176No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11340.2675127681909862No Hit
GTTTTATTCAGCCCCAACAGCCCCCAGAAAAAGAACTACTTGGCTTGGTA10670.25170734008799145No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9850.2323633833052217No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA9200.2170297590261969No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9130.21537844564230194No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8850.20877319210672202No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8510.20075252709923214No Hit
GTATTACTGTACAGCGAGCCTTGGAGGGGTTTGGGGAGTCTTTGATAACTGGGGCCAGGGAACCCTGGTCACCGT8390.1979217041554122No Hit
GCTCATTAACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTT7940.18730611811608733No Hit
ATAGTGTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA7920.18683431429211733No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7890.18612660855616234No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7780.18353168752432739No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7640.18022906075653744No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7470.17621872825279247No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7310.17244429766103256No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7160.1689057689812576No Hit
AACCAGGACAGCCTCCTAAACTGCTCATTAACTGGGCATCTACCCGGGAA7160.1689057689812576No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7070.16678265177339263No Hit
GTAATACAGGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATACA6920.1632441230936177No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC6780.15994149632582771No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC6710.15829018294193276No Hit
CAGTAATACACTGCCACATCTTCAGCCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCG6590.15545935999811278No Hit
CAGTAATACAGGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATA6480.15286443896627783No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6430.15168492940635284No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCAGCCAGAGTGTTTTATTCAGCCCCAA6410.15121312558238284No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCAGCCAGAGTGTTTTATT6370.15026951793444285No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6340.14956181219848788No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6250.1474386949906229No Hit
GCCCCAACAGCCCCCAGAAAAAGAACTACTTGGCTTGGTACCAGCAGAAA6250.1474386949906229No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGGCTGAAGATGTGGCAGTGTATTACTGTCAGCAATACTATAGTGTT6240.1472027930786379No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6100.14390016631084795No Hit
CTATAGTGTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT6060.14295655866290796No Hit
CCCTTAGAGTCTCCTGTGCAGCCTCTGGAGCCACCATCAGTAACACCTGT5830.13753081468725303No Hit
GAATAAAACACTCTGGCTGGACTTGCAGTTGATGGAGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA5740.13540769747938808No Hit
GTCCCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCAC5660.1335204821835081No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5640.1330486783595381No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG5620.13257687453556813No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5360.1264434248239582No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5350.1262075229119732No Hit
GCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGA5320.1254998171760182No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA5310.12526391526403322No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG5160.12172538658425827No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5040.11889456364043831No Hit
GTTAATGAGCAGTTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCCA5020.11842275981646831No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCAGC4920.11606374069661833No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT4910.11582783878463333No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT4860.11464832922470837No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG4800.1132329177527984No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT4790.1129970158408134No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG4700.11087389863294841No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG4630.10922258524905344No Hit
GTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCAGCCAGAGTGTTT4610.10875078142508345No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC4590.10827897760111345No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT4560.10757127186515845No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA4510.10639176230523349No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG4430.10450454700935352No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC4320.10190962597751854No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG4310.10167372406553354No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG4310.10167372406553354No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT4300.10143782215354855No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA4290.10120192024156355No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG4290.10120192024156355No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGTC150.002276145469.591245
CGTATGG208.444647E-569.10383641
GCGTATG150.00236297868.9373640
CCTAACG150.002567963467.5062562
CTAACGC150.002567963467.5062563
TACTAGG150.002567963467.5062562
ATGTCGT454.638423E-1060.9108137
GTCGTAT401.2143573E-860.24037639
TGTCGTA401.2352757E-860.11021838
TATGGCG252.4965365E-455.50423443
TAGTATG252.5768025E-455.1498940
ATGCCGT3600.054.9927344
CCGTCTT3500.054.89238747
GCCGTCT3450.054.5821446
TCGTATG3550.054.3731340
CGTATGC3500.054.29587641
TAGCGCT4400.053.95910329
CTAGCGC4400.053.8760928
CTTGAAA3750.053.34300257
TATACAC4650.052.9887733
Produced by FastQC (version 0.11.5)