FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423905
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41380.9761621117939162No Hit
ATACTATAGTGTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT26010.6135808730729763No Hit
CTATAGTATTGCTGACAGTAATACACTGCCACATCTTCAGCCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC25700.6062679138014414No Hit
GTATTACTGTCAGCAATACTATAGTGTTCCGTACACTTTTGGCCAGGGGA19710.4649626685224284No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16930.3993819369905993No Hit
CTCCTAAACTGCTCATTAACTGGGCATCTACCCGGGAATCCGGGGTCCCT15000.35385286797749493No Hit
CAGTAATACACTGCCACATCTTCAGCCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCG14360.33875514561045517No Hit
GTGTATTACTGTCAGCAATACTATAGTGTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA13050.3078519951404206No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCCAAGTAGTTCTTT12940.30525707410858566No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11320.2670409643670162No Hit
GTAATACACTGCCACATCTTCAGCCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCG10380.24486618464042653No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10180.24014814640072657No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10140.23920453875278658No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10040.23684551963293662No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9460.22316320873780682No Hit
GTATTACTGTACAGCGAGCCTTGGAGGGGTTTGGGGAGTCTTTGATAACTGGGGCCAGGGAACCCTGGTCACCGT8760.20665007489885706No Hit
ATAGTGTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA7970.18801382385204232No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7970.18801382385204232No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA7530.17763413972470246No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7310.17244429766103256No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7270.17150069001309257No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7180.16937757280522758No Hit
GAACACTATAGTATTGCTGACAGTAATACACTGCCACATCTTCAGCCTGCAGACTGCTGATGGTGAGAGTGAAAT7180.16937757280522758No Hit
GTTTTATTCAGCCCCAACAGCCCCCAGAAAAAGAACTACTTGGCTTGGTA6770.15970559441384272No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6730.15876198676590272No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC6650.15687477147002277No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6630.15640296764605277No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6550.15451575235017279No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6410.15121312558238284No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6360.15003361602245785No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGGCTGAAGATGTGGCAGTGTATTACTGTCAGCAATACTATAGTGTT6110.14413606822283295No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC5780.13635130512732804No Hit
GGCCAAAAGTGTACGGAACACTATAGTATTGCTGACAGTAATACACTGCC5780.13635130512732804No Hit
GTAATACAGGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATACA5750.13564359939137308No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5680.1339922860074781No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG5570.13139736497564314No Hit
CCCTTAGAGTCTCCTGTGCAGCCTCTGGAGCCACCATCAGTAACACCTGT5460.12880244394380816No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5460.12880244394380816No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5450.12856654203182316No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCGCTGAATCGGTCAGGGACCCCG5420.12785883629586817No Hit
GCTCATTAACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTT5310.12526391526403322No Hit
CTATAGTGTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT5160.12172538658425827No Hit
GTCCCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCAC5090.12007407320036328No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC4980.11747915216852832No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT4960.11700734834455832No Hit
CAGTAATACAGGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATA4930.11629964260860333No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG4890.11535603496066336No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG4860.11464832922470837No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT4840.11417652540073837No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCAGCCAGAGTGTTTTATT4840.11417652540073837No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA4740.1118175062808884No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCAGCCAGAGTGTTTTATTCAGCCCCAA4730.11158160436890341No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA4680.11040209480897842No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT4660.10993029098500844No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC4650.10969438907302344No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA4610.10875078142508345No Hit
GTCCCAGACCCGCTGCCGCTGAATCGGTCAGGGACCCCGGATTCCCGGGT4590.10827897760111345No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG4530.10686356612920349No Hit
GGACTGGAGTGGGTTGGCCGTATTAAGAGCAACACTGATGGTGGGACAAC4490.1059199584812635No Hit
AACCAGGACAGCCTCCTAAACTGCTCATTAACTGGGCATCTACCCGGGAA4430.10450454700935352No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG4420.10426864509736852No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA4380.10332503744942853No Hit
GCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGA4350.10261733171347354No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA4340.10238142980148854No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAACA4260.10049421450560857No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)