FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022690
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44210.43229131017219297No Hit
CTCATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA36850.3603242429279645No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36750.3593464295143201No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32630.31906051687217046No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32270.3155403885830506No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31050.3036110649365888No Hit
CTATTATACTGGTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC30190.2952018695792469No Hit
GTATAATAGTTATTTCCTGTCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT29040.2839570153223362No Hit
GAGTATGAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGT26410.2582405225434882No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26380.2579471785193949No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25840.25266698608571514No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25440.24875573243113752No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC25090.24533338548338207No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24930.243768884021551No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24540.2399554117083378No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24080.23545747000557354No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22880.22372370904184063No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20950.20485191015850354No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20130.19683384016661942No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19920.19478043199796616No Hit
CCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTCATACTCTGA19670.19233589846385513No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19440.190086927612473No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA19300.18871798883337082No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18130.17727757189373122No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17950.1755175077491713No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17720.17326853689778918No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17690.17297519287369584No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG17620.17229072348414476No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17490.17101956604640703No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTC17450.17062844068094926No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17040.1666194056850072No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16820.1644682161749895No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG15820.15469008203854542No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15670.15322336191807878No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15490.15146329777351886No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15310.14970323362895893No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG15230.1489209828980434No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15040.14706313741211902No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14890.1455964172916524No Hit
TACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCA14650.14324966509890583No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC14630.14305410241617694No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14560.14236963302662586No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATGAGTAGCTGGTTGGCCTGGTATCAGCAGAAA14560.14236963302662586No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14410.14090291290615925No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14340.14021844351660817No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT14260.13943619278569264No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14220.13904506742023487No Hit
GTTATTTCCTGTCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA14170.13855616071341267No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG14120.13806725400659048No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14110.13796947266522602No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT13980.1366983152274883No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13880.13572050181384387No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13800.13493825108292834No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA13520.132200373524724No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13430.13132034145244403No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13360.13063587206289295No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13320.1302447466974352No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA13060.12770243182195973No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12980.1269201810910442No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12830.1254534609705776No Hit
GCTGATACCAGGCCAACCAGCTACTCATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATG12650.12369339682601767No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC12630.12349783414328877No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12460.12183555134009329No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12460.12183555134009329No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12200.11929323646461781No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATGAGTAG12160.11890211109916006No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12140.11870654841643116No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC12120.11851098573370229No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12000.117337609637329No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11940.11675092158914237No Hit
TTCTAAACTAGACGCCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT11530.11274188659320028No Hit
GGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC11020.1077550381836138No Hit
TTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC10990.10746169415952048No Hit
GGTTTCTGCTGATACCAGGCCAACCAGCTACTCATACTCTGACTGGCCCGGCAAGTGATGGTGACT10950.10707056879406271No Hit
GATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGC10530.10296375245675621No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG10440.10208372038447625No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10370.10139925099492515No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10350.10120368831219627No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT10330.10100812562946737No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10320.10091034428810294No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10280.10051921892264518No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)