FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749605
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC68950.3940889515061971No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTGGGTGCCTCCGCCGAACACAGCGACACTCAGGCTGCTATCCCATGTT62640.3580236681993936No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCGTAAGT37690.21542005195458402No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTGGGTGCCTC36610.20924723008907725No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT35880.20507485975405876No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATGATGTATCCTGGTACCAGCAG33800.1931864620871568No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG30900.17661129226311081No Hit
CCCAGGAACAGCCCCCAAATTCCTCATTTATGACAATGATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGC30600.17489661952269228No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCT30290.17312479102425976No Hit
CTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG30040.17169589707391097No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29200.16689481340073903No Hit
GTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG28010.16009327819707875No Hit
GTAATACAAGGCGGTGTCCTCAATTCTCAGACTGTTCATTTGTAAATCGA28000.16003612243906482No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26880.15363467754150223No Hit
GTTTGGTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAG25880.14791910174010706No Hit
GTACTAGCACATTCTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCC25140.14368957564707463No Hit
GGCTCAGGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGA25030.14306086230892115No Hit
GATTATTACTGCGGAACATGGGATAGCAGCCTGAGTGTCGCTGTGTTCGG24790.1416891241165863No Hit
CCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG23790.13597354831519115No Hit
GGGCTGGATGACTTGGGATGGGGGCAGAGACCCCTCCCCTGGGAACCTGC23650.13517336770299582No Hit
GTTCCTGGGAGCTGCTGGTACCAGGATACATCATTATTCCCAATGTTGGA23630.1350590561869679No Hit
GATGTATCCTGGTACCAGCAGCTCCCAGGAACAGCCCCCAAATTCCTCAT23000.13145824343208895No Hit
ACACTGGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC22620.1292863246275588No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGGC22580.12905770159550298No Hit
GGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22520.12871476704741927No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGGAGG21910.12522826580856822No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC21880.12505679853452636No Hit
CAGTAATACAAGGCGGTGTCCTCAATTCTCAGACTGTTCATTTGTAAATC21790.12454239671240079No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC21610.12351359306814967No Hit
TGATTATACCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGTCTGGAGT21610.12351359306814967No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21540.12311350276205199No Hit
GGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGGGATAGCAGCCTGAGTGTCG21420.12242763366588458No Hit
GGATATTTATTGAGAAGGGCTGGATGACTTGGGATGGGGGCAGAGACCCCTCCCCTGGGAACCTGCAGCTCCAGG21060.12037002637738231No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCGTAAGTGACTTCTACC20660.11808379605682424No Hit
GTATTACTGTGCAAAAGATATCTTCGACTACAAATATGCTTACAGACTTGACTACTGGGGCCAGGGAACCCTGGT20580.11762654999271265No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT20290.11596903301030805No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG19830.11333986814166626No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTCCAACTGGTGGAGT19770.11299693359358255No Hit
CTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATGATGTATCCTGGTACCAGCAGCTCCCAGGAACAGCCCC19470.111282260853164No Hit
GGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGACGGGG19110.10922465356466174No Hit
ATCCCATGTTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG19100.10916749780664778No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACGGCTC18800.10745282506622923No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT18650.10659548869601995No Hit
GGGTGGCTGACGTGCCAGACTTGGAGGCAGAGAATCGGTCAGGAATCCCT18610.10636686566396415No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCATACTT18470.10556668505176882No Hit
CCCCTCCCCTGGGAACCTGCAGCTCCAGGCCCCTGTGGGTGGGGTGAGGG18340.10482366019758746No Hit
ACATGGGATAGCAGCCTGAGTGTCGCTGTGTTCGGCGGAGGCACCCAGCTGACCGTCCTCGGTCAGCCCAAGG18130.10362338927929447No Hit
GGATACATCATTATTCCCAATGTTGGAGCTGCTTCCAGAGCAGGAGATGGTGACCTTCTGTCCTGGGGCCGCAGA18060.10322329897319682No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGG17930.10248027411901545No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG17870.10213733957093173No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTACGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17670.10099422441065269No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTGGGTG17520.1001368880404434No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG252.6884585E-454.6881436
AGGGGGG6700.054.3436270
GTGGTAT2600.046.881031
ATCGCGT306.561052E-445.64064837
GGTATCA13650.044.1525041
GTCGTAT1200.040.08622439
ACGCGTA9950.039.1643227
TATACAC10700.038.899233
CGCGTAG10050.038.79905328
CGTATGC9800.038.72311841
GACGCGT10100.038.5628126
TCGTATG9300.038.50187340
CTTATAC10750.038.4254841
GCGTAGT10200.038.248229
TCTTATA3650.038.011122
AGACGCG10250.037.98869325
CGAGACG10250.037.9713223
CGTAGTA10500.037.5150330
CACGAGA10700.037.32009521
ATGTCGT1200.037.08302737
Produced by FastQC (version 0.11.5)