FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749605
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG84790.4846236722002966No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC69690.39831847759922956No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTGGGTGCCTCCGCCGAACACAGCGACACTCAGGCTGCTATCCCATGTT56670.32390168066506436No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG41900.23948262607845772No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCGTAAGT35020.2001594645648589No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC34730.19850194758245432No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATGATGTATCCTGGTACCAGCAG32260.18438447535300825No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTGGGTGCCTC30590.17483946376467832No Hit
CCCAGGAACAGCCCCCAAATTCCTCATTTATGACAATGATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGC29560.1689524206892413No Hit
CTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG28660.16380840246798564No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCT28530.16306537761380427No Hit
GTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG27270.15586375210404635No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG25940.14826203628819076No Hit
GTAATACAAGGCGGTGTCCTCAATTCTCAGACTGTTCATTTGTAAATCGA25310.14466122353331182No Hit
GTTTGGTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAG25280.14448975625926996No Hit
GGCTCAGGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGA24390.13940289379602824No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24380.1393457380380143No Hit
GTACTAGCACATTCTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCC24300.13888849197390268No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24270.13871702469986083No Hit
GGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA24040.13740244226553994No Hit
GATTATTACTGCGGAACATGGGATAGCAGCCTGAGTGTCGCTGTGTTCGG23980.1370595077174562No Hit
GGGCTGGATGACTTGGGATGGGGGCAGAGACCCCTCCCCTGGGAACCTGC23570.13471612163888422No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGGAGG23230.13277282586640984No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC22450.1283146767413216No Hit
GGGTGGCTGACGTGCCAGACTTGGAGGCAGAGAATCGGTCAGGAATCCCT22330.1276288076451542No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT22050.12602844642076355No Hit
CCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG22050.12602844642076355No Hit
GGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTC21970.12557120035665192No Hit
GATGTATCCTGGTACCAGCAGCTCCCAGGAACAGCCCCCAAATTCCTCAT21440.12254194518191248No Hit
GGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGGGATAGCAGCCTGAGTGTCG21310.12179892032773112No Hit
GTATTACTGTGCAAAAGATATCTTCGACTACAAATATGCTTACAGACTTGACTACTGGGGCCAGGGAACCCTGGT21070.12042718213539627No Hit
TGATTATACCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGTCTGGAGT20910.11951269000717304No Hit
ACACTGGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20790.11882682091100562No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTCCAACTGGTGGAGT20330.11619765604236384No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC20230.11562609846222432No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGGC20140.11511169664009877No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCGTAAGTGACTTCTACC20040.11454013905995925No Hit
CAGTAATACAAGGCGGTGTCCTCAATTCTCAGACTGTTCATTTGTAAATC19910.11379711420577787No Hit
GGATATTTATTGAGAAGGGCTGGATGACTTGGGATGGGGGCAGAGACCCCTCCCCTGGGAACCTGCAGCTCCAGG19330.11048208024096867No Hit
GGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGACGGGG19250.11002483417685706No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGG19200.1097390553867873No Hit
GTTCCTGGGAGCTGCTGGTACCAGGATACATCATTATTCCCAATGTTGGA19190.10968189962877335No Hit
GGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGCCCAGT19010.10865309598452222No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC18920.10813869416239665No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCATACTT18910.10808153840438271No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACGGCTC18850.10773860385629899No Hit
CTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATGATGTATCCTGGTACCAGCAGCTCCCAGGAACAGCCCC18690.10682411172807578No Hit
GGGTGAGGGTCGGGGGCCTAAGAGCATTCTGCAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC18350.1048808159556014No Hit
GCTTCAGCTGTGAGCGCAGGAGGCAGGACTCGGGACAATCTTCATCATGA18150.10373770079532237No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT18030.10305183169915495No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCT17910.10236596260298754No Hit
ATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTG17750.1014514704747643No Hit
CTCCTGTGTAGCCTCTGGATTCGCCTTTGATGATTATACCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGT17560.1003655110724992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4050.043.3644261
GGTATCA15150.041.9112361
TGGTATC4800.035.8861272
GTATCAA31900.033.2448351
ATCGATA450.00510776530.0481623
TATCAAC36750.028.490632
AACGAAC1403.6379788E-1027.6988951
ATCAACG38050.027.6939683
TCAACGC38300.027.3360524
TCTATTG650.00821189927.26387269
CGACACG500.00858268827.0185996
CAACGCA39100.026.690375
GACAGGT3200.026.3861051
AACGCAG39800.026.3057966
AATGGTA4850.025.43165470
ACGCAGA41700.025.107217
GGATATT6550.024.750571
CGCAGAG42100.024.708228
AGAGTAC42550.024.60284411
CAGAGTA42650.024.54726210
Produced by FastQC (version 0.11.5)