FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1350663
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTACAGTATCCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAGGAACTGTGGCTGCACCAT51050.3779625265517749No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46770.3462743852463568No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40270.29814987158158623No Hit
GATCTATGGTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA38830.2874884408620063No Hit
GTCTCTGGTGTCTCCATCACCAGTAGAAGTCACTTGTGGGGCTGGATCCG38770.2870442145820238No Hit
CTGTAACTCTGTTGACAATAATAAGTTGCAAAATCTTCAGGTTGTAGATTGCTGATGGTGAGAGTGAAATCTGTC35210.2606867886363956No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGACCATTGGCACCTATTTAAATTGGTATCAACAGAGA32300.2391418140572445No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG30900.2287765341909862No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29420.2178189526180846No Hit
ATAATAGACAGCCATGTCAGCGGCGGTCACAGAGGCCAGCCTCAGGGAGACTTGATTCTTCGACGTGTCGGCGGA28310.2096007664384084No Hit
CCATAGATCAGGACATAAGGGGCTTTTCCTGGTCTCTGTTGATACCAATT26930.19938356199881097No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC26810.19849510943884596No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26200.19397880892569058No Hit
CAATAATAGACAGCCATGTCAGCGGCGGTCACAGAGGCCAGCCTCAGGGA24940.1846500570460581No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23960.17739436113967733No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23200.1717674949265657No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22780.1686579109666882No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG22560.16702908127341906No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22550.16695504356008864No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22340.1654002515801499No Hit
GTCTCCATCCTCCCTGTCTGGATCTGTAGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGACCATTGGCAC22200.16436372359352405No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22080.16347527103355908No Hit
ACTATAATCCGTCCCTCAAGACTCGAGTCACGATGTCCGCCGACACGTCGAAGAATCAAGTCTCCCTGAGGCTGG21970.1626608561869245No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21850.1617724036269595No Hit
CACCTGCACTGTCTCTGGTGTCTCCATCACCAGTAGAAGTCACTTGTGGG21780.1612541396336466No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21700.16066183792700328No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21320.15784840482044746No Hit
GGATTGGAGTGGATTGGCAATTTGCATTATGGCGGGAACACCTACTATAA21320.15784840482044746No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21270.15747821625379535No Hit
AGTCAGACCATTGGCACCTATTTAAATTGGTATCAACAGAGACCAGGAAA20590.15244365174732705No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20520.15192538775401415No Hit
GTCTGGATCTGTAGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGA20400.15103693519404915No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTTCGAGGGATA20270.15007444492075372No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20040.14837157751415417No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19560.14481776727429418No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19500.14437354099431168No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19430.14385527700099876No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT19270.1426706735877121No Hit
GATTTCACTCTCACCATCAGCAATCTACAACCTGAAGATTTTGCAACTTATTATTGTCAACAGAGTTACAGTATC18990.14059761761446046No Hit
CTATTATTGTGCGAGACAAGAATCAAATTTTGGCCCCAACTGGTTCGACCCGTGGGGCCAGGGAATTCTGGTCAC17860.1322313560081234No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC17520.12971407375488928No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17210.12741890464164635No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT17180.1271967915016551No Hit
CTTATGTCCTGATCTATGGTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGA16630.12312471726848222No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16230.12016320873526556No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA16190.11986705788194389No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16170.11971898245528308No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16170.11971898245528308No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15510.1148324933754756No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC15270.11305558825554561No Hit
GGACATAAGGGGCTTTTCCTGGTCTCTGTTGATACCAATTTAAATAGGTG15210.11261136197556311No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGAATTCCCTGGCCCCACGGGTCGAACCAGTTGGGGC14980.11090849456896353No Hit
GTCTATTATTGTGCGAGACAAGAATCAAATTTTGGCCCCAACTGGTTCGA14740.10913158944903356No Hit
CCCTGAGGCTGGCCTCTGTGACCGCCGCTGACATGGCTGTCTATTATTGTGCGAGACAAGAATCAAATTTTGGCC14730.10905755173570313No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14630.10831717460239897No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14360.10631815634247774No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14200.10513355292919106No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14180.10498547750253025No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13940.10320857238260024No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13930.10313453466926983No Hit
GTCCCTCACCTGCACTGTCTCTGGTGTCTCCATCACCAGTAGAAGTCACT13710.10150570497600067No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13610.10076532784269651No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13570.10046917698937484No Hit
GTCCTGATCTATGGTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTT13570.10046917698937484No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCTA100.008410313136.9510370
TACCGAC200.00759358351.3414316
TATCGCG950.050.70917536
GTATCGC1000.048.15041435
TTACCGT305.6465494E-447.0513360
TCGCGTT306.347017E-445.9481438
ATCGCGT900.045.92416837
GTGGTAT2800.044.0248681
CGCGTAT551.4783473E-743.87757539
AGTATCG1750.043.20656634
CTCTTAT1800.041.8508031
ACGAGAC5200.040.8204322
CGTATGC2500.040.05550841
ATCTCGT2500.039.9540337
GACCGAT5000.039.73640426
ACCGATC5050.039.35755527
TATACAC5600.038.5017973
CGAGACC5250.038.47529623
TCAGTAT5300.038.2269932
AGACCGA5200.038.19534325
Produced by FastQC (version 0.11.5)