FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004689292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1350663
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50010.37026260436541164No Hit
GAGTTACAGTATCCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAGGAACTGTGGCTGCACCAT48690.3604896262057967No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47290.35012434633953843No Hit
GATCTATGGTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA36630.27120014392931474No Hit
GTCTCTGGTGTCTCCATCACCAGTAGAAGTCACTTGTGGGGCTGGATCCG36400.26949727652271516No Hit
CTGTAACTCTGTTGACAATAATAAGTTGCAAAATCTTCAGGTTGTAGATTGCTGATGGTGAGAGTGAAATCTGTC33620.24891479221685942No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGACCATTGGCACCTATTTAAATTGGTATCAACAGAGA30550.22618521422442164No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29850.22100257429129253No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29670.219669895451345No Hit
ATAATAGACAGCCATGTCAGCGGCGGTCACAGAGGCCAGCCTCAGGGAGACTTGATTCTTCGACGTGTCGGCGGA29400.21767087719142378No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28380.2101190304317213No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27170.20116046711874097No Hit
CCATAGATCAGGACATAAGGGGCTTTTCCTGGTCTCTGTTGATACCAATT24360.18035586967289396No Hit
ACTATAATCCGTCCCTCAAGACTCGAGTCACGATGTCCGCCGACACGTCGAAGAATCAAGTCTCCCTGAGGCTGG23380.17310017376651318No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC23370.1730261360531828No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22920.16969443895331404No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22330.16532621386681948No Hit
CAATAATAGACAGCCATGTCAGCGGCGGTCACAGAGGCCAGCCTCAGGGA22290.16503006301349782No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21360.1581445556737691No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21240.1572561031138041No Hit
GTCTGGATCTGTAGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGA20810.1540724814405962No Hit
CACCTGCACTGTCTCTGGTGTCTCCATCACCAGTAGAAGTCACTTGTGGG19740.1461504461142417No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19740.1461504461142417No Hit
GTCTCCATCCTCCCTGTCTGGATCTGTAGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGACCATTGGCAC19680.1457062198342592No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19570.1448918049876246No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19450.1440033524276596No Hit
GGATTGGAGTGGATTGGCAATTTGCATTATGGCGGGAACACCTACTATAA19430.14385527700099876No Hit
GATTTCACTCTCACCATCAGCAATCTACAACCTGAAGATTTTGCAACTTATTATTGTCAACAGAGTTACAGTATC19200.14215240959439918No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTTCGAGGGATA19080.1412639570344342No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19070.14118991932110378No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18800.13919090106118254No Hit
AGTCAGACCATTGGCACCTATTTAAATTGGTATCAACAGAGACCAGGAAA18500.13696976966127006No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18470.13674765652127882No Hit
CCCTGAGGCTGGCCTCTGTGACCGCCGCTGACATGGCTGTCTATTATTGTGCGAGACAAGAATCAAATTTTGGCC18460.1366736188079484No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18410.13630343024129632No Hit
CTATTATTGTGCGAGACAAGAATCAAATTTTGGCCCCAACTGGTTCGACCCGTGGGGCCAGGGAATTCTGGTCAC18010.13334192170807968No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17890.13245346914811468No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17490.12949196061489804No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17350.1284554326282722No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC17300.1280852440616201No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16790.12430932068176889No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16750.1240131698284472No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16710.12371701897512553No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA16580.12275452870183012No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT16000.118460341328666No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15910.11779400190869226No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT15450.1143882670954931No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15450.1143882670954931No Hit
CTTATGTCCTGATCTATGGTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGA15280.11312962596887602No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14970.11083445685563312No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14940.11061234371564188No Hit
GTCTATTATTGTGCGAGACAAGAATCAAATTTTGGCCCCAACTGGTTCGA14640.10839121231572939No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14520.10750275975576438No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14330.10609604320248647No Hit
GGACATAAGGGGCTTTTCCTGGTCTCTGTTGATACCAATTTAAATAGGTG14310.10594796777582566No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14280.1057258546358344No Hit
GTCCTGATCTATGGTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTT14200.10513355292919106No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGAATTCCCTGGCCCCACGGGTCGAACCAGTTGGGGC14130.10461528893587815No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14090.10431913808255648No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13830.10239415753596566No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)