FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175022
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44080.37514191223653687No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44050.3748865978679548No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33020.28101601501929324No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32090.2731012695932502No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30880.2628035900604414No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28740.2445911651015896No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27420.23335733288398003No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26220.22314475814069865No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC26100.2221235006663705No Hit
GAGTTCACTCTCACCATCAGCAGCGTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAACAATTTAATAGCTGG25220.21463427918796416No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24780.21088966844876098No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGGTACCAGGGACCCGGCT23940.20374086612846395No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22990.19565591112336622No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21760.18518802201150275No Hit
CTTCTATGGTGCATCCAGCCGGGTCCCTGGTACCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA21340.18161362085135427No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21230.18067746816655347No Hit
GTTTATTACTGTCAACAATTTAATAGCTGGCCTTCTTGGACGTTCGGCCA20600.17531586642633074No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19920.16952874073847127No Hit
GGAGAAATAGTGCTGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG19760.16816706410603374No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19740.16799685452697907No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCGTGCAGTCTGAAGATT19330.16450755815635792No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT18840.16033742346951801No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18440.15693323188842423No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18250.15531624088740467No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17720.1508056870424554No Hit
CCATAGAAGAGGAGTCTGGGAGACTGGCCAGGTTTGTGCTGGTACCAGGC17520.14910359125190847No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17230.14663555235561548No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC17040.14501856135459593No Hit
GTCTGAAGATTTTGCAGTTTATTACTGTCAACAATTTAATAGCTGGCCTT16770.1427207320373576No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC16760.14263562724783024No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16280.1385505973505177No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16050.13659318719138877No Hit
GTAATAAACTGCAAAATCTTCAGACTGCACGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA15940.13565703450658795No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGTTCCTTAGCCTGGTACCAGCACAAACCTGGCCAGTCTCCCAGACT15920.13548682492753328No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15810.13455067224273248No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15800.13446556745320512No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGTTCCTTAGCCTGGTA15330.1304656423454199No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15100.12850823218629098No Hit
CTCCCAGACTCCTCTTCTATGGTGCATCCAGCCGGGTCCCTGGTACCCCA14620.12442320228897841No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGGTACCA14520.12357215439370496No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT14440.12289131607748621No Hit
GCACAAACCTGGCCAGTCTCCCAGACTCCTCTTCTATGGTGCATCCAGCCGGGTCCCTGGTACCCCAGCCAGGTT14400.12255089691937683No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14340.12204026818221277No Hit
GTGTTAGCAGTTCCTTAGCCTGGTACCAGCACAAACCTGGCCAGTCTCCC14280.12152963944504869No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14170.12059348676024789No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14100.11999775323355648No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14070.11974243886497445No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13960.11880628618017365No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13650.11616803770482596No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13620.11591272333624392No Hit
TCCTTAGCCTGGTACCAGCACAAACCTGGCCAGTCTCCCAGACTCCTCTTCTATGGTGCATCCAGCCGGGTCC13550.11531698980955249No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13240.1126787413342048No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13130.111742588649404No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13010.11072133117507588No Hit
CTGGTACCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT12990.11055112159602118No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12960.11029580722743913No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12940.11012559764838445No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC12780.10876392101594692No Hit
AACCTGGCCAGTCTCCCAGACTCCTCTTCTATGGTGCATCCAGCCGGGTC12760.10859371143689224No Hit
GTCAGAGTGTTAGCAGTTCCTTAGCCTGGTACCAGCACAAACCTGGCCAGTCTCCCAGACTCCTCTTCTATGGTG12620.10740224438350941No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12170.10357252885477888No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12160.10348742406525153No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12110.10306190011761482No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12090.10289169053856013No Hit
CTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCT12090.10289169053856013No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT12070.10272148095950545No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11960.10178532827470464No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11830.10067896601084916No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11820.10059386122132181No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11790.10033854685273977No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)