FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175022
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44660.38007799002912285No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42690.3633123464922359No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37820.3218663139924189No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35280.30024969745247326No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32300.2748884701733244No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26780.22791062635422996No Hit
GAGTTCACTCTCACCATCAGCAGCGTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAACAATTTAATAGCTGG26240.22331496771975334No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25500.21701721329472978No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25300.2153151175041829No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC25170.21420875524032743No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24400.20765568644672186No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24150.2055280667085382No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22600.19233682433179974No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGGTACCAGGGACCCGGCT22320.1899538902250341No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCGTGCAGTCTGAAGATT22050.18765606090779577No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21260.1809327825351355No Hit
CTTCTATGGTGCATCCAGCCGGGTCCCTGGTACCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA20820.17718817179593233No Hit
GTTTATTACTGTCAACAATTTAATAGCTGGCCTTCTTGGACGTTCGGCCA20470.17420950416247527No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20310.17284782753003775No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19930.16961384552799863No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19580.16663517789454155No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19080.1623799384181743No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18990.1616139953124282No Hit
GGAGAAATAGTGCTGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG18660.1588055372580258No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT17810.15157163014820146No Hit
GTCTGAAGATTTTGCAGTTTATTACTGTCAACAATTTAATAGCTGGCCTT17180.14621002840797875No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17010.14476324698601387No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC16970.1444228278279045No Hit
GTAATAAACTGCAAAATCTTCAGACTGCACGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA16910.14391219909074043No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16700.14212499851066618No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16230.13812507340288097No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16010.13625276803327938No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGTTCCTTAGCCTGGTACCAGCACAAACCTGGCCAGTCTCCCAGACT15870.13506130097989655No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGTTCCTTAGCCTGGTA15670.13335920518934966No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15480.1317422141883301No Hit
CCATAGAAGAGGAGTCTGGGAGACTGGCCAGGTTTGTGCTGGTACCAGGC15230.12961459445014648No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC15050.12808270823865425No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGGTACCA15040.12799760344912692No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14830.12621040286905266No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT14810.12604019328999796No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14700.12510404060519717No Hit
CTCCCAGACTCCTCTTCTATGGTGCATCCAGCCGGGTCCCTGGTACCCCA14260.121359429865994No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14000.11914670533828303No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13780.11727439996868144No Hit
GTGTTAGCAGTTCCTTAGCCTGGTACCAGCACAAACCTGGCCAGTCTCCC13670.11633824728388065No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13650.11616803770482596No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13510.11497657065144312No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13210.11242342696562277No Hit
GCACAAACCTGGCCAGTCTCCCAGACTCCTCTTCTATGGTGCATCCAGCCGGGTCCCTGGTACCCCAGCCAGGTT13140.11182769343893136No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13010.11072133117507588No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC12900.10978517849027508No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12870.10952986412169305No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12810.10901923538452897No Hit
GTCAGAGTGTTAGCAGTTCCTTAGCCTGGTACCAGCACAAACCTGGCCAGTCTCCCAGACTCCTCTTCTATGGTG12710.10816818748925552No Hit
CTGGTACCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT12550.106806510856818No Hit
AACCTGGCCAGTCTCCCAGACTCCTCTTCTATGGTGCATCCAGCCGGGTC12480.10621077733012657No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12400.10552993901390781No Hit
TCCTTAGCCTGGTACCAGCACAAACCTGGCCAGTCTCCCAGACTCCTCTTCTATGGTGCATCCAGCCGGGTCC12060.10263637616997809No Hit
CTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCT11910.10135980432706793No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11850.10084917558990385No Hit
CTGCTACTCTGGCTCCCAGTTTCAGATAGCACTGGAGAAATAGTGCTGAC11810.10050875643179445No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)