FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences728364
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAGACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTC63140.8668742551801023No Hit
GGTCTACATAGTGCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT48320.663404561455536No Hit
GTCTACATAGTGCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA47910.6577755078504703No Hit
CTATGTAGACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT42210.5795179333410218No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41000.5629053605065599No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36050.49494483527467037No Hit
GTAATGGACACAACTTTTTGAATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTT26600.36520201437742666No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25860.3550422590902351No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24860.34131286005348976No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG24240.33280063265070764No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA23320.3201695855369019No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGACACAACTTTTTGAATTGGTACCTGCAGAAGCCAGGGCAGTCTC22490.30877418433640325No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21340.2929853754441461No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21060.28914114371385735No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT20940.28749361582944793No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20030.2749998627060096No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19210.2637417554958784No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19070.26181963963073407No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT18960.2603094057366921No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18950.26017211174632465No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18620.25564141006419866No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18490.2538565881894218No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18220.25014965044950055No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG16850.2313403737691594No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA16520.22680967208703343No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16270.22337732232784704No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTA16140.2215925004530702No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCC16100.22104332449160036No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16030.22008226655902818No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC15580.21390403699249275No Hit
GCATAGTAATGGACACAACTTTTTGAATTGGTACCTGCAGAAGCCAGGGC15450.21211921511771586No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT14960.20539180958971062No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC14510.19921358002317524No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC14270.19591852425435632No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA14070.19317264444700727No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13930.1912505285818629No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13890.19070135262039312No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13850.19015217665892328No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG13500.1853468869960624No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG13390.18383665310202044No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA13170.18081618531393645No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12570.1725785458918892No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC12180.16722408026755853No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG11800.1620069086335953No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG11620.15953561680698114No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11600.15926102882624624No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11530.15829997089367404No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11420.15678973699963206No Hit
AGTCAGAGCCTCCTGCATAGTAATGGACACAACTTTTTGAATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAA11310.15527950310559005No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11300.1551422091152226No Hit
GTTTATTACTGCATGCAAGGTCTACATAGTGCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA11280.1548676211344877No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11210.1539065632019155No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA11010.15116068339456645No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT10990.15088609541383155No Hit
CTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT10770.14786562762574756No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10660.14635539373170559No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10550.14484515983766358No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT10360.142236574020682No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10260.14086363411700745No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10250.14072634012663998No Hit
GTTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATTCAAAAAGTTGTGTCCATTACTATGCAGGAGGCTCTGA10230.14045175214590508No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10220.1403144581555376No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10170.13962798820370034No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9980.13701940238671872No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9930.13633293243488145No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9860.13537187450230928No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9830.13495999253120694No Hit
CTCCTGCATAGTAATGGACACAACTTTTTGAATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGAT9650.13248870070459276No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA9630.13221411272385786No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9600.1318022307527555No Hit
GCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT9440.1296055269068762No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTACATAGTGCGCTCACTTTCGGCGGAGGGA9390.12891905695503897No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9390.12891905695503897No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9340.1282325870032017No Hit
GTCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC9220.12658505911879225No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9210.12644776512842482No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9150.12562400118622008No Hit
TTATTACTGCATGCAAGGTCTACATAGTGCGCTCACTTTCGGCGGAGGGA9070.12452564926328044No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT9050.12425106128254554No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9020.12383917931144317No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9000.12356459133070828No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8980.12329000334997336No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8900.12219165142703374No Hit
GCCCTGGCTTCTGCAGGTACCAATTCAAAAAGTTGTGTCCATTACTATGC8900.12219165142703374No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG8860.12164247546556392No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8810.12095600551372665No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8740.11999494758115448No Hit
CTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT8730.11985765359078702No Hit
TTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTC8720.11972035960041956No Hit
GATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATTCAAAA8570.11766094974490778No Hit
GGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTA8430.11573883387976341No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8370.1149150699375587No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8330.11436589397608887No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT8320.11422859998572142No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8200.11258107210131198No Hit
GGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGG8180.11230648412057709No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8150.11189460214947472No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC8120.11148272017837235No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC7950.10914872234212565No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7830.10750119445771619No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7800.10708931248661384No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT7760.10654013652514403No Hit
CCACAACTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGA7750.10640284253477658No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7650.10502990263110204No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTACATAG7640.10489260864073459No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG7600.10434343267926476No Hit
CCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG7470.10255861080448786No Hit
GGCTTCTGCAGGTACCAATTCAAAAAGTTGTGTCCATTACTATGCAGGAG7390.10146025888154823No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTC150.002584106367.404556
AATCGAG200.007008143752.3912647
GTAGTTT400.00741602951.6417670
ATCGCGT200.007620605651.2928637
AATATCG200.00767531551.20013436
GTATTGA200.00805556450.5777131
GTGGTAT1200.050.5777131
CGTATGC3950.049.8134241
GTATGCC4050.048.69645342
CCGTCTT3950.048.63323247
ATGCCGT3950.048.3545844
ATCTCGT3400.048.27562737
TCGTATG3650.048.11139740
CTTATAC4450.047.7362671
AATCTCG3450.047.4899836
ACGAGAC4650.047.15278222
TAATCTC3550.047.02872535
CCCACGA4600.046.9126619
ATCTCCG4650.046.38592510
TATACAC4650.046.3859253
Produced by FastQC (version 0.11.5)