FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1592238
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATAGTTATTCTCCCTCGTACACTTTTGGCCAGGGGACCAAGCTGG49880.3132697498740766No Hit
CTATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC46130.2897179944204321No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45140.28350033098066996No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGTTCTTGATCTATAAGGCGT44980.2824954560813145No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44660.2804857062826035No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34950.21950236082796665No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32930.2068158152236035No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA30760.19318719940109455No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30080.1889164810788337No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28010.17591591206842194No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC27880.1750994512126956No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27660.17371774822608177No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26380.1656787490312378No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26220.1646738741318823No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25300.1588958434605882No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25250.1585818200545396No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24830.1559440234437314No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG24510.1539342736450204No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24420.15336903151413295No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA24400.1532434221517135No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAAAAA23910.15016599277243728No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT23340.1465861259434833No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA22300.1400544390976726No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA22190.13936358760436568No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG21520.13515567396331454No Hit
GGCCAAAAGTGTACGAGGGAGAATAACTATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGG21260.13352275225186186No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAAATGTGACAT20140.12648862795637336No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20050.1259233858254859No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19790.1242904641140332No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19490.12240632367774165No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19390.12177827686564446No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19110.12001974579177234No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCT19110.12001974579177234No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18920.11882645684878769No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18790.11800999599306133No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18670.11725633981854472No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18590.11675390236886697No Hit
CACTATAATAGTTATTCTCCCTCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCA18090.11361366830838103No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17600.11053623892910482No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17490.10984538743579791No Hit
CCTTATAGATCAAGAACTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCCAACCAGCTACTAATACTCTGA17450.10959416871095903No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17220.10814966104313552No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC17160.1077728329558772No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16870.10595149720079536No Hit
ATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC16810.10557466911353705No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16810.10557466911353705No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAAGAACTTAGGGGCTTTCCCTGGTTTTTG16680.10475820825781071No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC16510.10369052867724549No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16020.10061309929796927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG6400.036.60439340
ATATAAG400.002004766836.36773762
CGTATGC6700.035.0338141
TATACAC7450.034.4716073
ACGAGAC6900.034.303622
ATGCCGT7050.033.9825744
ATCTCGT5650.033.94525537
CACGAGA7100.033.81248521
CTTATAC7600.032.919581
TGCCGTC7100.032.82556545
GTATGCC7350.032.46666742
CGAGACT7200.032.4107623
TTATACA7550.032.2247662
TAGGCAT7300.032.05644629
ACTAGGC7300.032.01809727
GTATCAA32150.031.6534961
GAGACTA7400.031.54371624
CCGTCTT7400.031.12837647
GCCGTCT7300.031.02235246
AGACTAG7550.030.9257725
Produced by FastQC (version 0.11.5)