FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1790983
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA82740.46198093449239885No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53400.2981602840451305No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48790.2724202295610846No Hit
GAGTTACAGTACCCCTTGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT45780.25561381654655574No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA36910.20608794164992073No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCGTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT35460.19799182906817095No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35280.19698679440285027No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG34320.19162660952114005No Hit
GTGTATTACTGTGCGAGGCACCTCAGAATGACTACGGTGACCCCCTTTGA33940.18950486967212976No Hit
ACAGTACCCCTTGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA33840.18894651708028495No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32660.18235795649651615No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA32200.17978953457403002No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32090.17917534672300073No Hit
GTATTACTGTGCGAGGCACCTCAGAATGACTACGGTGACCCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCAC30260.16895749429224063No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28540.15935382971250983No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28380.15846046556555812No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC27620.15421698586753754No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27550.15382613905324619No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT27170.1517043992042359No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTGACTACTACATGAGCTGGATCCGCCAGGCTCCAGGGAAGGGG27150.15159272868586693No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGTAGTTATTTAAATTGGTATCAGCACAAA26530.1481309426164291No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26160.14606503802660326No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25830.14422247447351538No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCGTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC25740.14371995714085506No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25730.14366412188167058No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT25710.1435524513633016No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25700.14349661610411713No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25640.14316160454901022No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTACTCTGG25290.14120737047755338No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC24690.13785725492648448No Hit
AACCAGGGAGAGCCCCTAACCTCCTGATCTACGGTGCATCCAGTTTGCAA24640.1375780786305621No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24250.13540050352236732No Hit
GTGTATTACTGTGCGAGGCACCTCAGAATGACTACGGTGACCCCCTTTGACTACTGGGGCCAGGGAACCCTGGT22830.12747189671817097No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22530.12579683894263652No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT22500.1256293331650831No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG22470.12546182738752965No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG22240.12417761642628657No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCT22040.12306091124259695No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC21510.12010164250581942No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGCAAGGGGTA21060.11758905584251776No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20950.11697486799148848No Hit
GATCTACGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAACTTCAGTGGCAGTGGATCTGGGACAGATTTCA20850.11641651539964366No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT20660.11535564547513852No Hit
CTACTACATGAGCTGGATCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGG20210.11284305881183686No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20150.11250804725672996No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGACGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACC20150.11250804725672996No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCTTGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA19690.10993962533424383No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19650.10971628429750589No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG19030.10625449822806805No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18860.10530529882193188No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18640.10407692311987327No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18570.1036860763055819No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG18500.10329522949129055No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18480.10318355897292159No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18450.10301605319536812No Hit
GTAGTAAGTTGCAAAATCGTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA18160.10139683067901817No Hit
GCTCCTGGGACTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC18080.10095014860554233No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18030.10067097230961992No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)