FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1790983
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA73930.4127900711508708No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52060.29067835931441005No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47130.2631515765364607No Hit
GAGTTACAGTACCCCTTGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT43280.2416550017504354No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37780.21094560919897062No Hit
GTGTATTACTGTGCGAGGCACCTCAGAATGACTACGGTGACCCCCTTTGA35380.19754514699469508No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35310.1971543001804037No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34800.19430670196199518No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCGTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT34590.19313416151912108No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG32930.18386550849449718No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA32780.18302797960672992No Hit
GTATTACTGTGCGAGGCACCTCAGAATGACTACGGTGACCCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCAC30350.16946001162490096No Hit
ACAGTACCCCTTGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA30250.16890165903305615No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTGACTACTACATGAGCTGGATCCGCCAGGCTCCAGGGAAGGGG28650.15996801756353912No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28410.15862797134311157No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26640.14874513046745838No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26110.14578586173068087No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT25600.14293826351227232No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25320.14137487625510683No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGTAGTTATTTAAATTGGTATCAGCACAAA25160.14048151210815513No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25130.14031400633060168No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24480.13668471448361041No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24140.13478631567133803No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCGTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC23670.1321620584896674No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT23410.13071034175087087No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23400.13065450649168642No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC23360.13043116545494848No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23350.130375330195764No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTACTCTGG22830.12747189671817097No Hit
GTGTATTACTGTGCGAGGCACCTCAGAATGACTACGGTGACCCCCTTTGACTACTGGGGCCAGGGAACCCTGGT22790.12724855568143303No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC22540.125852674201821No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC22250.12423345168547106No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG22240.12417761642628657No Hit
AACCAGGGAGAGCCCCTAACCTCCTGATCTACGGTGCATCCAGTTTGCAA22000.12283757020585902No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCT21770.12155335924461594No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21560.12038081880174183No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21010.11730987954659536No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20600.11502063392003162No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20330.11351308192205063No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20120.11234054147917652No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCTTGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA19680.10988379007505933No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19610.10949294326076796No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19500.10887875540973868No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGCAAGGGGTA19420.10843207333626281No Hit
GATCTACGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAACTTCAGTGGCAGTGGATCTGGGACAGATTTCA19340.10798539126278697No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGACGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACC19220.1073153681525732No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC19060.10642200400562149No Hit
CTACTACATGAGCTGGATCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGG19040.10631033348725252No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19000.10608699245051462No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18590.10379774682395086No Hit
GCTCCTGGGACTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC18490.10323939423210605No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGACTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT18330.10234603008515435No Hit
GTCTGCAACCTGACGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCCCTTGCACTTTTGGCCAGGGGA18160.10139683067901817No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTTCCATTCGGTGAT18080.10095014860554233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG25350.044.9480470
TACGTAT804.6313155E-543.68990770
GTATCAA40250.037.5488131
CGTGCGT800.002435480534.95192770
TGTTAGA14450.034.83098270
ATCAACG43550.034.543983
TCAACGC44050.034.151884
GTGGTAT4100.034.1160161
TATCAAC44850.033.697652
CAACGCA44650.033.6146745
AACGCAG45100.033.279276
ATACGTA1800.032.21258511
CGCAGAG48150.031.242088
AGAGTAC48650.030.84742711
GCAGAGT50400.029.8473459
GAGTTTA5900.028.9118791
GACAGGT4750.028.7292751
TACGTAC1900.028.72125212
GTGCTAG2450.027.849811
GTACGTA955.8984646E-527.77929369
Produced by FastQC (version 0.11.5)