FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences983710
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT39970.40631893545862097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG9050.047.33518270
CTAACGC1000.046.86993
TCGTATG8650.042.7535140
CTCGTAT7750.041.34256739
CGTATGC9350.040.79438841
GCCGTCT9100.038.29004346
ATCTCGT8200.038.018737
TAACGCC1150.037.845264
TCTCGTA8150.037.52248438
CCGTCTT9300.037.19348547
CTTATAC11350.037.1825681
TATGCCG10050.037.17822343
TGCCGTC9650.037.08693345
CGAGACT11050.037.0078323
TCCCGCA4650.036.8501970
AATCTCG8500.036.59691636
CGTCTTC8750.036.4194648
TATACAC11900.036.291823
ACGAGAC11450.036.2838522
GCTAATC9100.035.86116433
Produced by FastQC (version 0.11.5)